Cytogenetic characteristics of some Trollius L. species (Ranunculaceae) from Asian Russia
Abstract
E. Yu. Mitrenina, A. S. Erst, M. V. Skaptsov, T. N. Veklich, O. A. Chernysheva, M. G. Kutsev, A. A. Kuznetsov
The karyotypes (chromosome sets) of Trollius austrosibiricus, T. kytmanovii, and T. riederianus have been investigated for the first time. The karyotype formula has been obtained as 2n = 2x =16 = 2m + 2m/sm + 12sm for Trollius austrosibiricus and T. riederianus, and 2n = 2x =16 = 4m + 12sm for T. kytmanovii. A comparative analysis of the chromosome sets was conducted for these three species and the previously studied Trollius altaicus, T. asiaticus, T. ledebourii, and T. lilacinus (Hegemone lilacina) for several karyological parameters. The average absolute nuclear DNA content (2?-value) was originally determined for Trollius austrosibiricus, T. kytmanovii, T. ledebourii, and T. riederianus by flow cytometry.
Keywords: chromosomes, C-value, globe-flower, karyotype, nuclear DNA content, Ranunculaceae, Trollius L.
References
Altinordu F., Peruzzi L., Yu Y., He X. (2016) A tool for the analysis of chromosomes: KaryoType. Taxon, 65 (3), 586–592.
Badaeva E. D., Salina E. A. (2013) Genome structure and chromosomal analysis of plants. Vavilov Journal of Genetics and Breeding, 17 (4/2), 1017–1043 [In Russian].
Baltisberger M., Hörandl E. (2016) Karyotype evolution supports the molecular phylogeny in the genus Ranunculus (Ranunculaceae). Perspectives in Plant Ecology, Evolution and Systematics, 18, 1–14. DOI: 10.1016/j.ppees.2015.11.001
Butkov A. Y. (1953) Hegemone. In: Vvedensky A. I. & Korovin E. P. (eds.), Flora Uzbekistan. 2, 439. “FAN”, Taschkent (In Russian).
Czerepanov S. K. (1995) Vascular Plants of Russia and Adjacent States (the Former USSR). Cambridge University Press, Cambridge.
Del Grosso F., Pogliani M. (1971) Studio cariologico di Adonis distortus Ten. Biogeographia – The Journal of Integrative Biogeography, 2(1), 69–79. DOI: 10.21426/B62110466
Dole?el J., Sgorbati S., Lucretti S. (1992) Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiologia Plantarum, 85, 625–631. DOI: 10.1111/j.1399-3054.1992.tb04764.x
Doroszewska A. (1967) Chromosomes of some Trollius species. Acta Societatis Botanicorum Poloniae. 36(3), 567–577.
Doroszewska A. (1974) The genus Trollius L.: A taxonomical study. Warszawa: Panstwowe wydawnictwo naukowe. Monographiae Botanicae. 41.
Erst A. S., Luferov A. N., Troshkina V. I., Shaulo D. N., Kuznetsov A. A., Xiang Kunli, Wang Wei (2019) Trollius austrosibiricus (Ranunculaceae), a new species from South Siberia. PhytoKeys, 115, 83–92. DOI: 10.3897/phytokeys.115.30863
Erst A. S., Pendry C. A., Ikeda H., Wang W. (2020a) Hegemone micrantha (Ranunculaceae) – A New Record from Nepal and China. Journal of Japanese Botany, 95(5), 303–305.
Erst A. S., Sukhorukov A. P., Mitrenina E. Yu., Skaptsov M. V., Kostikova V. A., Chernisheva O. A., Troshkina V. I., Kushunina M., Krivenko D. A., Ikeda H., Xiang K., Wang W. (2020b) An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys, 140, 75–100. DOI: 10.3897/phytokeys.140.49048
Friesen N. V. (2003) Hegemone Bunge. In: Malyschev L. I. & Peschkova G. A. (eds.), Flora of Siberia. Portulacaceae–Ranunculaceae. Science Publishers, Enfield, New Hampshire, 6, 103–104.
