Evaluation of Ultra-High-Performance Liquid Chromatography (HPLC) Tandem Mass Spectrometry for Determination of Avermectin Residues in Milk

Abstract

O. V. Bayer, O. V. Kaminska, O. V. Bondarets, O. S. Yaremchuk, O. I. Skoromna, S. V. Midyk, L. V. Shevchenko, V. M. Mykhalska, O. M. Stupak, N. V. Liniichuk

The study was conducted to evaluate the applicability of ultra high-performance liquid chromatography – tandem mass spectrometry method (UHPLC-MS/MS), establish the MS/MS detection parameters and determine the validation characteristics for the analysis of residual content of аvermectins in milk. The UHPLC-MS/MS method has proved to be accurate, practical and universal. This was confirmed by Decision limit (CCα) data: abamectin – 12.56 μg/kg, doramectin – 17.74 μg/kg, eprinomectin – 24.02 μg/kg, ivermectin – 12.53 μg/kg, moxidectin – 44.69 μg/kg, recovery is 88.7 – 110%. The data obtained for assessing the suitability, accuracy and reproducibility of the results meet the requirements of the European Directive (2002/657/EC). The efficient ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method that was developed and adopted for routine use by the laboratories of veterinary medicine, allows to detect residual quantities of 5 avermectins used in animal breeding for the prevention of helminthiases in food products including milk.

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