Growth response of Tabernaemontana pandacaqui Poir. in soil from a former mining site in Marinduque, Philippines

Abstract

A.J.D. Belarmino, N.M. Pampolina, E.E. Coracero

Potential hazards and immediate rehabilitation of inactive/abandoned mines are concerns in Mogpog, Marinduque. Efforts have been initiated in the area through the use of species such as Acacia auriculiformis Benth. The study focused on assessing and mass-producing endomycorrhizal fungi from plants collected in the area and investigating the growth response of indigenous Tabernaemontana pandacaqui Poir. with A. auriculiformis, endomycorrhizas, and biofertilizers in soil from mine tailings. Soil and plant collection was done in three major ecosystems: agroforestry, A. auriculiformis stand, and natural forest. Associated endomycorrhizas were isolated and prepared as an inoculant to test the response of T. pandacaqui. Field endomycorrhizal spores from T. pandacaqui were: Acauslospora (50), Glomus (16), and Gigaspora (2), which sporulated (422/75g dry soil) as inoculant. Height and diameter of T. pandacaqui were significantly (p < 0.01) better with A. auriculiformis, possibly due to its N-fixing property, though host mortality was higher brought by competition. Soil inoculants promoted the growth of T. pandacaqui but MykoVAM performed significantly (p < 0.05) in height than control. To facilitate the ecological succession in old mining, the A. auriculiformis could be maintained as a nurse crop for threatened species like T. pandacaqui and consider the potential of local endomycorrhizal isolates encouraging growth and survival.

Keywords: mining site, mycorrhiza, Acacia auriculiformis, Tabernaemontana pandacaqui, growth response
 

References

Aggangan, N. S., & Cortes, A. (2018). Screening mined-out indigenous mycorrhizal fungi for the rehabilitation of mine tailing areas in the Philippines. Reforesta, 6, 71-85.

Aggangan, N., Pampolina, N., Cadiz, N., & Raymundo, A. (2015). Assessment of plant diversity and associated mycorrhizal fungi in the mined-out sites of Atlas mines in Toledo city, Cebu for bioremediation. Journal of Environmental Science and Management, 18(1).

Brundrett M., Bougher N., Dell B., Grove T. and Malajczuk N. (1996). Working with Mycorrhizas in Forestry and Agriculture. AClAR Monograph 32. 374 + x p

Calibo, R. (n.d.). Bio-N mixing plant now operates in Lazi.

Clark, R. (1997). Arbuscular mycorrhizal adaptation, spore germination, root colonization,and host plant growth and mineral acquisition at low pH. Plant and Soil, 192(1), 15-22. doi: https://doi.org/10.1023/A:1004218915413.

Contu, S. (2012). Acacia auriculiformis. The IUCN Red List of Threatened Species: https://www.iucnredlist.org/species/19891902/19997222#habitat-ecology.

Coracero, E. & Malabrigo, P. (2020). Carbon storage potential of the tree species along the ultramafic forest in Sitio Dicasalarin, Barangay Zabali, Baler, Aurora, Philippines. AIMS Environmental Science, 7(6), 589-601. https://doi.org/10.3934/environsci.2020037.

Cunningham, S. D., & Ow, D. W. (1996). Promises and prospects of phytoremediation. Plant physiology, 110(3), 715-719.

Dela Cruz, R. (2012). Mykovam: Effective growth enhancer for coconut. Department of Agriculture-Bureau of Agricultural Research (BAR) Chronicle, 13(2).

DENR. (2014). Project Completion Report Annex C: Remediation of Risk. Philippines: National Program Support for Environment and Natural Resources Management Project.

Duponnois, R., & Planchette, C. (2003). A mycorrhiza helper bacterium enhances ectomycorrhizal and endomycorrhizal symbiosis of Australian Acacia species. Mycorrhiza, 13(2), 81-95.

Felix, R. (2004, February 10). DENR eyes rehabilitation of 22 abandoned mines. PhilStar Global: https://www.philstar.com/business/2004/02/10/238345/denr-eyes-rehabilitation-22-abandoned-mines.

Gregory, C. (2004). Marcropper in the Philippines. http://www.umich.edu/~snre492/Jones/marcopper.htm.

Greipsson, S. (2011). Phyotremediation. Nature Education Knowledge, 3(10), 7.

Leghari, S. J., Wahocho, N. A., Laghari, G. M., Laghari, A. H., Bhabhan, G. M., Talpur, K. H., Lashari, A. A. (2016). Role of Nitrogen for Plant Growth and Development: A review. Advances in Environmental Biology, 10(9), 209-219.

Lovelock, C., Andersen, K., & Morton, J. (2003). Arbuscular mycorrhizal communities in tropical forests are affected by host tree species and environment. Oecologia, 135(2), 268-279. doi: 10.1007/s00442-002-1166-3.

Medianista, R., & Labay, P. (2017). Phytosuccession and Phytosociology of Plants in Ino-Capayang Mined-out Area for Possible Phytoremediation Activities in Marinduque. 4th International Conference on Civil, Environment and Waste Management, (pp. 235-239). Manila.

Orwa, C., Mutua, A., Kindt, R., Jamnadass, R., & Anthony, S. (2009). Agroforestree Database:a tree reference and selection guide version 4.0. World Agroforestry: http://www.worldagroforestry.org/treedb/AFTPDFS/Acacia_auriculiformis.PDF.

Plumlee, G. S., Morton, R. A., Boyle, T. P., Medlin, J. H., & Centeno, J. A. (2000). An Overview of Mining-Related Environmental and Human Health Issues, Marinduque Island, Philippines: Observations from a Joint U.S. Geological Survey – Armed Forces Institute of Pathology Reconnaissance Field Evaluation, May 12-19, 2000. U. S. Geological Survey.

Raymundo, A., Aggangan, N., & Pampolina, N. (2006). Bioremediation: can plants and microbes clean up the environment? 2006 FORESPI Symposium: Forest Landscape Restoration and Rehabilitation. Los Baños, Laguna.

Sarian, Z. (2017). Trees Grow Again in Mine-out Fields. http://agriculture.com.ph/2017/01/07/trees-grow-again-in-mined-outfields/.

Schmerbeck, J., & Naudiyal, N. (2014). Acacia auriculiformis. In: Enzyklopädie der Holzgewächse: Handbuch und Atlas der Dendrologie, (pp. 1-12). doi:10.1002/9783527678518.ehg2014002.

Tajer, A. (2016). What’s the function of Phosphorus (P) in plants? Greenway Biotech, Inc.: https://www.greenwaybiotech.com/blogs/news/whats-the-function-of-phosphorus-p-in-plants.

Teves, C. (2018, February 10). DENR raises urgency for mine rehabilitation. Philippine News Agency: https://www.pna.gov.ph/articles/1024785.

The Mosaic Company. (2020). Soil pH. CropNutrition: https://www.cropnutrition.com/efu-soil-ph.

Tulod, A., Castillo, A., Carandang, W., & Pampolina, N. (2012). Growth performance and phytoremediation potential of Pongamia pinnata (L.) Pierre, Samanea saman (Jacq.) Merr. and Vitex parviflora Juss. in copper-contaminated soil amended with zeolite and VAM. Asia Life Sciences, 21(2), 499-522.

Zhao, H., Li, X., Zhang, Z., Zhao, Y., Yang, J., & Zhu, Y. (2017). Species diversity and drivers of arbuscular mycorrhizal fungal communities in a semi-arid mountain in China. PeerJ, 5(e4155). doi:10.7717/peerj.4155.

 

Share this article