Effect Of Chlorotetracycline, Nandrolone, And Albendazole On Fractional Composition Of Carp Serum Proteins


I. M. Kurbatova, M. O. Zakharenko, L. V. Chepil

The influence of xenobiotics, in particular, the antibiotic chlortetracycline, anabolic steroid nandrolone and anthelmintic albendazole, on carp fish depends on their concentration in water and is associated with a change in a number of morphological indices and physiological and biochemical mechanisms in the process of their adaptation to the action of xenobiotics of water, and as shown by the study - total content and fractional composition of blood plasma proteins. In model experiments carried out on the bogs of carp, the main objective of which was to investigate the influence of xenobiotics of anthropogenic origin on the fractional composition of plasma proteins, it was established that for a short exposure (72 hours) and low concentrations of nandrolone 0.1 mg / dm3 (the first experimental group) and (0.5 mg / dm3 second experimental group), only slight changes in the protein content with a molecular weight of 25, 35-50 and 100-140 kDa in the water of the aquariums are observed at constant values of the parameters of other fractions. With an increase in the concentration of nandrolone in aquarium water up to 1.0 mg / dm3, the protein content with a molecular weight of 450 kDa and above increased by 91%, 340 kDa by 78%, 260 kDa by 101%, 70 kDa by 149%, 50 kDa - 111%, 25-50 kDa - 35-62% compared to control. Consequently, anabolic steroids, getting into the water at low concentrations do not affect, and in high stimulate biosynthesis processes of proteins in tissues, two decades of carp. The intolerance (12 hours) of carps in an aquarium with an anthelmintic concentration of albendazole in water of 0.2 mg / L varied only with individual fractions of blood plasma proteins of fish. The increase in the concentration of albendazole in water to 0.5 mg / L and especially to 1.0 mg / L affects the protein spectrum of blood plasma fish to a large extent, reducing the content of most proteins of low and high molecular weight fractions. It was found that chlortetracycline in the concentration of 1.10 mg / dm3 did not affect, and when it increased in water to 3.15 and 6.30 mg / dm3, it reduced the level of proteins in the blood plasma of the carp in 3 days, with a molecular weight of 340-450 kDa, and higher and contributed to the appearance of protein fractions with a molecular weight of 140 - 200 and 70 - 90 kDa. Investigated xenobiotics chlortetracycline, nandrolone and albendazole, in the above-mentioned concentrations in water and short-term exposure of fish, did not affect their behavior, the number of respiratory movements and the tomographic indices of the internal organs. The state of the external body covers, as well as the organoleptic indices of the internal organs of fish of experimental groups for the effects of various concentrations of the studied xenobiotics, did not differ from the similar characteristics of the control group carps. The obtained results testify to the important role of blood plasma proteins in the mechanisms of their adaptation to the action of xenobiotics of water, in particular anabolic steroid nandrolone, antibiotic chlortetracycline and anthelmintic albendazole.


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