Assessment Ukrainian breeds ducks genetic diversity by microsatellite loci

Abstract

A.M. Chepiha, S.O. Kostenko, M.S. Doroshenko, O.M. Konoval, L. Lu, O.V. Sydorenko*, N.P. Svyrydenko, P.P. Dzhus and M.V. Drahulian

Genetic pools of local duck breeds play an essential role as a source of gene polymorphism alleles, which are associated with valuable economic characteristics. Local breeds are represented primarily by Ukrainian White, Ukrainian Clay, Ukrainian Grey, and Ukrainian Black White-Breasted. Two breeds Ukrainian Clay and Ukrainian Black White-Breasted, were studied using a panel of 21 pairs of primers by standard method for DNA isolation, electrophoresis, and statistical analysis. The value of Na in the population of the Ukrainian Clay breed ranged from 2 (CAUD011) to 10 (CAUD050). The highest genetic diversity in the duck population of the Ukrainian Clay breeds was observed by locus CAUD050. According to the results of microsatellite analysis, the number of alleles in the duck population of the Ukrainian Black White-Breasted breed was highest by locus CAUD069. On average, the number of alleles per locus for the Ukrainian Clay breed ducks was 4.429 ± 0.563, while it was slightly higher for the Black White-Breasted breed, i.e., 4.810 ± 0.563. The data obtained can be used to develop biodiversity preservation programs and breeding programs to improve the existing and breed new breeds or crosses of ducks.

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