Changes in the biochemical composition of the seed material of sunflower hybrids during long-term storage
O.A. Yeremenko, L.A. Pokoptseva, L.V. Todorova, A.V. Shepel
The crop saving is no less important task for farmers than growing it. Crop losses reach 15 percent when sunflower seeds are stored incorrectly. We have conducted an experiment to determine changes in the biochemical composition of sunflower seeds of Lohos and Persei hybrids during long-term storage. We have used laboratory analytical and mathematical statistical methods of research, according to generally accepted methods. The analyzed research on this problem by both national and foreign scientists indicates the relevance of this issue. Our experiments have not revealed a significant difference in the biochemical composition of different hybrids seeds during storage. Throughout the first 6 months of storage in the sunflower seeds post-harvest maturation occurs. It is set that due to antioxidant components in the seed the peroxide processes are inhibited at the end of the first year of storage. At the end of the second year of storage the content of free fatty acids in the sunflower seed of the investigated hybrids increased and was higher than the initial values. According to the results of laboratory researches it has been proven, that the seed material of sunflower does not have substantial changes in biochemical composition during two years under standard storage conditions.
Keywords: Enzymes; Fatty acids; Sunflower seeds; Vitamins; Storage
Almeida F.A.C. et al. (2010). Estudo de tecnica sparaarm azenamento de cinco oleaginosas em condições ambientais e criogênicas, Revista Brasileira de Produtos Agroindustriais, 12, 189-202. (in Portuguese).
Antonova B.I. et al. (1991). Laboratory research in veterinary medicine: biochemical and mycological: Handbook, Agropromizdat, 23-42. (in Russian).
Baranenko V.V. (2006). Superoxide dismutase in the plant cells. Cytology, 48(6), 465-474. (in Russian).
Baydalinova L.S., Krivich V.S., Bakholdina L.P. (1977). Methodical recommendations and guidelines for gas chromatography of fatty acids, Kaliningrad, 279 p. (in Russian).
Belіngherі C., Gіussanі B., Rodrіguez-Estrada M., Ferrillo A.,Vittadini E. (2015). Oxіdatіve stabіlіty of hіgh-oleіc sunflower oіl іn aporous starch carrіer, Food Chemіstry, 166, 346–351. Retrieved from 10.1016/j.foodchem.2014.06.029. (in English).
Clarence R. (1961). Quick. How long can a seed remain alive? Yearbook of Agriculture, 95-99. (in English).
Desheva G., Petrova S., Deshev M. (2017). Germinability of soybean seeds stored more than 30 years in the Bulgarian national seed genebank, 69, 29-46. (in English).
Dospekhov B.A. (1985). Methods of field experience (with the fundamentals of statistical processing of study results). 5th ed. revised and enlarged. Moscow: Agropromizdat (in Russian).
Ermakova A.N. et al. (1987). Methods of biochemical research of plants, Agropromizdat, 430 p. (in Russian).
Fridowich J. (1995). Superoxide radical and superoxide dismutases, Ann. Rev. Biochem, 64, 97 – 102. (in English).
Gavrilyuk M.M. et al. (2002). Seed production and seed crop oilseeds, Agricultural science, 221 p. (in English).
Jones Q., Earle R. (1966). Chemical analyses of seeds. II: Oil and protein content of 759 species. 20, 127-155. (in English).
Kalenska S.M., et al. (2011). Seed production and methods for determining the guality of seeds of agricultural crops, Tutorial. FOP Danilyuk, 320 p. (in English).
Kalenska S.M., Novytska N.V., Stepanenko Ju.P., Stoliarchuk T.A., Taran V.G., Ryzhenko A.S., Yeremenko O.A. (2017). Longevity of seeds of oily crops, Bulletin of Agricultural Science, 12, 63-70. (in English).
Kolupaev Yu.E., Karpets Yu.V., Obozniy A.I. (2011). Antioxidant plant system: participation in cell signaling and adaptation to the action of stressors. Series Biology, 1, 6-34. (in Russian).
