Morphological and biochemical parameters of blood and peroxidation state in water buffalo transition period


H. Hryshchuk, I. Chala, L. Yevtukh, O. Pinsky, A. Revunets, Yu. Kovalchuk, V. Karpiuk, P. Kovalov, L. Kovalova, V. Zakharin, Ya. Veremchuk, M. Pobirskyi

The paper presents the results of the study of morphological and biochemical parameters of blood and the state of peroxidation in female water buffaloes during the transition period in the conditions of Western Ukraine. 10 pregnant buffalo cows aged 2–2.5 years with the live weight 480–530 kg, kept in the subsidiary "Play", Klymets Village, Lviv region, have been selected for the research. The gestation period at the beginning of the study was 8–8.5 months and milk productivity at the beginning of lactation made up 12–18 L per day. The analysis of the morphological parameters of the cows' blood showed that all indicators were within the reference range. After calving, the number of erythrocytes and leukocytes decreased significantly, while the number of platelets increased. The changes in the leukogram indicate the development of an inflammatory post-calving process. Calving itself led to a rise in the intensity of catabolic processes in the bodies of the cows, but these changes were within physiological limits, which justifies a high adaptability of buffaloes. A significant increase in the activity of lactate dehydrogenase and creatine kinase alongside with a decreased activity of other indicator enzymes has been established. An essential area of the research conducted was the identification of the interrelation between metabolites. In the dry spell, the highest degree (r?0.9) of the correlation was found between glucose and bilirubin. Also, a relatively high negative correlation was registered between the concentration rates of total protein. A high level of positive correlation was observed between Potassium concentration and creatine kinase (CK) activity. After calving, the relationships between the metabolites changed significantly, thus the highest degree of negative correlation was found between Potassium and urea. A high degree of positive correlation was found between the concentration rates of cholesterol and urea. A study of the lipid peroxidation intensity in the buffaloes' blood during the transition period showed that the concentration of both metabolites under research increased in the postpartum period: lipid hydroperoxides content by 80%, whereas malonic dialdehyde increased by 2.2 times. Since there was a progressive accumulation of malonic dialdehyde in the blood, these changes may indicate a certain intensity of the enzymatic reserves of the antioxidant system in cow-buffaloes in the postpartum period.

Key words: water buffalo cows, morphological, biochemical parameters of blood, lipid peroxidation



AbdEllah, M.R., Hamed, M.I., & Derar, R.I. (2013). Serum biochemical and haematological reference values for midterm pregnant buffaloes. Journal of Applied Animal Research, 41(3), 309-317. doi: 10.1080/09712/19.2013.782870.

Abdulkareem, T.A. (2013). Some hematological and blood biochemical profile of Iraqi riverine buffaloes (Bubalusbubalis) during different gestation periods. Journal of Buffalo Science, 2(2), 78-84. doi: 10.6000/1927-520X.2013.02.02.4.

Agudelo-Gómez, D.A., Agudelo-Trujillo, J.H., & Cerón-Muñoz, M.F. (2016). Selection index for meat and milk traits of buffaloes in Colombia. Livestock Science, 191, 6-11. doi: 10.1016/j.livsci.2016.06.016.

Ashmawy, N.A. (2015). Blood metabolic profile and certain hormones concentrations in Egyptian buffalo during different physiological states. Journal of Animal and Veterinary Advances, 10, 271-280. doi: 10.3923/ajava.2015.271.280.

