Influence of crop species on quantity and physiological activity of rhizosphere microorganisms


I. M. Malynovska, V. M. Yula, N. M. Asanishvili, M. M. Ptashnik, A. H. Lyubchich

Goal. Study of the number and physiological activity of the main trophic and systematic groups of microorganisms. Results. We registered that the rhizosphere soil under soybean plants had the maximum number of microorganisms associated with the transshipment in the root secretions of legume amino acids and amines. When soybean is grown on an intensive agricultural background, denitrifiers' physiological and biochemical activity is maximal. Among the studied cultures, their abundance is also maximal. In the corn rhizosphere, a minimal number of denitrifiers with a low physiological and biochemical activity are 8.2 times less than the physiological-biochemical activity of the row-spacing microorganisms that indicates the synthesis of substances with inhibitory properties of this culture relative to the denitrification process's pathogens. In the soil with extensive farming, the Azotobacter number considerably exceeds the indices of intensive-farming agrozem: when growing corn - in 3.26 times, wheat – 4.28, flax – 2.08, soya – 3.29, row-spacing – 4.05 times (organic and mineral fertilizers have not been introduced since 1987). This indicates the impossibility of using this indicator, as a diagnostic indicator, to sufficient soil fertility level. The number of polysaccharides synthesizing microorganisms in most crops' rhizosphere is higher when grown on an intensive agricultural background, except for winter wheat, in the rhizosphere of which the higher abundance of polysaccharide synthesizing microorganisms is shown on an extensive agricultural background. Conclusions. We established that the number and biochemical activity of microorganisms in soil depend on the type of crop and varies when growing plants on agricultural backgrounds of different intensity. We registered that in the rhizosphere of leguminous plants, the mineralization of humus slows down compared to cereal crops. The intensity of consumption of organic matter and humus is higher when growing the studied crops on an extensive agricultural background, the activity of mineralization of nitrogen compounds, on the contrary, when growing on an intensive agricultural background. The soil of soybean rows characterizes the minimum phytotoxicity, and the maximum is the soil of row-spacing and rhizosphere of wheat. Thus, we confirmed long-standing observations concerning the greater phytotoxicity of the rhizosphere with cereals than legumes.

Keywords: microorganisms, ecotrophical groups, nitrogen mineralization, humus mineralization rate, soil phytotoxicity, maize, wheat, soybean, agricultural background.



Canarini, A., Kaiser, C., Merchant, A., Richter, A., Wanek, W. (2019). Root exudation of primary metabolites: mechanisms and their roles in plant responses to environmental stimuli. Frontiers in Plant Science, 10, 157.

Dudman, W.F. (1977). The role of surface polysaccharides in natural environments.Surface carbohydrates of the prokaryotic cell.New York: Acad.Press, 1977.  P. 357–414.

Ivanov, V.P. (1973). Plants exudates and their significance in the life of phytocenosis. Moscow: Nauka (in Russian).

Jacoby, R. P., Chen, L., Schwier, M., Koprivova, A., & Kopriva, S. (2020). Recent advances in the role of plant metabolites in shaping the root microbiome. F1000Research, 9, F1000 Faculty Rev-151.

Jacoby, R. P., Koprivova, A. et all. (2021). Pinpointing secondary metabolites that shape the composition and function of the plant microbiome. Journal of Experimental Botany, 72(1), 57-69.

Korenblum, E., Dong, Y., Szymanski, J., Panda, S., Jozwiak, A., Massalha, H., Aharoni, A. (2020). Rhizosphere microbiome mediates systemic root metabolite exudation by root-to-root signaling. Proceedings of the National Academy of Sciences of the United States of America, 117(7), 3874-3883.

Kravchenko, L.V. (2000). The role of root exudates in integrating microorganisms with plants. Abstract of Doctor Dissertation. St. Petersburg (in Russian).

Kuzmicheva, Yu.V., Shaposhnikov, A.I., Azarova, T.S., Petrova, S.N., Naumkina, T.S., Borisov, A.Yu., Tikhonovich, I.A. (2014). Composition of root exometabolites of the highly symbiotic Triumph pea cultivar and its parental forms. Plant Physiology, 61(1), 121-128. 8DOI: 10.7868/S0015330314010084

Malinovska, I.M. (2011). Microbiological processes in the plants' rhizosphere of different crops. "Agricultural Microbiology: Achievements and Prospects": Collection of scientific proceedings. Special issue. Chernihiv, 113-126 (in Russian).

Malinovska, I.M., Dombrovska, I.V. (2011). State of microbiocenosis of gray forest soil at different use. Bulletin of Kyiv National University. Series Biology, 57, 21-25 (in Russian).

Malynovska, I.M. (2007). Functional role exopolysaccharides of soil microorganisms and plants. Agroecology Journal, 4, 53-64 (in Russian).

Malynovska, I.M., Bohovin, A.V., Ptashnik, M.M. (2009). Formation of soil microbiocenosis at the different ways of plant communities reproduction. Agriculture, 81, 105-118 (in Russian).

Malynovska, I.M., Kaminskyi, V.F., Tkachenko,  ??.A. (2020). Influence of heavy metals pollution on the formation of microbial community in gray forest soil. Ukrainian Journal of Ecology, 10 (6), 97-100. doi: 10.15421/2020_264

Meretskaya, E.F. (1999). Effect of agricultural crops on the formation of soil microorganisms in fields crop rotation and its productivity. Povolzhskiy Ecologicheskiy Zhurnal, 6, 136-139 (in Russian).

Parinkina, O.M., Klyueva, N.V., Petrova, L.G. (1993). Biological activity and effective soil fertility. Soil Science. 9, 76-81 (in Russian).

Samtsevich, S.A. (1965). Active excretion of plant roots. Plant physiology. 12 (5), 837-846 (in Russian).

Shaposhnikov, A.I., Morgunov, A.I., Akin, B., Makarova, N.M., Belimov, A.A. & Tikhonovich, I.A. (2016). Comparative characteristics of root systems and root exudation in synthetic, primitive and modern wheat varieties. Agricultural Biology, 51 (1). DOI: 10.15389 / agrobiology.2016.1.68rus (in Russian).

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