Influence of рН values of a nutrient medium on growth and morphological properties of strains Cladobotryum sp


D.G. Medvediev, N.A. Bisko, N.G. Kadnikova

Isolates of the plant pathogenic fungus Cladobotryum sp. were isolated from white button mushroom carpophores affected by cobweb mould, which were collected in different commercial mushroom farms of Ukraine. Influence of nutrient medium pH values on accumulation of mycelium biomass of 11 strains of Cladobotryum sp. was determined. It was determined that the optimal pH value for the growth of all strains is 6.9. It was found that depending on the acidity of the nutrient medium, the majority of strains synthesize pigments that stain mycelium of the fungus. Using the methods of confocal microscopy, we have studied the colour and conducted spectral analysis of autofluorescence of mycelial hyphae of different strains of Cladobotryum sp.

Keywords: Cladobotryum sp.; pH medium; mycelium; biomass; autofluorescence; hypha

Alekseeva, K. L. (2013). Species of the genus Cladethrium causing diseases of cultivated edible mushrooms. Problems of mycology and phytopathology in the KSHI century. Materials of the international scientific conference, dedicated to the 150th anniversary of the Corresponding Member of the INSSR, Professor Artur Arturovich Yachevsky. National Academy of Mycology, BGS, Design Studio "Dozor". SPb. Copy-R Group, 91-93. (in Russian).
Bhatt, N., & Singh, R. P. (1992). Cobweb disease of Agariucus bisporus: incidence, losses and effective management. Indian Journal of Mycology and Plant Pathology, 22(2), 178-181.
Bilay, V. I. (1982). Methods of experimental mycology. Reference book of science. Dumka, Kiev (in Russian).
Back, C. G., Lee, C. Y., Seo, G. S., & Jung, H. Y. (2012). Characterization of species of Cladobotryum which cause cobweb disease in edible mushrooms grown in Korea. Mycobiology, 40(3), 189-194.
Carrasco, J., Navarro, M. J., & Alegría, F. J. G. (2017). Cobweb, a serious pathology in mushroom crops: A review. Spanish journal of agricultural Research, 15(2), 19.
Chakwiya, A., Van der Linde, E. J., & Korsten, L. (2015). In vitro sensitivity testing of Cladobotryum mycophilum to carbendazim and prochloraz manganese. South African Journal of Science, 111(11-12), 1-7.
Dhar, G. M., & Seth, P. K. (1992). Factors influencing cobweb disease of Agaricus bisporus caused by Cladobotryum dendroides. Indian Journal of Mycology and Plant Pathology, 22(2), 178-181.
Fletcher, J. T., & Gaze, R. H. (2007). Mushroom pest and disease control: a colour handbook. CRC Press.
Gams, W., & Hoozemans, A. C. M. (1970). Cladobotryum-konidienformen von Hypomyces-arten. Persoonia-Molecular Phylogeny and Evolution of Fungi, 6(1), 95-110.
Gaze, R. (1995). Dactylium or cobweb. Mushroom Journal, (546), 23-27.
Jandaik, S., Guleria, D. S., Parmar, Y. S. (2004). Influence of cultural parameters on the growth and sporulation of Cladobotryum dendroides: the pathogen of cobweb on mushrooms. Science and cultivation of edible and medicinal fungi XVI, Penn. State, 503-505.
Medviedev, D., Bisko, N. (2018). The Influence of Temperature on Growth and Vitality of Mycelia of Fungicolous Fungus Cladobotryum dendroides Strains. Lesia Ukrainka Eastern European National University Scientific Bulletin Series: Biological Sciences, 4(377), 44-47. (in Ukraine).
McKay, G. J., Egan, D., Morris, E., Scott, C. and Brown, A. E. (1999). Genetic and morphological characterization of Cladobotryum species causing cobweb disease of mushrooms. Applied and Environmental Microbiology, 65, 606-610.
Mutumeenakshi, S., Mills, P. R. (1995). Detection and differentiation of fungal pathogens of bisporus . Science and Cultivation of Edible Fungi, Elliott (ed.) Balkema, Rotterdam.
Naumov, N. A. (1937). Methods of mycological and phytopathological studies. Moscow-Le Selkhozgiz. (in Russian).
Põldmaa, K. (2011). Tropical species of Cladobotryum and Hypomyces producing red pigments. Studies in Mycology, 68, 1-34.
Potočnik, I., Rekanović, E., Milijašević, S., Todorović, B., & Stepanović, M. (2008). Morphological and pathogenic characteristics of the fungus Cladobotryum dendroides, the causal agent of cobweb disease of the cultivated mushroom Agaricus bisporus in Serbia. Pesticidi i fitomedicina, 23(3), 175-181.
Rudakov, O. L. (1981). Mycophilic fungi, their biology and practical significance. The Science. Moscow (in Russian).

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