Effect of application of modified nourishing environment on the reproduction and yielding capacity of root celery
T.V. Polischuk, O.I. Ulianich, V.V. Polischuk, V.V. Ketskalo, N.V. Vorobiova
We set the need of additional application of phytohormone at reproduction of root celery of Anita and Tsilitel varieties in vitro conditions using traditional nutrient medium Murasige-Scuga from our research within 2015–2017. For the control, the nutrient medium Murasige-Scuga (MS) was selected. To obtain a genetically identical material, the composition of nutrient medium with the concentration of plants growth regulator benzylaminopurine (BAP) 0.2 %, 0.3, and 0.5 % was investigated. We observed that prior the plants bedding-out from culture dishes into cassette, the Tsilitel variety have formed grater amount of callus tissue in comparison with Anita variety. We also established that application of Murasige-Scuga+6-BAP 0.2 % nutrient medium contribute the better growth of cultural based plants and seedlings, increasing the amount of leaves and plants height, which in its turn significantly increase the yield of multiplication material. An increase in 6-BAP 0.3 % concentration resulted in a significant reduction of these parameters, and an increase of up to 0.5 % did not contribute to plant growth. After planting the cassette seedlings into the open ground, the growth of plants at the first stages was slow, and as their adaption to the growing conditions, it was accelerated. In 30 days after plantation the biometric parameters of plants were higher due to their cultivation with addition of 6-BAP 0.2 to the nutrient medium. A similar trend was observed even in 60 days after sowing seedlings in the open ground. The researches have shown that the grater yield of studied varieties and the higher quality characteristics of their production ensured cultivation of explants at Murasige-Scuga+6-BAP 0.2 % medium.