Kozarenko T.D. (1975). Ion exchange chromatography of amino acids, Siberian Branch of the Science, 186 p. (in Russian).
Krasilnikova L.A., Avksentieva O.A., Zhmurko V.V. (2005). Plant biochemistry, Phoenix, 224 p. (in English).
Krestikov I.S. (1990). Aspects of free-radical regulation of proteolytic enzyme activity, VASKHNIL reports, 12, 5 - 8. (in English).
Kropotkin A.V., Prokdi R.G. (2010). Novice. Excel 2010: work with spread sheets and calculations, Science and Technology, 192 p. (in English).
Kryshchenko V.P. (1983). Methods for assessing the guality of plant products, Moscow, Textbook, 192 p. (in Russian).
Кuznetsov V.V., Dmitrieva G.A. (2005). Plant physiology, Graduate School, 73 p. (in Russian).
Marcos Filho J. (2005). Fisiologia de sementes de plantas cultivadas,
Мusienko М.М., Parshikova Т.V., Slavnyy P.S. (2001). Spectrophotometric methods in the practice of physiology, biochemistry and plant ecology, Phytosociocenter, 200 p. (in Russian).
Oil seeds. Acceptance rules and sampling methods: DSTU (GOST) 10852-86. Interstate standard. [Valid from 1987-07-01]. (in Russian).
Oil seeds. Method for determination of moisture content: DSTU (GOST) 10856-96. Interstate standard [Valid from 1997-07-01]. (in Russian).
Oil seeds. Methods of determination of oil content: DSTU (GOST) 10857-2018. Interstate standard [Valid from 2018-09-12]. (in Russian).
Pat. 2144674, Russian Federation, IPC7 G 01 N33/52, G 01 N33/68. A method for determining the antioxidant activity of superoxide dismutase and chemical compounds, №99103192/14; publ. 20.01.2000, 2(2). (in Russian).
Rozhkov A. O., Puzik V. K., Kalensjka S. M., Puzik L. M., Popov S. I., Muzafarov N. M., Bukhalo V. Ja., & Kryshtop Je. A. (2016). Doslidna sprava v aghronomiji [Pilot case in agronomy]. 1, Kharkiv: Majdan, 316. (in Ukrainian).
Sakhno L.O., Ostapchuk A.M., Klochko V.V., Kuchuk M.V. (2012). Fatty acid composition of rapeseed with cytochrome Р450SCC transgene СYP11A1, Biotechnologia Acta, 5(5), 27 - 33. (in English).
Sharma P., Jha A.B., Dubey R.S., Pessarakli M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions [Electronic resource]. Article ID 217037, 26 p. Retrieved from http://dx.doi.org/10.1155/2012/217037. (in English).
Shpaar D., Drager D., Kalenskaya S. (2006). Renewable plant resources, 1, 415 p. (in English).
Takahama U. (2004). Oxidation of vacuolar and apoplastic phenolic substrates by peroxidase: Physiological significance of the oxidation reactions, Phytochem. Rev, 3(1-2), 207-219. (in English).
Tonin G.A., Perez S.C.J.G.A. (2006). Qualidade fisiológica de sementes de Ocoteaporosa (Nees et Martius ex. Nees) após diferentes condições de armazenamento e semeadura, Revista Brasileira de Sementes, 28(2), 26-33. Retrieved from http://dx.doi.org/10.1590/S0101-31222006000200004. (in English).
Tsarenko O.M., Zlobin Yu.A., Sklyar V.G., Panchenko S.M. (2000). Computer methods in agriculture and biology: Textbook, University Book, 202 p. (in English).
Ushkarenko V.O. (2008). Dispersion and correlation analysis of the results of field experiments, Tutorial, 372 p. (in English)
Walters C., Lana Wheeler M., Judith M. (2007). Longevity of seeds stored in a genebank: species characteristics, Seed Science Research, 15(1), 1-20. (in English).
Zemlyanukhin A.A. (1985). Small work shop on biochemistry, Tutorial, 128 p. (in Russian).