Bomko, V., Kropyvka, Yu., Bomko, L., Chernyuk, S., Kropyvka, S., & Gutyj, B. (2018). Effect of mixed ligand complexes of Zinc, Manganese, and Cobalt on the Manganese balance in high-yielding cows during first 100-days lactation. Ukrainian Journal of Ecology, 8(1), 420–425. doi: 10.15421/2018_230

Borshch, O.O., Gutyj, B.V., Sobolev, O.I., Borshch, O.V., Ruban, S.Yu., Bilkevich, V.V., Dutka, V.R., Chernenko, O.M., Zhelavskyi, M.M., Nahirniak, T. (2020). Adaptation strategy of different cow genotypes to the voluntary milking system. Ukrainian Journal of Ecology, 10(1), 145-150.  doi: 10.15421/2020_23

Borshch, O.O., Ruban, S.Yu., Gutyj, B.V., Borshch, O.V., Sobolev, O.I., Kosior, L.T., Fedorchenko, M.M., Kirii, A.A., Pivtorak, Y.I., Salamakha, I.Yu., Hordiichuk, N.M., Hordiichuk, L.M., Kamratska, O.I., & Denkovich, B.S. (2020). Comfort and cow behavior during periods of intense precipitation. Ukrainian Journal of Ecology, 10(6), 98-102. doi: 10.15421/2020_265

Colakoglu, H.E., Yazlik, M.O., Kaya, U., Colakoglu, E.C., Kurt, S., Oz, B., Bayramoglu, R., Vural, M.R., & Kuplulu, S. (2017). MDA and GSH-Px activity in transition dairy cows under seasonal variations and their relationship with reproductive performance. Journal of Veterinary Research, 61(4), 497-502. doi: 10.1515/jvetres-2017-0067.

Couch, C.E., Movius, M.A., Jolles, A.E., Gormon, M.E., Rigas, J.D., & Beechler, B.R. (2017). Serum biochemistry panels in African buffalo: Defining reference intervals and assessing variability across season, age and sex. PLoS One, 12(5), e0176830. doi: 10.1371/journal.pone0176830.

Delfino, N.C., de AragãoBulcão, L.F., Alba, H.D.R., de Silva Oliveira, M.X., de Queiros, F.P.S., de Carvalho, G.G.P., Rennó, F.P., & de FraitasJúnior J.E. (2018). Influence of body conditions score at calving on the metabolic status and production performance of Murrah buffaloes (Bubalusbubalis) during the transition period. Assian-Australasion Journal of Animal Science, 31(11), 1756-1765. doi: 10.5713/ajas.17.0223.

Fadlalla, I.M.T., Omer, S.A., & Atta, M. (2020). Determination of some serum macroelement minerals levels at different lactation stage of dairy cows and their correlations. Scientific African, 8, e00351. doi: 10.1016/j.sciaf.2020.e00351.

Fiore, E., Giambelluca, S., Morgante, M., Contiero, B., Mazzotta, E., Vecchio, D., Vazzana, I., Rossi, P., Arfuso, F., Piccione, G., & Gianesella, M. (2017). Changes in some blood parameters milk composition and yield of buffaloes (Bubalusbubalis). Anim. Sci J., 88(12), 2025-2032. doi: 10.1111/asj.12872.

Grymak, Y., Skoromna, O., Stadnytska, O., Sobolev, O., Gutyj, B., Shalovylo, S., Hachak, Y., Grabovska, O., Bushueva, I., Denys, G., Hudyma, V., Pakholkiv, N., Jarochovich, I., Nahirniak, T., Pavliv, O., Farionik, ?., Bratyuk, V. (2020). Influence of "Thireomagnile" and "Thyrioton" preparations on the antioxidant status of pregnant cows. Ukrainian Journal of Ecology, 10(1), 122-126.  doi: 10.15421/2020_19

Gutyj, B., Stybel, V., Darmohray, L., Lavryshyn, Y., Turko, I., Hachak, Y., Shcherbatyy, A., Bushueva, I., Parchenko, V., Kaplaushenko, A., & Krushelnytska, O. (2017). Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load. Ukrainian Journal of Ecology, 7(4), 589–596

Gutyj, B.V., Mursjka, S.D., Hufrij, D.F., Hariv, I. I., Levkivska, N.D., Nazaruk, N.V., Haydyuk, M.B., Priyma, O.B., Bilyk, O.Y., & Guta, Z.A. (2016). Influence of cadmium loading on the state of the antioxidant system in the organism of bulls. Visnyk of Dnipropetrovsk University, Biology, Ecology, 24(1), 96–102.

