Isolation and molecular identification of Lactobacillus plantarum from intestinal samples of silver carp

Abstract

N.M. Hassan

The intestines of most animals contain bacterial species called lactobacilli. Lactobacillus plantarum is a microorganism that settles in the intestinal environment and inhibits the activities of useless microorganisms and pathogens (Andanil et al., 2012). Lactic acid bacteria are the most important probiotic microorganisms including various bacteria such as lactobacilli and Bifidobacterium (Marco et al. 2006). Lactobacilli are gram-positive, non-motile, spore-less and catalase negative bacilli that convert different sugars into lactate and acetate (Guy et al., 2014). L. casei and L. plantarum also have good antagonistic impacts on Staphylococcus aureus and Escherichia coli (Salehi, 2013). Probiotics can stabilize microbial balance in the intestinal environment by improving their health and usefulness. In addition, probiotics play an important role in maintaining the consumers' health through the synthesis of some essential nutrients (Behnsen et al., 2009; Mohammadian et al., 2014). The bacterium was identified by a molecular technique using a primer designed from the 16S rRNA gene to perform PCR reaction for the detection of Lactobacillus genera (Nour, 1998). The intestines from 50 fish specimens were sampled, among which 28 positive samples of L. plantarum were identified and isolated after staining and testing by PCR (Lotfi et al. 2010).

Keywords: Lactobacillus plantarum; silver carp; probiotic

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