Comparative GC-MS study of Schizonepeta multifida essential oil from Khakassia Republic shows potentials for nutraceuticals, flavor, and conservation

Abstract

A. Tkachev, N. Nekratova, N. Belousova, M. Shurupova, W. Letchamo

Schizonepeta multifida (L.) Briq. (Lamiaceae) is among the widely used botanicals in Siberian traditional and modern health and personal care practices, shelf life extension of agricultural produce, antimicrobial, and insect repellent. We investigated the variations or differences in the chemical composition of essential oils in wild accessions harvested from two distinct geographical regions, ‘habitat-1’ and ‘habitat-2’, in the Republic of Khakassia, Siberia. The oils were obtained by subjecting the aboveground parts of the plant to hydro-distillation, with subsequent assessment of the chemical composition using GC-FID and GC-MS analyses. The oil composition varied quite a lot according the number of components (61 in samples from ‘habitat-1’, 45 in sample from ‘habitat-2’),  the dominant components (pulegone 33.36 %, limonene 20.95 %, cis-beta-ocimene 9.96 % and isomenthone 5.31 % from ‘habitat-1’, limonene 27.98 %, cis-beta-ocimene 14.42 %, pulegone 14.20 %, beta-myrcene 11.95 %, and terpinolene 6.33 % from ‘habitat-2’). In light of the continued overharvesting resulting in genetic erosion, further studies are warranted to establish in-situ and ex-situ conservation strategies of all populations representing different chemotypes/ecotypes and identifying promising genetic lines for sustainable cultivation as a source of renewable raw material for the oil.

Keywords: Schizonepeta multifida, Lamiaceae, pulegone, limonene, cis-beta-ocimene, nutraceuticals
 

References

 

Adams, R.P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry. Carol Stream, USA.

Chen, X.W., Serag, E.S., Sneed, K.B., & Zhou, S.-F. (2011). Herbal bioactivation, molecular targets and the toxicity relevance. Chemico-Biological Interactions. 192(3), 161-176. https://doi.org/: 10.1016/j.cbi.2011.03.016

Choi, J.H., Lee, M.J., Jang, M., Kim, F.J., Shim, L., Kim, H.-J., Lee, S., Lee, S.W., Kim, Y.O., & Cho, I.H. (2015). An oriental medicine, Hyungbangpaedok-san attenuates motor paralysis in an experimental model of multiple sclerosis by regulating the t-cell response. PLoS ONE. 10(10), e0138592. https://doi.org/10.1371/journal.pone.0138592.

Choi, Y.Y., Kim, M.H., Jung, H.S., Sohn, Y., Choi, Y.J., Hwang, M.K., Kim, S.H., Kim, J.,  Yang, W.M. (2013). Schizonepeta tenuifolia inhibits the development of atopic dermatitis in mice. Phytotherary Research. 27(8), 1131-1135. https://doi.org/10.1002/ptr.4833.

Council of Europe (2008). European Pharmacopoeia. Determination of essential oils in herbal drugs. 2.8.12. Strasbourg.

Filippova, G.V. (2003). Role of environmental factors in the accumulation of biologically active substances of plants in Yakutia. Ph. D. Thesis, Yakutsk (in Russian).

Food and Drug Administration-DHHS (2012). Website Synthetic flavoring substances and adjuvants. http://www.gpo.gov.

Guskova, I.M (1965). Dynamics of accumulation of essential oil in Schizonepetamultifida (L.) Briq. In:  New useful plants of Siberia; introduction and acclimatization. Novosibirsk, Russia.

Isohama, Y. (2014).  Increase in aquaporin 3 expression in keratinocytes by Schizonepeta tenuifolia. Folia Pharmacologica Japonica. 143(3), 115-119. https://doi.org/10.1254/fpj.143.115.

Ivanov, S.L. (1961).  Climatic theory of the formation of organic substances. Moscow (in Russian).

Kang, H., Moon, J.Y., & Sohn, N.W. (2010). Regulation of interferon-gamma, interleukin-4 and interleukin-2 by Schizonepeta tenuifolia through differential effects on nuclear factor-kappa B, NFATc2 and STAT4/6. Experimemntal Biology and Medicine (Maywood, NJ). 235(2), 230-236. https://doi.org/10.1258/ebm.2009.008357.

Kang, H., Han, S.W., Hon, J.W., & Sohn, N.W. (2010). Suppression of tumor necrosis factor-alpha by Schizonepeta tenuifolia water extract via inhibition of I kappa b alpha degradation and jun n-terminal kinase/stress-activated protein kinase activation. The Journal of Pharmacy and Pharmacology. 62(8),1069-1076. https://doi.org/10.1111/j.2042-7158.2010.01126.x.

Korolyuk, E.A., & Tkachev, A.V. (2002). Essential oil from two species of Schizonepeta growing in the Altai Mountains. Khimija Rastitel’nogo Syr’ja.  1, 53-56.