Helmy, M.S., Masoud, H.M.M., Darwish, D.A., Abdel-Monself, M.M., & Ibrahim, M.A. (2019). Isolation and properties of lactate dehydrogenase isoenzyme from buffalo liver: Application in AST and ALT assay diagnostic kits. Journal of Applied Pharmaceutical Science, 17(4), 054-060. doi: 10.7324/JAPS.2019.91007.

Jerome, A., Srivastava, S.K., &Sharma, R.K. (2016). Study on follicular characteristics hormonal and biochemical profile in norgestomet + PMSG trated acyclic buffaloes. Iranian J. of Veterinary Research, 17(4), 247-252. doi: 10.22099/IJVR.2016.3910.

Kulyaba, O., Stybel, V., Gutyj, B., Turko, I., Peleno, R., Turko, Ya., Golovach, P., Vishchur, V., Prijma, O., Mazur, I., Dutka, V., Todoriuk, V., Golub, O. Dmytriv, O., Oseredchuk, R. (2019). Effect of experimental fascioliasis on the protein synthesis function of cow liver. Ukrainian Journal of Ecology, 9(4), 612-615

Marini, M.G., Perrini, C., Esposti, P., Corradetti, B., Bizzaro, D., Riccaboni, P., Fantinato, E., Urbani, G., Gelati, G., Cremonsi, F., & Lange-Consiglio, A. (2016). Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro. ReprodBiolEndocrinol, 14(1), 58. doi: 10.1186/s12958-016-0195-4.

Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of "Butaselmevit", Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 22–27.

Marutsova, V.J., Marutsov, P.D., & Binev, R.G. (2019). Evaluation of some blood liver parameters in cows with subclinical and clinical ketosis. Bulgarian Journal of Veterinary Medicine, 22(3), 314-321.doi: 10.15547/bjvm.2074.

Megahed, G.A., Anwar, M.M., Wasfy, S.I., & Hammadeh, M.E. (2008). Influence of heat stress on the cortisol and oxidant-antioxidants balance during oestrous phase in buffalo-cows (Bubalusbubalis): thermos-protective role of antioxidant treatment. Reprod. Domest. Anim., 43(6), 672-677. doi: 10.1111/j.1439-0531.2007.00968.X.

Mills, S.E., Beitz, D.C., & Young, J.W. (1989). Evidence for impaired metabolism in liver during induced lactation ketosis of dairy cows. Journal of Dairy Science, 69(2), 362-370. doi: 10.3168/jds.s0022-0302(86)80414-3.

Morales, A., Grob, D., Wittwer, F., Müller, A., Balocchi, O., & Pulido, R. (2016). Evaluation of blood metabolites in dairy cows grazing under two pasture allowances and supplemented with corn silage under restricted grazing conditions. RevistaBrasileira de Zootecnia, 45(11), 686-692. doi: 10.1590/s1806-9290201600/100007.

Naveena, B.M., & Kiran, M. (2014). Buffalo meat quality, composition, and processing characteristics: Contributon to the global economy and nutritional security. Animal Frontiers, 4(4), 18-24. doi: 10.2527/af.2014-0029.

Omidi, A., Fathi, M.H., & Parker, M. (2017). Alterations of antioxidant status markers in dairy cows during lactation and in dry period. Journal of Dairy Research, 84(1), 49-53. doi: 10.1017/S0022029916000753.

Pathan, M.M., Kaur, M., Mohanty, A.K., Kapila, S., & Dang, A.K. (2015). Comparative evaluation of neutrophil competence and activity of cows and buffaloes around peripartum. Journal of Applied Animal Research, 43(1), 61-68. doi: 10.1080/09712119.2014.896260.

Periši?, P., Bogdanovi?, V., Meki?, C., Ru?i?-Musli?, D., Stanojevi?, D., Popovac, M., & Stepi?, S. (2015). The importance of buffalo in milk production and buffalo population in Serbia Biotechnology in Animal Husbandry, 31(2), 255-263. doi: 102298/BAH1502255P.

