Accumulation and identification of secondary metabolites from the fungus Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al.


Ye.V. Syvoded, O.V. Kolesnichenko, S.M. Hrysiuk, R.V. Ivannikov

The study presents variability in the qualitative composition and quantitative indicators of secondary mycelium metabolites of the fungus Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al., the most harmful plant pest of Helianthus annuus L. Toxicity of secondary metabolites of D. helianthi was analyzed by determining the average length of seedling shoots of Triticum aestivum L. test object grown on fungus filtrates of different cultivation dates. The maximum toxic effect of D. helianthi was recorded during the germination of Triticum aestivum L. seedlings on a 17-day filtrate of pure mycelium culture of the fungus. The mean length of wheat seedlings in this variant of the experiment decreased by 3.5 times compared with the control and 3.3 times compared with the filtrate of the culture medium of 7 days of cultivation. High-performance liquid chromatography (HPLC) identified secondary metabolites of D. helianthi as fomosin, fomopsolides, cytosporones, and xanthones. There was a redistribution of secondary metabolites due to increase in number of cytosporones, decrease in content of fomopsolides, and termination of xanthone synthesis with increasing time of cultivation of fomopsis mycelium, under conditions of relatively stable indicators of fomosin content.

Keywords: Diaporthe (Phomopsis) helianthi ??.; Secondary metabolites; Mycotoxins


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