Ecological and toxicological characteristics of selenium nanocompounds

Abstract

S.I. Tsekhmistrenko, V.S. Bityutskyy, O.S. Tsekhmistrenko, V.M. Kharchishin, N.O. Tymoshok, A.A. Demchenko, M.Ya. Spivak, I.M. Kushnir, O.I. Rozputnyy, V.M. Polishchuk, N.V. Ponomarenko, N.V. Rol, N.M. Prysiazhniuk, I.V. Pertsovyi, T.S. Tokarchuk

The toxicity of selenium and probiotic preparations by intragastric administration to laboratory animals (white rats), weight coefficients of their internal organs, hematological parameters of blood were determined. The studies were performed on three groups of white rats, which for thirty days received sodium selenite with probiotics and bionanoselenium at doses of fed drugs 1000, 3000, and 5000 mg/kg. We considered physiological parameters, namely, appearance, condition of fur and visible mucous membranes, behavior, rhythm, respiratory rate, attitude to food, time of onset and nature of intoxication, severity, course time of death, or recovery. Studies have shown the absence of death of animals or diseases with intragastric use of the studied drugs, which allows them to be classified as toxicity class 4 – low-toxic substances. At autopsy of the internal organs of the thoracic and abdominal cavities, no pathological changes were detected, and no significant changes in the weight of the liver, heart, lungs, and kidneys, compared with the control group, for 30 days of study supplemented with a simultaneous increase in spleen weight. No signs of inflammation, circulatory disorders, and trophies were found in the parenchymal organs. Long-term admission of the studied drugs caused a probable increase in hematological parameters (hemoglobin concentration, number of erythrocytes and leukocytes, hematocrit) in the experimental animals. 30-day application of nanoselenium caused an increase in total protein content in animals of the experimental groups by 7.9–11.5% (p <0.05) compared with the control group. There was a probable increase (p <0.01) in urea levels. The use of therapeutic, 5-fold and 10-fold therapeutic doses caused an increase in the content of total cholesterol and medium-weight molecules compared to the control group. The use of nanoselen did not cause significant changes in transaminase activity and glucose levels. The conducted primary toxicological study is the basis for developing and using low-toxic, effective, and environmentally friendly drugs for industrial poultry and livestock that do not cause side effects.

Keywords: nanopreparations, nanoselenium, sodium selenite, white rats, hematological parameters, blood biochemical parameters
 

References

 

Alagawany, M., El-Saadony, M.T., Elnesr, S.S., Farahat, M., Attia, G., Mahmoud, M., ... & Reda, F.M. (2021). Use of Chemical Nano Selenium as Antibacterial and Antifungal Agent in Quail Diets and its Effect on Growth, Carcasses, Antioxidant, Immunity and Caecal Microbe. Research Square, 28, https://doi.org/10.21203/rs.3.rs-495839/v1

Arnaut, P.R., da Silva Viana, G., da Fonseca, L., Alves, W.J., Muniz, J.C.L., Pettigrew, J.E., ... & Hannas, M.I. (2021). Selenium source and level on performance, selenium retention and biochemical responses of young broiler chicks. BMC Veterinary Research, 17(1), 1–13. https://doi.org/10.1186/s12917-021-02855-4

Bityutsky, V.S., Tsekhmistrenko, S.I., Tsekhmistrenko, ?.S., Tymoshok, N.O., & Spivak, M.Y. (2020). Regulation of redox processes in biological systems with the participation of the Keap1/Nrf2/ARE signaling pathway, biogenic selenium nanoparticles as Nrf2 activators. Regulatory Mechanisms in Biosystems, 11(4), 483–493. https://doi.org/10.15421/022074

Boostani, A., Sadeghi, A.A., Mousavi, S.N., M. Kashan, N. (2015). Effects of organic, inorganic, and nano-Se on growth performance, antioxidant capacity, cellular and humoral immune responses in broiler chickens exposed to oxidative stress. Livestock science, 178, 330–336. https://doi.org/10.1016/j.livsci.2015.05.004

Dalia, A.M., Loh, T.C., Sazili, A.Q., & Samsudin, A.A. (2020). Influence of bacterial organic selenium on blood parameters, immune response, selenium retention and intestinal morphology of broiler chickens. BMC Veterinary Research, 16(1), 1–10. https://doi.org/10.1186/s12917-020-02587-x

El-kazaz, S.E., Abo-Samaha, M.I., Hafez, M.H., El-Shobokshy, S.A., & Wirtu, G. (2020). Dietary supplementation of nano-selenium improves reproductive performance, sexual behavior and deposition of selenium in the testis and ovary of Japanese quail. Journal of advanced veterinary and animal research, 7(4), 597–607. Doi: 10.5455 / javar.2020.g457

