Antibiotic susceptibilty of bacteria from the Bacillus subtilis group


A.N. Irkitova, A.V. Grebenshchikova, D.E Dudnik

The ability of bacteria to quickly acquire antibiotic resistance and transfer it among different groups are created a global problem, since antibiotics are used not only in medicine, but also in agriculture. That is why to study of the antibiotic sensitivity of not only pathogenic bacteria, but also of beneficial microbes like Bacillus spp. is extremely important. Our results demonstrated that all the studied strains of the B. subtilis group were highly sensitive to cephalexin and enrofloxacin, while resistant to oxacillin. The B. subtilis B-5449 strain was the least resistant to the studied antibiotics, and the B. subtilis B-2896 strain was the most resistant.
Key words: Bacillus subtilis; Bacillus pumilus; antibiotic sensitivity; antibiotics.
Alonso, A., Sanchez, P., Martinez, J.L. (2001). Environmental selection of antibiotic resistance genes. Environmental microbiology, 3, 1-9. doi:10.1046/j.1462-2920.2001.00161.x
Andrews, J.M., Wise, R. (2002). Susceptibility testing of Bacillus species. Journal of Antimicrobial Chemotherapy, 49(6), 1040-1042. doi: 10.1093/jac/dkf063
Balouiri, M., Sadiki, M., Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. doi: 10.1016/j.jpha.2015.11.005
Bush, K. (2009) The Importance of β-Lactamases to the Development of New β-Lactams. In: Mayers DL (ed) Antimicrobial drug resistance. Humana Press, New York, pp 135-144. doi 10.1007/978-1-59745-180-2_12.
Bush, K., Bradford, P.A. (2016). β-Lactams and β-Lactamase Inhibitors: An Overview. Cold Springs Harbor Perspectives in Medicine, 6(8), a025247. doi: 10.1101/cshperspect.a025247
Guidelines (MUK) 4.2.1890-04 (2004). Determination of the sensitivity of microorganisms to antibacterial drugs. Guidelines. Moscow: Federal Center for State Sanitary and Epidemiological Supervision of the Ministry of Health of Russia.
Irkitova, A.N., Grebenshchikova, A.V. (2018). Antimicrobial action of a bacterial consortium containing strains of the genus Bacillus. Ukrainian Journal of Ecology, 8(4), 444-449.
Ismail, F., Adeloju, S.B. (2010). Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin. Sensors, 10(4), 2851-2868. doi: 10.3390/s100402851 Li, X.Z., Nikaido, H. (2009). Efflux-mediated drug resistance in bacteria: an update. Drugs, 69(12), 1555-1623. doi:10.2165/11317030-000000000-00000 Martinez, J.L., Baquero, F. (2000). Mutation Frequencies and Antibiotic Resistance. Antimicrobial Agents and Chemotherapy, 44 (7), 1771-1777; doi: 10.1128/AAC.44.7.1771-1777.
Martinez, J.L., Sanchez, M.B., Martinez-Solano, L.E., Hernandez, A.G., Garmendia, L., Fajardo, A.T., Alvarez-Ortega, C. (2009). Functional role of bacterial multidrug efflux pumps in microbial natural ecosystems. FEMS microbiology reviews, 33, 430-449. doi:10.1111/j.1574-6976.2008.00157.x Nikaido, H. (2009). Multidrug resistance in bacteria. Annual review of biochemistry, 78, 119-146. doi:10.1146/annurev.biochem.78.082907.145923
Owusu-Kwarteng, J., Wuni, A., Akabanda, F., Tano-Debrah, K., Jespersen, L. (2017). Prevalence, virulence factor genes and antibiotic resistance of Bacillus cereus sensu lato isolated from dairy farms and traditional dairy products. BMC Microbiology, 14, 65. doi: 10.1186/s12866-017-0975-9
Selizarova, N.O. (2003). Antibiotics that disrupt the synthesis of macromolecules. Clinical Pharmacology and Drug Therapy Reviews, 1(1), 71-79.
Semenov, V.M., Zhyltsov, I.V., Dmitrachenko, T.I., Skvortsova, V.V., Zenkova, S.K., Vasilyeva, M.A. (2013). Beta-lactamase activity is the main cause of bacterial resistance to beta-lactam antibiotics. Clinical Infectology and Parasitology, 3(06), 52-65.
Sidorenko, S.V., Tishkov, V.I. (2004). Molecular basis of antibiotic resistance. Advances in Biological Chemistry, 44, 263-306.
Trouchon, T., Lefebvre, S. (2016). A Review of Enrofloxacin for Veterinary Use. Open Journal of Veterinary Medicine, 6, 40-58. doi: 10.4236/ojvm.2016.62006
Vinogradova, K.A., Bulgakova, V.G., Polin, A.N., Kozhevin, P.A. (2013). Microbial Antibiotic Resistance: Resistome, Its Volume, Diversity and Development. Antibiotics and Chemotherapy, 5-6, 38-48.
Zakharochkina, E.R. (2013). Vital and essential drugs: antibiotics. Remedium. Magazine on the Russian market of medicines and medical equipment, 6, 34-43.
Zheldakova, R.A. (2004). The mechanisms of biosynthesis of antibiotics and their effect on microorganism cells. Minsk: BSU, 111.

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