Karyotypes and genome size of Adonis amurensis and Adonis apennina (Ranunculaceae) from Asian Russia


E. Yu. Mitrenina, A. S. Erst, M. V. Skaptsov, T. V. Leonova, T. N. Veklich, O. A. Chernysheva, A. N. Luferov, W. Wang

The karyotypes of Adonis amurensis from Amur Oblast,' and Adonis apennina from Altai Republic, Khakassia Republic, and Irkutsk Oblast' have been investigated. The karyotype formula was obtained as 2n = 2x =16 = 8m + 8sm(4sat) for all specimens. We estimated karyotype asymmetry through the calculation of the Coefficient of Variation of Chromosome Length (CVCL), Coefficient of Variation of Centromeric Index (CVCI), and Mean Centromeric Asymmetry (MCA), and determination of Stebbins asymmetry index. The chromosome set of Adonis amurensis was found to be more symmetrical than the chromosome set of Adonis apennina. The average absolute nuclear DNA content (2?value) was originally determined for Adonis amurensis and Adonis apennina by flow cytometry and attained on average 20.38 pg and 17.29 pg, respectively.   

Keywords: Adonis L., chromosomes, 2Cvalue, karyotype, nuclear DNA content, Ranunculaceae

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