Greilhuber J., Dole?el J., Lysák M. A., Bennett M. D. (2005) The origin, evolution and proposed stabilization of the terms ‘Genome Size’ and ‘C-Value’ to describe nuclear DNA contents. Annals of Botany, 95(1), 255–260. DOI:10.1093/aob/mci019
Hoot S. B., Meyer, K. M., Manning, J. C. (2012) Phylogeny and reclassification of Anemone (Ranunculaceae), with an emphasis on austral species. Syst. Bot, 37, 139–152. DOI: 10.1600/036364412x616729
Jiang M., Yan L., Li K., Ji Zh., Tian Sh. (2020) Evaluation of total phenol and flavonoid content and antimicrobial and antibiofilm activities of Trollius chinensis Bunge extracts on Streptococcus mutans. Microscopy, research & technique, 83(12), 1471–1479. DOI: 10.1002/jemt.23540
Ikeda H., Tsusaka M., Kaneko Sh., Kariyama Sh. (2006) A new locality of Adonis multiflora (Ranunculace??and its karyomorphology. Journal of Phytogeography and Taxonomy, 54, 65–69.
Kadota Y. (2016) A revision of the genus Trollius (Ranunculaceae) in Japan. J. Jap. Bot, 91, 178–200.
Kitagawa M. (1979) Neo-Lineamenta Florae Manshuricae – Flora et vegetatio mundi. Gantner, Vaduz.
Kubešová M., Moravcová L., Suda J., Jarošík V., Pyšek P. (2010) Naturalized plants have smaller genomes than their non-invading relatives: a flow cytometric analysis of the Czech alien flora. Preslia, 82, 81–96.
Kurita M. (1955) Cytological studies in Ranunculaceae IV. The karyotype analysis in Actaea and some other genera. Jap. Jour. Genet, 30, 124–127.
Kurita M. (1957) Chromosome studies in Ranunculaceae VI. Karyotypes of six genera. Rep. Biol. Inst. Ehime Univ, 3, 9–15.
Kurita M. (1959) Chromosome studies in Ranunculaceae XIV. Karyotypes of several genera. Mem. Ehime Univ. Sect. II. 3, 25–32.
Kurita M. (1960) Chromosome studies in Ranunculaceae XVII. Karyotypes of some species. Mem. Ehime Univ. Sect. II. 4, 59–66.
Langlet O. (1932) Über chromosomenverhältnisse und systematik der Ranunculaceae. Svensk Botanisk Tidskrift, 26, 381–400.
Lee Y. N., Yeau S. H. (1985) Taxonomic characters of Megaleranthis saniculifolia Ohwi (Ranunculaceae). Korean Journal of Plant Taxonomy, 15, 127–131.
Levan A., Fredga K., Sandberg A. (1964) Nomenclature for centrometric position of chromosomes. Hereditas, 52, 201–220.
Li L.-Q., Tamura M. (2001) Trollius Linnaeus. In: Wu Z.-Y., Raven P. H. and Hong D.-Y. (eds.). Flora of China. Science Press, Beijing and Missouri Botanical Garden Press, St. Louis, 6, 137–142.
Luferov A. N. (1991) New nomenclatural combinations in taxonomy of Far Eastern species of the family Ranunculaceae Juss. Bulletin of Moscow Society of Naturalists, Biological series, 96(5), 74–76. [In Russian]
Luferov A. N. (2004) A taxonomic synopsis of Ranunculaceae of the Far East of Russia. Turczaninowia, 7(1), 1–85. [In Russian]
Luferov A., Erst A., Luferov D., Shmakov A., Wang W. (2018) The genus Trollius (Ranunculaceae) in the Russian Far East. Turczaninowia, 21(2), 110–116. DOI: 10.14258/turczaninowia.21.2.12
Mártonfiová L. (2013) A method of standardization of chromosome length measurement. Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics, 66(4), 304–312. DOI: 10.1080/00087114.2013.854565
Mitrenina E. Yu., Erst A. S., Skaptsov M. V., Kutsev M. G., Kuznetsov A. A. (2020) Karyotypes and nuclear DNA content in some Trollius L. and Hegemone Bunge ex Ledeb. (Ranunculaceae) species of Asian Russia and China. Turczaninowia, 23(1), 90–98. DOI: 10.14258/turczaninowia.23.1.9
Mlinarec J., S??atovic Z., Mihelj D., Malenica N., Besendorfer V. (2012) Cytogenetic and phylogenetic studies of diploid and polyploid members of Tribe Anemoninae (Ranunculaceae). Plant Biology, 14, 525–536. DOI:10.1111/j.1438-8677.2011.00519.x
Paszko A. (2006) A critical review and a new proposal of karyotype asymmetry indices. Pl. Syst. Evol. 258, 39–48. DOI: 10.1007/s00606-005-0389-2
Peruzzi L., Ero??lu H. E. (2013) Karyotype asymmetry: Again, how to measure and what to measure? Comparative Cytogenetics, 7, 1–9. DOI: 10.3897/compcytogen.v7i1.4431
Rice A., Glick L., Abadi Sh., Einhorn M., Kopelman N.M., Salman-Minkov A., Mayzel J., Chay O., Mayrose I. (2015) The Chromosome Counts Database (CCDB) – a community resource of plant chromosome numbers. New Phytologist. 206(1), 19–26. http://ccdb.tau.ac.il/
Ricroch A., Yockteng R., Brown S. C., Nadot S. (2005) Evolution of genome size across some cultivated Allium species. Genome, 48, 511–520. DOI: 10.1139/g05-017
Schipczinsky N. V. (1937) Hegemone Bge. In: Komarov V. L. & Shishkin B. K. (eds.), Flora of the USSR. Publishing House of the Academy of Sciences of the USSR, Moscow, 7, 53–54. [In Russian].
