Effect of Butaselmevit-Plus on the immune system of piglets during and after weaning


T. V. Martyshuk, B. V. Gutyj, M. M. Zhelavskyi, S. V. Midyk, A. M. Fedorchenko, V. B. Todoriuk, T. B. Nahirniak, Ya. V. Kisera, H. V. Sus, V. A. Chemerys, N. D. Levkivska, I. I. Iglitskej

The aim of our research was to study the effect of Butaselmevit-Plus feed additive on the immune system indexes of piglets during weaning. The study was conducted at Koshet LLC in Mukachevo Raion of Zakarpattia Oblast. We formed two groups of piglets: control (С) and treatment (T) with 10 individuals in each group selected on the basis of analogues – age, breed, and body weight. The animals received nutrition according to the norms for pigs of this age. On the 28th day after birth, the piglets were separated from the saw. Piglets from different litters were rearranged for further wean-to finish maintenance with changes in diet. This period is stressful for the animals. From the age of 5 days, piglets of all groups were fed with pre-starter feed. From the age of 21 to 40 days, piglets of the experimental group received additionally Butaselmevit plus at a dose of 100 mg/kg of body weight per day. Our research material was the blood collected in the morning before feeding. The blood was taken by cranial vena cava puncture – on the 20th day of life (period before weaning), on the 25th day of life (period before weaning), on the 30th day of life (2nd day after weaning), on the 35th day of life (7th day after weaning), and on the 40th day of life (12th day after weaning). Butaselmevit-Plus feed additive contributes to the increase of the number of T and B-lymphocytes in blood of piglets. Components of Butaselmevit-Plus promote activation of the cellular immune system of piglets before and after weaning. Introduced additionally, Butaselmevit-Plus also boosts functional activity of immune competent cells in piglets after weaning. This happens in the result of the redistribution of T and B-lymphocytes receptor apparatus that increases the lymphocytes avidity. When feeding Butaselmevit-Plus to piglets during weaning, we observed the increase in humoral component levels of the immune system during the whole period of study. On the 35th day of life, the treatment group of piglets with Butaselmevit-Plus showed 4.26% increase in neutrophil phagocytic activity. We also observed an increase in phagocyte number and phagocytic index of the pigs in the treatment group – on the 35th day of the experiment the indexes of treatment group exceeded the indexes of control group by 7.5% and 11.2% correspondingly.

Keywords: Pigs; Stress; Butaselmevit-Plus; Feed additive; Immune system


Ahmad, M.K., Amani, S., & Mahmood, R. (2011). Potassium bromate causes cell lysis and induces oxidative stress in humanerythrocytes. Environmental Toxicology, 29(2), 138–145. doi: 10.1002/tox.20780.

Chala, I.V., & Rusak, V.S. (2016). Redox–potential and the state of peroxide oxidation of blood lipids in cows kept under ecologically unfavorable conditions. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 2(66), 197–201. doi: 10.15421/nvlvet6640.

Cherkashina, D.V., & Petrenko, A.Y. (2006). Hepatoprotective effect of fetal tissue cytosol and its thermostable fraction in rats with carbon tetrachloride-induced hepatitis. B. Exp. Biol. Med., 141(4), 544–547. doi: 10.1007/s10517-006-0216-y.

Danchuk, V, Trach, V, Midyk, S, Danchuk, О, Ushkalov, V, & Kepple, O. (2019). The fatty acids content in the liver of japanese quails after the chemical treatment of hatching eggs. Foodscienceandtechnology, 13(2), 58-64. doi: 10.15673/fst.v13i2.1406

Gutyj, B. Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug Аmprolinsyl. Regul. Mech. Biosyst., 8(1), 41–45

Gutyj, B. V., Ostapyuk, A. Y., Sobolev, O. I., Vishchur, V. J., Gubash, O. P.,… Hrymak, K. (2019). Cadmium burden impact on morphological and biochemical blood indicators of poultry. Ukrainian Journal of Ecology, 9(1), 236-239

Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., ... Katsaraba, O. (2017). The impact of endogenous intoxication on biochemical indicators of blood of pregnant cows. Regulatory Mechanisms in Biosystems, 8(3), 438–443. doi: 10.15421/021768

Gutyj, B., Grymak, Y., Hunchak, V., Mysak, A., Nazaruk, N., … Kaplaushenko, A. (2018). Preclinical searches of the preparation Thireomagnile. Ukrainian Journal of Ecology, 8(1), 688–695. doi: 10.15421/2018_267

Gutyj, B., Leskiv, K., Shcherbatyy, A., Pritsak, V., Fedorovych, V., … Kolomiiets, I. (2017). The influence of Metisevit on biochemical and morphological indicators of blood of piglets under nitrate loading. Regulatory Mechanisms in Biosystems, 8(3), 427–432. doi: 10.15421/021766

Gutyj, B., Martyshchuk, T., Bushueva, I., Semeniv, B., Parchenko, V., … Murska, S. (2017). Morphological and biochemical indicators of blood of rats poisoned by carbon tetrachloride and subject to action of liposomal preparation. Regulatory Mechanisms in Biosystems, 8(2), 304–309. doi: 10.15421/021748.

Gutyj, B., Nazaruk, N., Levkivska, A., Shcherbatyj, A., Sobolev, A., … Guta, Z. (2017). The influence of nitrate and cadmium load on protein and nitric metabolism in young cattle. Ukrainian Journal of Ecology, 7(2), 9–13

Gutyj, B., Stybel, V., Hariv, I., Maksymovych, I., Buczek, K., … Samura, T. (2019). Influence Of Amprolinsile And Brovitacoccid On The Protein Synthesizing Function Of The Liver And Enzyme Activity In Turkey Blood Serum During Eimeria Invasion. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 10(2), 723−729.

Holovakha, V. I., Piddubnуak, О. V., Bakhur, T. I., Vovkotrub, N. V., Antipov, A. A., … Macynovich, A. O. (2018). Changes in erythrocytopoesis indices in dogs with babesiosis. Regulatory Mechanisms in Biosystems, 9(3), 379–383. doi:10.15421/021856

Jankowski, K., Jankowska, J., Ciepiela, G. A., Sosnowski, J., Wiśniewska-Kadzajan, B., & Matsyura, A. (2014). The initial growth and development of poa pratensis under the allelopathic influence of taraxacum officinale. Journal of Ecological Engineering, 15(2), 93-99. doi:10.12911/22998993.1094984

Khariv, I., Gutyj, B., Hunchak, V., Slobodyuk, N., Vynyarska, A., … Seniv, R. (2017). The influence of brovitatoxide in conjunction with milk thistle fruits on the immune system of turkeys for eimeriozic invasion. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(73), 163–168. doi: 10.15421/nvlvet7334

Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematological indices of rat organisms under conditions of oxidative stress and liposomal preparation action. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(1), 276–289. doi: 10.15421/201615.

Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I.,… Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541. doi: 10.15421/2017_157.

Kotsumbas, H.I., & Hryniv, М.І. (2016). The influence of feed additives on productivity hematological and immunological parameters of the broiler chicks blood. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 3(70), 157–160. doi:10.15421/nvlvet7037.

Kozak, V. M., & Brygadyrenko, V. V. (2018). Impact of cadmium and lead on Megaphyllum kievense (Diplopoda, Julidae) in a laboratory experiment. Biosystems Diversity, 26(2), 128–131. doi: 10.15421/011820.

