Genotyping method (MLVA) of pathogenic leptospires for monitoring their distribution in ecosystems


T.Ð?. Tsarenko, V.V. Ukhovskyi, L.E. Korniienko, N.M. Sakhniuk, V.U. Kassich, A.P. Palii

Leptospirosis is an important problem in veterinary medicine in Ukraine and around the world. The risk of human disease and damage from leptospirosis to livestock make it necessary to study it. For epizootic monitoring of the spread of leptospirosis, it is expedient to use molecular methods for typing pathogenic Leptospiros. In the Bila Tserkva National Agrarian University and the Institute of Veterinary Medicine of the National Academy of Sciences of Ukraine, the molecular method of genotyping of pathogenic leptospiros MLVA-VNTR was tested. The genotyping of reference strains of Leptospiros has been carried out and the genotype of the field strain isolated from the vulgar field mouse from the Ternopol region of Ukraine has been determined. The prospects of using the method for monitoring the distribution of various genotypes of leptospira in ecosystems and the potential use of the method for determining the etiological structure of leptospirosis of farm and domestic animals in Ukraine are shown. For the first time, genotyping of control strains of pathogenic leptospires from the Reference Center of Leptospirosis of the Institute of Veterinary Medicine of the NAAS in Kiev and the correspondence of the serological profiles of the strains of pathogenic leptospiros: Serogroup Canicola, Serovar Canicola Strain Hond Utrecht IV and Serogroup Icterohaemorrhagiae Serovar copenhageni Strain M20 (Fiocruz L1-130) genetic profiles of MLVA. For the first time, the field isolates obtained from wild mice in Ukraine was genotyped in MLVA.

Keywords: Leptospira; genotyping; MLVA; VNTR; surveillance; ecosystems

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