Schrager L. N., Malakhova L. A. (1981) An analysis of karyotypes of Adonis vernalis and Clematis integrifolia (Ranunculaceae). Bot. Zhurn. (Moscow & Leningrad), 66(3), 438–440. [In Russian]
Serebryanyi M. M. (2019) Towards a taxonomic revision of the genus Trollius (Ranunculaceae) in the Asian part of Russia. I. Trollius chinensis: taxonomic and geographical reconsiderations. Novitates Systematicae Plantarum Vascularium, 50, 101–114. DOI: 10.31111/novitates/2019.50.101
Sharma A., Sen S. (2019) Chromosome botany. CRC Press; 1st edition.
Siplivinsky V. N. (1972) The genus Trollius L. on the North and East of Asia. Nov. Syst. Pl. Vascul. Leningrad, 9, 163–182 [In Russian with Latin descriptions].
Smirnov S., Skaptsov M., Shmakov A., Fritsch R., Friesen N. (2017) Spontaneous hybridization among Allium tulipifolium and A. robustum (Allium subg. Melanocrommyum, Amaryllidaceae) under cultivation. Phytotaxa, 303(2), 155–164. DOI: 11646/phytotaxa.303.2.5
Smirnov Yu. A. (1968) Accelerated method for studying somatic chromosomes in fruit trees. Tsitologia, 10(12), 1601–1602. [In Russian]
Sramkó G., Laczkó L., Volkova P. A., Bateman R. M., Mlinarec J. (2019) Evolutionary history of the Pasque-flowers (Pulsatilla, Ranunculaceae): Molecular phylogenetics, systematics and rDNA evolution. Mol Phylogenet Evol, 135, 45–61. DOI: 10.1016/j.ympev.2019.02.015
Stebbins G. L. (1971) Chromosomal evolution in higher plants. London: Arnold.
Volkova S. A., Gorovoy P. G., Pshennikova L. M. (2020) Karyotypes of Adonis amurensis (Ranunculaceae) in the Primorsky Territory and in Sakhalin. Turczaninowia, 23(2), 39–48. DOI: 10.14258/turczaninowia.23.2.6 [In Russian]
Voroshilov V. N. (1982) Plants of the Soviet Far East. Nauka, Moscow [In Russian]
Wang W., Hu H., Xiang X.-G., Yu Sh.-X., Chen Zh.-D. (2010) Phylogenetic placements of Calathodes and Megaleranthis (Ranunculaceae): Evidence from molecular and morphological data. Taxon, 59(6), 1712–1720. DOI: 10.2307/41059868
Witkowska-Banaszczak E. (2015) The Genus Trollius – Review of Pharmacological and Chemical Research. Phytotherapy Research, 29(4), 475-500. DOI: 10.1002/ptr.5277.
Yang Q.-E. (2001) Cytology of eleven species in the genus Ranunculus L. and five in its four related genera from China. Acta Phytotaxonomica Sinica, 39, 405–422.
Yang Q.-E. (2002) Cytology of the tribe Trollieae and of the tribe Cimicifugeae in the Ranunculaceae: a comparative study. Acta Phytotaxonomica Sinica, 40, 52–65.
Yuan Q., Yang Q.-E. (2006) Tribal relationships of Beesia, Eranthis, and seven other genera of Ranunculaceae: evidence from cytological characters. Botanical Journal of the Linnean Society, 150, 267–289.