Kulyaba, O., Stybel, V., Gutyj, B., Turko, I., Peleno, R., … Oseredchuk, R. (2019). Effect of experimental fascioliasis on the protein synthesis function of cow liver. Ukrainian Journal of Ecology, 9(4), 612-615

Kushnir, I. M., Kushnir, V. I., Gufriy D. F., Gutyj, B. V., Vishchur, V. Ya., … Stoyanovskyy, V. G. (2019). Subacute toxicity of the preparation "Biovir-P". Research Journal of Pharmaceutical, Biological and Chemical Sciences, 10(2), 674−680.

Lieshchova, M. A., Brygadyrenko, V. V., Tishkina, N. M., Gavrilin, P. M., & Bohomaz, A. A. (2019). Impact of polyvinyl chloride, polystyrene, and polyethylene on the organism of mice. Regulatory Mechanisms in Biosystems, 10(1), 50–55. doi: 10.15421/021908.

Lieshchova, M. A., Tishkina, N. M., Bohomaz, A. A., Gavrilin, P. M., & Brygadyrenko, V. V. (2018). Combined effect of glyphosphate, saccharin and sodium benzoate on rats. Regulatory Mechanisms in Biosystems, 9(4), 591–597. doi: 10.15421/021888.

Magomedaliev, I.M., Nekrasov, R.V., Chabaev, M.G., Dzhavakhiya, V.V., Glagoleva, E.V., ... Matsyura, A. V. (2019). Use of different concentrations of enzymesporin probiotic in feeding of growing young pigs. Ukrainian Journal of Ecology, 9(4), 705-708. Doi: 10.15421/2019_152

Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of ‘‘Butaselmevit’’. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (2), 22–27. doi: 10.15421/201631.

Petrukh, I.M., Simonov, M.R., & Vlizlo, V.V. (2015). Mineralnyi homeostaz u koriv, khvorykh na ketoz, za likuvanni preparatom “Remivital”. Naukovyi visnyk LNU veterynarnoi medytsyny ta biotekhnolohii. Seriia: Veterynarni nauky, 17(2), 183−188. (in Ukrainian).

Saba, A.B., Oyagbemi, A.A., & Azeez, O.I. (2010). Amelioration of carbon tetrachloride-induced hepatotoxicity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats. Nig. J. Physiol. Sci., 25(2), 139–147.

Skrypnyk, I.M. (2007). Gepatoprotetorni zasoby` v suchasnij gepatologiyi. Consilium Medicumllkraina, 1(5), 11–15 (in Ukrainian).

Slivinska, L.G., Shcherbatyy, A.R., Lukashchuk, B.O., Zinko, H.O., Gutyj, B.V., … Мusiienko, O.V. (2019). Correction of indicators of erythrocytopoesis and microelement blood levels in cows under conditions of technogenic pollution. Ukrainian Journal of Ecology, 9(2), 127-135

Sobolev, А., Gutyj, B., Grynevych, N., Bilkevych, V., & Mashkin, Y. (2017). Enrichment of meat products with selenium by its introduction to mixed feed compounds for birds. Regulatory Mechanisms in Biosystems, 8(3), 417-422. doi: 10.15421/021764.

Stojanovskyj, V., & Ogrodnyk, M. (2016). Function of intestinal immune barrier of piglets under technological stress. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 18(3(71), 112–116. doi: 10.15421/nvlvet7126.

Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhen u biologiyi, tvarynnycztvi ta veterynarnij medycyni. Lviv: Spolom (in Ukrainian).

Yevstafieva, V. А., Kravchenko, S. O., Gutyj, B. V., Melnychuk, V. V., Kovalenko, P. N., & Volovyk, L. B. (2019). Morphobiological analysis of Trichuris vulpis (Nematoda, Trichuridae), obtained from domestic dogs. Regulatory Mechanisms in Biosystems, 10(2), 165-171. doi: 10.15421/021924.

Zhukova, I. O., Svitlychna-Kulak, Yu. S., & Longus, N. I. (2016). Correction of stateof antioxidant protection in dogs when poisoned byneoverm. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 3(70), 95-99. doi: 10.15421/nvlvet7022.

Share this article