Israel, L.L., Braubach, O., Galstyan, A., Chiechi, A., Shatalova, E.S., Grodzinski, Z., Ding, H., Black, K.L., Ljubimova, J.Y., & Holler, E. (2019). A combination of tri-Leucine and angiopep-2 drives a polyanionic polymalic acid nanodrug platform across the blood–brain barrier. ACS nano, 13(2), 1253–1271. https://doi.org/10.1021/acsnano.8b06437

Kaphle, A., Navya, P.N., Umapathi, A., & Daima, H.K. (2018). Nanomaterials for agriculture, food and environment: applications, toxicity and regulation. Environmental Chemistry Letters, 16(1), 43–58. https://doi.org/10.1007/s10311-017-0662-y

Misra, B.B., Langefeld, C., Olivier, M., & Cox, L.A. (2019). Integrated omics: tools, advances and future approaches. Journal of molecular endocrinology, 62(1), R21–R45. https://doi.org/10.1530/JME-18-0055 

Panieri, E., Telkoparan-Akillilar, P., Suzen, S., & Saso, L. (2020). The NRF2/KEAP1 Axis in the Regulation of Tumor Metabolism: Mechanisms and Therapeutic Perspectives. Biomolecules, 10(5), 791. https://doi.org/10.3390/biom10050791

SOU 85.2-37-736:2011. Preparaty veterynarni. Vyznachannia hostroi toksychnosti. K: Minahropolityky, 2011, 16 ?. (in Ukrainian).

Staurengo-Ferrari, L., Badaro-Garcia, S., Hohmann, M. S., Manchope, M. F., Zaninelli, T. H., Casagrande, R., & Verri, Jr. W. A. (2019). Contribution of Nrf2 modulation to the mechanism of action of analgesic and anti-inflammatory drugs in pre-clinical and clinical stages. Frontiers in pharmacology, 9, 1536. https://doi.org/10.3389/fphar.2018.01536

Surai, P.F., Kochish, I.I. Velichko, O.A. (2017). Nano-Se Assimilation and Action in Poultry and Other Monogastric Animals: Is Gut Microbiota an Answer? Nanoscale research letters, 12(1), 612. https://doi.org/10.1186/s11671-017-2383-3

Tsekhmistrenko, O.S., Bityutskyy, V.S., Tsekhmistrenko, S.I., Kharchishin, V.M., Melnichenko, O.M., Rozputnyy, O.I., ... & Onyshchenko, L.S. (2020a). Nanotechnologies and environment: A review of pros and cons. Ukrainian Journal of Ecology, 10(3), 162–172. doi: https://doi.org/10.15421/2020_149  

Tsekhmistrenko, S.I., Bityutskyy, V.S., Tsekhmistrenko, O.S., Horalskyi, L.P., Tymoshok, N.O., & Spivak, M.Y. (2020b). Bacterial synthesis of nanoparticles: A green approach. Biosystems Diversity, 28(1), 9–17. https://doi.org/10.15421/012002

Tsekhmistrenko, S.I., Bityutskyy, V.S., Tsekhmistrenko, O.S., Melnichenko, O.M., Kharchyshyn, V.M., Tymoshok, N.O., Ponomarenko, N.V., Polishchuk, S.A., Rol, N.V., Fedorchenko, M.M., Melnichenko, Yu.O., Merzlova, H.V., Shulko, O.P., Demchenko, A.A. (2020c). Effects of selenium compounds and toxicant action on oxidative biomarkers in quails. Ukrainian Journal of Ecology, 10(2), 232–239. doi: https://doi.org/10.15421/2020_89  

Tu, W., Wang, H., Li, S., Liu, Q., & Sha, H. (2019). The Anti-Inflammatory and Antioxidant Mechanisms of the Keap1/Nrf2/ARE Signaling Pathway in Chronic Diseases. Aging and disease, 10(3), 637–651. doi: 10.14336 / AD.2018.0513

Tymoshok, N.O., Kharchuk, M.S., Kaplunenko, V.G., Bityutskyy, V.S., Tsekhmistrenko, S.I., Tsekhmistrenko, O.S., Spivak, M.Y., & Melnichenko, ?.?. (2019). Evaluation of effects of selenium nanoparticles on Bacillus subtilis. Regulatory Mechanisms in Biosystems, 10(4), 544–552. https://doi.org/10.15421/021980

Yang, J., Wang, J., Huang, K., Liu, Q., Xu, X., Liu, G., ... & Zhu, M. (2021). Selenium Enriched Bacillus Subtilis Yb-1114246 Improved Growth and Immunity of Broiler Chickens Through Modified Ileal Bacterial Composition. Research Square, https://doi.org/10.21203/rs.3.rs-410082/v1  

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