Lee, S.O., Park, I.K., Choi, G.J., Lim, H.K., Jang, K.S., Cho, K.Y., Shin, S.C., &  Kim, J.C. (2007). Fumigant activity of essential oils and components of Illicium verum and Schizonepeta tenuifolia against Botrytis cinerea and Colletotrichum gloeosporioides. Journal of Microbiology and Biotechnology. 17(9), 1568-1572.

Letchamo, W., Korolyuk, E.A., & Tkachev, A.V. (2005). Chemical screening of essential oil bearing flora of Siberia II. Composition of the essential oil of Schizonepeta annua (Pallas) Schischkin leaves from Altai Region. Journal of Essential Oil Research. 17(3), 314-315. https://doi.org/10.1080/10412905.2005.9698915.

Li, H.W., & Hedge, I.C. (1994). Lamiaceae. In: Wu, C.Y., & Raven, P.H. (Eds.) Flora of China. Vol. 17. St. Louis, pp. 269-291.

Liu, Z.L., Chu, S.S., & Jiang, G.H. (2011). Toxicity of Schizonepeta multi?da essential oil and its constituent compounds towards two grain storage insects. Journal of the Science of Food and Agriculture. 5(91), 905-909. https://doi.org/10.1002/jsfa.4263.

Malyshev, D.I. (1997). Genus Schizonepeta Bentham. In: Edits., V.M. Doronkin et al. Flora of Siberia. Pyrolaceae–Lamiaceae (Labitae). Vol. 11. Novosibirsk (in Russian).

Myadelets, M.A., Domrachev, D.V., & Vodolazova, S.V. (2012). Analysis of a chemical composition of the essential oils of Nepeta sibirica L., Thymus petreus L. and Schizonepeta multifida L., growing in territory of the Republic of Khakassia. Khimija Rastitel’nogo Syr’ja.  4, 119–124.

Peshkova, V.A., Mironovich, V.I., & Martynov, A.M. (1985).  Researches of some plants of family Rosaceae and Lamiaceae growing in the Irkutsk oblast. In: Results and prospects of scientific studies in creation and development of medicines from plants. Moscow (in Rusian).

Plennik, R.Y., Gontar, E.M., & Tyurina, E.V. (1989). Useful plants of Khakassia. Resources and introduction. Novosibirsk (in Russian).

Polyakova, M.A., Dembicz, I., Becker, T., Becker, U., Demina, O.N., Ermakov, N., Filibeck, G., Guarino, R., Janišová, M., Jaunatre, R., Kozub, L., Steinbauer, M.J., Suzuki, K.H., & Dengler, J. (2016). Scale and taxon dependent patterns of plant diversity in steppes of Khakassia, South Siberia (Russia). Biodiversity and Conseration. 25(12), 2251-2273. https://doi.org/10.1007/s10531-016-1093-y.

Ra, J., Chung, J.-H., Lee, H., & Kim J. (2011).  Reduction of interleukin-1 beta induced matrix metalloproteinase-3 release by extracts of six plants: Inhibitory screening of 35 traditional medicinal plants. Immunopharmacology and Immunoxycology. 33(3), 461-465. https://doi.org/10.3109/08923973.2010.537663.

Rabzhaeva, A.N. (2011). Features of accumulation of Thymus baicalensis Serg. biologically active substances depending on environmental factors. Ph. D. Thesis. Ulan-Ude (in Russian).

Sanduin, S., & Altanctetceq, S. (2003). Volatile constituents of wild  growing Schizonepeta species from Mongolia. Journal of Essential Oil Bearing Plants. 6, 214-216. https://doi.org/10.1080/0972-060X.2003.10643356.

Sobolevskaya, K.A. (1972). Useful plants of the eastern Siberia and prospects of their introduction to cultivation. Novosibirsk (in Russian).

Sokolov, P.D. (1991). Plant resources of the USSR: Flowering plants, their chemical composition and use. Vol. 4. Families Hippuridaceae-Lobeliaceae. Leningrad (in Russian).

Tkachev, A.V. (2008). Investigation of volatile components of plants. Novosibirsk (in Russia).

Tkachev, A.V., Korolyuk, E.A., Yusubov, M.S., & Guriev, A.M. (2002). Changes in the composition of the essential oil in different stages of storage of raw materials.  Khimija Rastitel’nogo Syr’ja. 1, 19-30.

Wang, B.S., Huang, G.J., Tai, H.M., & Huang, M.H. (2012). Antioxidant and anti-inflammatory activities of aqueous extracts of Schizonepeta tenuifolia Briq. Food and Chemical Toxicology.  50(3-4), 526-531. https://doi.org/10.1016/j.fct.2011.12.010.

Wu, Z.-Q., Liu, G.-H., Yan, L.-J., Nan, C.-H., Yue, Z.-J., Wang, X.-F. (2010). Experimental study on anti-influenza virus infection with yinqiao-decoction by orthogonal design. Chinese Journal of Experimental and Clinical Virology. 24(6), 427-429 (in Chinese).

Yoo, J.-S., Kim, D.K., Kim, S.-H., & Shin, T.Y. (2011). Anti-allergic effects of Schizonepeta tenuifolia on mast cell-mediated allergy model. Natural Product Scence. 17, 239-244.

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