Biochemical and molecular-genetic markers of adaptability and quality of genotypes in cultural and wild cereal plants
V. V. Moskalets, A. H. Vovkohon, M. M. Kliuchevych, T. Z. Moskalets, A. O. Sliusarenko, V. V. Liubych, A. T. Martyniuk, O. S. Pushka, I. M. Pushka, V. I. Nevlad
We proved that plants A. cylindrica, T. spelta, T. turgidum, T. sphaerococcum, T. vavilovii, T. persicum, T. araraticum, T. aestivum var. barbarossa, and T. aestivum var. ferrugineum, owning high drought resistance, have a stable high productive potential and quality indicators of grain (protein, gluten, dietary fiber content), that allows use them in the production of products functional purpose (bakery, confectionery, sour-dairy products, including yoghurts, and dessert cheese). Implementation mechanisms are disclosed adaptive potential plants of cultural and wild cereals by biochemical (protein content, gluten, starch in seeds, the presence of gliadin proteins (Glі-1В1, Glі-6D2, Glі-6D3, Glі-6В2), molecular-genetic markers (allele genes of drought resistance – Dreb А1, Dreb В1, Dreb D1; glutenins Glu-D1) and morphological-ontogenetic criteria. For the first time for successful address introduction cultural and wild cereal plants suggested by the criteria of mechanisms drought resistance to rank plants by potential ecological valence regarding the unfavorable hydrothermal regime. Differentiation of genotypes based on elements of plant strategies: features of their ontogeny, morphological characteristics, physiological and biochemical parameters, biochemical and molecular-genetic markers. In this connection, genotypes of cultural and wild cereal plants in conditions in sіtu by sensitivity on the effect of hydrothermal stress are divided into xerophytic, mesophytic and intermediate – xerophytic-mesophytic types of development. That's it ranking underlying on the principles of autecological approach, manifestation of a multi-level system of answers plant organisms at different levels of integration, as adaptive reactions. In particular, mechanisms such as: functional stability (stipulated structural and functional features of plants), morphological tolerance (the ability of plants to resist damage without reducing performance), ontogenetic evasion (stability due to features ontogenetic development), ecological plasticity of the organism and plant populations as a whole. The obtained results of the biochemical and molecular genetic markers of adaptability and quality indicators of genotypes cultural (wheat, triticale, rye) and wild cereals (A. cylindrica, T. spelta, T. turgidum and others) are important in breeding and successive targeted use in the food industry are given.
Keywords: Wheat species; Rye; Amphidiploids; Adaptive properties; Alleles of genes Dreb 1; Glu-D1; Gliadin-proteins Glі; Grain quality indicators
Avdeev, V. (2015). Modern methods of biometrics in the study of plants. Orenburg: Publishing Center ОSАU, 130 (in Russian).
Bita, C., Gerats, T. (2013). Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant crops. Frontiers in Plant Science, 4, 273. doi: 10.3389/fpls.2013.00273.
FAO World Food and Agriculture Statistical Yearbook. (2019). Available from: http://www.fao.org/3/i3107e/i3107e.pdf. Accessed on 02.10.2019.
Ghazi, A.I., Zanouny, A.I., Moustafa, K.A., AI-Doss, A.A. (2012). Molecular screening of high molecular weight glutenin genes in spring bread wheat genotypes in Saudi Arabia. Journal of Food, Agriculture & Environment, 10, 157-161.
Hussain, H.A., Hussain, S., Khaliq, A., Ashraf, U., Anjum, S.A., Men, S., Wang, L. (2018). Chilling and Drought Stresses in Crop Plants: Implications, Cross Talk, and Potential Management Opportunities. Frontiers in Plant Science, 9. doi: 10.3389/fpls.2018.00393
Intergovernmental Panel on Climate Change, 2014. (2014). Climate Change 2014: Impacts, Adaptation, and Vulnerability (eds Field C. B. et al.) [serial online]. Cambridge University Press. doi:10.1017/CBO9781107415324. Accessed on 20.11.2019.
