I. Kurbatova*, M. Zakharenko, O. Tupitska, O. Yaremchuk and L. Chepil

We assumed that effect of sulfanilamide, chlortetracycline, nandrolone, and albendazole on the carp fishes depends on substance concentration in the water and associated with changes in the fractional composition of plasma protein. We found that sulfanilamide in concentration of 0.015 mg/dm3 in water practically had no effect, while at concentrations of 0.15 and 0.30 mg/dm3 it changed the protein spectrum of carp blood plasma, reduced the level of proteins with molecular weights of 260 and 140 kDa. Chlortetracycline in the concentration of 1.10 mg/dm3 did not affect the protein content of macromolecular fractions in the blood plasma of fish, and at the doses of 3.15 and 6.30 mg/dm3 it reduced the content of proteins with molecular weights of 450, 340, 260 and 140 kDa. Nandrolone in the concentration of 1.10 mg/dm3 increased the content of individual proteins in carp plasma compared to the control, and in the concentration of 0.10 and 0.50 mg/dm3 it did not affect the fractional composition of proteins with high molecular weight. Albendazole in the concentrations of 0.2, 0.5, and 1.00 mg/dm2 reduced the protein content of macromolecular fractions in carp plasma. Such xenobiotics, like sulfanilamide, chlortetracycline, and albendazole in different concentrations did not affect the total protein content of fish plasma, while the nandrolone increased it. The number of respiratory movements, behavior, body surface, and pathomorphological parameters of the main internal organs in the fish from the experimental groups did not differ from the control. Our results indicated the important role of proteins of high molecular weight fractions in carp blood plasm as these proteins determine the fish adaptation to the water xenobiotics.

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