Pilarczyk, B., Jankowiak, D., Tomza-Marciniak, A., Pilarczyk, R., Sablik, P., Drosd, R., Tylkowska, A., &Skólmowska, M. (2012). Selenium concentration and glutathione peroxidase (GSH-Px) activity in serum of cows at different stages of lactation. Biol. Trace Elem. Res., 147(1-3), 91-96. doi: 10.1007/s12011-011-9271-y.

Roman, L., Sidashova, S., Popova, I., Stepanova, N., Chornyi, V., Sklyarov, P., Koreyba, L., & Gutyj, B. (2020). The impact of lateral localization of the procedure on the effectiveness of transplations of pre-implantation embryos in heifers-recipient. Ukrainian Journal of Ecology, 10(6), 121-126. doi: 10.15421/2020_270

Rullo, R., Di Luccia, A., Ciani, E., & Pieragostini, E. (2019). The haemoglobin subunits alpha and beta: Old and new genetic variants in the Italian Mediterranean buffalo. Czech Journal Animal Science, 64, 279-290. doi: 10.17221/14/2018-CJAS.

Salzano, A., Spugnuolo, M.S., Lombardi, P., Vecchio, D., Limone, A., Censi, S.B., Balestriery, A., Pelagalli, A., & Neglia, G. (2017). Influence of different space allowance on reproductive performances in buffalo. Animal Reprod, 14(2), 429-436. doi: 10.21451/1984-3143-AR799.

Sattler, T., & Fürll, M. (2004). Creatine Kinase and Aspartate Aminotransferase in Cows as Indicators for Endometritis. Journal of Veterinary Medicine Series A, 51(3), 132-137. doi: 10.1111/j.1439-0442.2004/00612.x.

Shinde, P.L., Dass, R.S., & Gard, A.R. (2009). Effect of vitamin E and selenium supplementation on haemotology, blood chemistry and thyroid hormones in male buffalo (Bubalusbubalis) calves. Journal of Animal and Feed Sciences, 18(2), 241-256. doi: 10.22358/jafs/66388/2009.

Slivinska, L.G., Shcherbatyy, A.R., Lukashchuk, B.O., & Gutyj, B.V. (2020). The state of antioxidant protection system in cows under the influence of heavy metals. Regulatory Mechanisms in Biosystems, 11(2), 237-242. doi: 10.15421/022035

Slivinska, L.G., Shcherbatyy, A.R., Lukashchuk, B.O., Zinko, H.O., Gutyj, B.V., Lychuk, M.G., Chernushkin, B.O., Leno, M.I., Prystupa, O.I., Leskiv, K.Y., Slepokura, O.I., Sobolev, O.I., Shkromada, O.I., Kysterna, O.S., & ?usiienko, O.V. (2019). Correction of indicators of erythrocytopoesis and microelement blood levels in cows under conditions of technogenic pollution. Ukrainian Journal of Ecology, 9(2), 127-135.

Sundrum, A. (2015). Metabolic disorders in the transition period indicate that the dairy cows' ability to adapt is overstressed. Animals (Basel), 5(4), 978-1020. doi: 10.3390/ani5040395.

Urh, C., Deniβen, J., Gerster, E., Kraus, N., Stamer, E., Heitkönig, B., Spiekers, H., &Sauerwein, H. (2019). Short communication: Pro- and antioxidative indicators in serum of dairy cows during late pregnancy and early lactation: Testing the effects of parity, different dietary energy levels, and farm. Journal Dairy Sci., 102, 6672-6678. doi: 10.3168/jds.2019-16248.

Wang, X., Roy-Chowdhury, J., & Roy Chowdhury, N. (2006). Bilirubin metabolism: Applied physiology. Current Paediatrics, 16(1), 70-74. doi: 10.1016/j.cupe.2005.10.002.

Share this article