Liubych, V., Novikov, V., Polianetska, I., Usyk, S., Petrenko, V., Khomenko, S., Zorunko, V., Balabak, O., Moskalets, V., Moskalets, T. (2019). Improvement of the process of hydrothermal treatment and peeling of spelt wheat grain during cereal production. Eastern-European Journal of Enterprise Technologies, 3(11), 40-51. DOI: 10.15587/1729-4061.2019.170297
Liu, S. X., Chao, S. M., Anderson, J. A. (2008). New DNA markers for high molecular weight glutenin subunits in wheat. Theoretical and Applied Genetics, 118, 177-183. doi: 10.1007/s00122-008-0886-0
Liu, Meng, Wang, Zeng, Xiao, Hong-mei. (2018). Characterization of TaDREB1 in wheat genotypes with different seed germination under osmotic stress. Hereditas, 155, 26. doi: 10.1186/s41065-018-0064-6
Moskalets, T.Z., Rybalchenko, V.K. (2016). Conceptual model of management the vital state plant eсomorрhs by the criteria of mechanisms adaptability. Visnyk of Dnipropetrovsk University. Biology, ecology. 24(1), 211-221 (in Ukrainian). doi:10.15421/011626
Moskalets, T.Z., Vasylkivskyi, S.P., Morgun, B.V., Moskalets, V.І., Moskalets, V.V., Rybalchenko, V.K. (2016). New genotypes and technological indicators of winter triticale. Biotechnologia Acta, 9(1), 79-86 (in Ukrainian). doi: 10.15407/biotech9.01.079
Morgounov, A.I., Gummadov, N., Belen, S., Kaya, Y., Keser, M., Mursalova, J. (2014). Association of digital photo parameters and NDVI with winter wheat grain yield in variable environments Turk. Journal of Agriculture and Food Research, 38, 624-632. DOI: 10.3906/tar-1312-90
Mykhailyk, S. Yu., Antonyuk, M. Z., Ternovska, Т. К. (2014). Possible molecular mechanisms of variability in gliadin genes in the wheat introgressive lines. Journal Factors in Experimental Evolution of Organisms, 14, 62-66. (In Ukrainian).
Noman, A., Ali Q., Naseem, J., Javed, M.T., Kanwal, H., Islam, W., Aqeel, M., Khalid, N., Zafar, S., Tayyeb, M., et al. (2018). Sugar beet extract acts as a natural bio-stimulant for physio-biochemical attributes in water stressed wheat (Triticum aestivum L.). Acta Physiologiae Plantarum, 40, 110. doi: 10.1007/s11738-018-2681-0
Оstеrmаn, L. А. (2002). Methods of study of proteins and nucleic acids. Мoskva: МTsNМО, 248 (in Russian).
Parent, B., Vile, D., Violle, C., Tardieu, F. (2016). Towards parsimonious ecophysiological models that bridge ecology and agronomy. New Phytologist, 25. doi: 10.1111/nph.13811
Payne, P. I., Lawrence, G. J. (1983). Catalologue of alleles for the complex gene loci Glu-A1, Glu-B1, Glu-D1 wich code for high-molecular-weight subunits of glutenin in hexaploid weat, 11, 29-35.
Pоpieriеlia, Fh. А. (1989). Gliadin polymorphism and its relationship with grain quality, productivity and adaptive properties of soft wheat varieties. Мoskva: “Agropromizdat”, 138-150 (in Russian).
Qaseem, M.F., Qureshi, R., Muqaddasi, Q.H., Shaheen, H., Kousar, R., Röder, M.S. (2018). Genome-wide association mapping in bread wheat subjected to independent and combined high temperature and drought stress. PLoS One, 13. doi: 10.1371/journal.pone.0199121
Rogozhin, V.V., Rogozhina, T.V. (2913). Workshop on the physiology and biochemistry of plants. Petersburg: GIORD, 352 (in Russian).
Sallam, А., Alqudah, A. M., Dawood М. F. A., Baenziger P. S., Börner А. (2019). Drought stress tolerance in wheat and barley: advances in physiology, breeding and genetics research. International Journal of Molecular Sciences, 20(13), 31-37. doi: 10.3390/ijms20133137
Sharma, P., Sareen, S., Saini, M. (2017). Assessing genetic variation for heat stress tolerance in Indian bread wheat genotypes using morpho-physiological traits and molecular markers. Plant Genetic Resources, 15, 539-547. doi: 10.1017/S1479262116000241
Sukumaran, S., Reynolds, M. P., Sansaloni, C. (2018). Genome-wide association analyses identify qtl hotspots for yield and component traits in Durum wheat grown under yield potential, drought, and heat stress environments. Frontiers in Plant Science, 9, 81. doi: 10.3389/fpls.2018.00081
Thabet, S.G., Moursi, Y.S., Karam, M.A., Graner, A., Alqudah, A.M. (2018). Genetic basis of drought tolerance during seed germination in barley. PLoS ONE, 13:e0206682. doi: 10.1371/journal.pone.0206682
Tardieu, F., Parent, B., Caldeira, C., Welcker, C. (2014). Genetic and physiological controls of growth under water deficit. Journal of Plant Physiology, 164 (4), 1628-1635. doi.org/10.1104/pp.113.233353
Tishchyenkо, V. N., Chyеkаlin, N. M., Pаnchyеnkо, I. А., Didyеnkо, S. Yu. (2006). Polymorphism glutenins in accessions of Poltava winter wheat breeding. Visnik Poltava State Agrarian Academy, 3, 6 (in Ukrainian).
Tishchenko, V.N., Panchenko, P.M., Chernysheva, O.P. (2013). Identification of varieties and breeding lines of winter wheat by the balance of quantitative traits using cluster analysis. Visnik Poltava State Agrarian Academy, 3, 28-31 (in Russian).
Wei, B., Jing, R., Wang, Ch. (2009). Dreb1 genes in wheat (Triticum aestivum L.): development of functional markers and gene mapping based on SNPs. Molecular Breeding, 23, 13-22. doi: 10.1007/s11032-008-9209-z