Effects of 5 SNPs on daughters’ milk performance traits produced by Ukrainian dairy sires

Abstract

Olena Fedota, Nina Lysenko, Larysa Mitiohlo, Sergiy Ruban

The aim of the study was to estimate the effects of the CAPN316, CAST282, GH L127V, GHR F279Y and A257G on milk yield, fat and protein yield and content on daughters produced by dairy sires. 24 dairy sires were 100% Holstein (14), 100% Brown Swiss (5) and Holstein mixed with another dairy breeds (5). Analyses included data on 32733 daughters for 2014-2017 years. Molecular genetic analysis was performed by PCR-RFLP. Statistical analysis revealed that population was on Hardy-Weinberg equilibrium. SNPs F279Y and A257G were not in linkage disequilibrium (r2 = 0.029, D’ = 0.074), because linkage of certain alleles was observed only in 7.4% of cases (D’). C-allele of SNP CAPN316 was strongly associated with protein content, despite C-allele of SNP CAST282 showed negative association with milk, fat and protein yield. Daughter milk performance traits (DMPTs) for SNP CAPN316 corresponded to CC>CG>GG pattern, except variations in milk and fat yield (kg). For SNP CAST282 Milk, fat and protein yield (kg) were significantly higher for CG than for CC genotype. C-allele for SNP L127V showed significant association and differences between genotypes with DYDs for fat yield (kg) – 20.45±5.53 (P<0.01) and content (%) – 0.13±0.06 (P<0.05), T-allele for SNP F279V was not significantly associated with any trait studied and A-allele for SNP A257G significantly associated with milk and fat yield (kg) for DMPTs/DYDs 1988.33±419.93/-269.69±89.92 (P<0.01) and 84.30±15.64/-8.33±3.87 (P<0.05). Results of this study reveal and support known advantages of SNPs studied in panels and selection programs.

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