Abstract
H.A. Zaviryukha, T.B. Vasylieva, U.M. Yanenko*, V.A. Synytsyn, N.I. Kosyanchuk, N. H. Sorokina, O.L. Kravtsova, L.M. Muzykina and O.O. Marchuk
An important test is the study of sensitivity to antibiotics in certain strains, which is created for the anti-anthrax drug as well as the direction of antibiotic therapy in the treatment of the disease. According to the order N 23 from 04.04.02, on the territory of Ukraine it is forbidden to use drugs which contain the following active substances: nitrofurans, including furazolidone, ronidazole, dapsone, chloramphenicol (chloramphenicol), dimetridazole, colchicine, chlorpromazine, chloroform, metronidazole, due to their harmful effects on the body, in particular mutagenic and teratogenic effects. That is why the introduction of antibiotic therapy to avoid the development of resistance to the pathogen B. аnthracis is relevant. The research was conducted on the basis of the State Center for Innovative Biotechnology: B. аnthracoides 67, B. subtilis 17, B. cereus 8035, B. аnthracis Tsenkovsky II IBM 92 Z, B. аnthracis K–79 Z, B. аnthracis Stern 34F2, B. аnthracis 55, B. аnthracis SB, B. аnthracis Tsenkovsky I. We determined the sensitivity to the antibiotic by the size of the diameter of the zones of growth retardation of the strain on nutrient agar. We considered the growth retardation of the strain up to 15 mm as a sign of low sensitivity to the antibiotic, a sign of regulatory sensitivity was considered a zone of 15–24 mm, the zone of more than 24 mm – a sign of high sensitivity of the strain to the antibiotic. In the absence of zones of growth retardation of the microorganism, the strain was insensitive to the antibiotic. According to the results of research it is established that benzylpenicillin does not affect the growth of strains B. аnthracoides 67, B. subtilis 17, B. cereus 8035, B. аnthracis Tsenkovsky II IBM 92 Z, B. аnthracis 55, B. аnthracis SB, B. аnthracis Tsenkovsky I. Benzylpenicillin has a weak effect on strains of B. аnthracis K–79 Z and B. аnthracis Stern 34F2. Vaccine strains of B. аnthracis K–79 Z, B. аnthracis Stern 34F2, B. аnthracis 55, B. аnthracis SB showed high sensitivity to amoxiclav, tetracycline, norfloxacin. The vaccine strain of B. аnthracis Stern 34F2 showed high sensitivity to furazalidone. We plan to continue the study of antibiotic susceptibility of B. аnthracis strains in the future, in order to prevent antibiotic resistance in the treatment and prevention of the disease.