Phospholipid composition of blood plasma and internal organs of rats with diclofenac-induced hepatitis

Abstract

V.А. Gryshchenko, S.V. Sуsolyatin, J.V. Gulevata

Non-steroidal anti-inflammatory drugs, in particular sodium diclofenac, are characterized by direct cytotoxic action on hepatocytes in long-term use and in high doses. Therefore, the preparations of this group are used in experimental medicine and veterinary medicine for simulation of toxic liver damage in laboratory animals in determining the therapeutic effectiveness of preparations of hepatoprotective profile. Numerous cellular functions depend on the physicochemical properties of their lipid bilayer, the basis of which are phospholipids (PL). In the work the features of changes in the PL-composition of blood plasma and internal organs (liver, kidneys, heart and lungs) in Wistar line rats for artificial reproduction of toxic hepatitis at oral administration in the body of sodium diclofenac in a dose of 12.5 mg/kg body weight, one оnce a day, for 14 days. Thus, in patients with rats, there is a decrease in blood plasma content of both total (ТPL) and individual PL, namely: phosphatidyl ethanolamine (PE) and sphingomyelin (SM). In addition, these animals reduce the content of TPL in most internal organs: in the liver - by 21%, in the heart - by 19% and in the lungs - by 28%. Against this background, the content of phosphatidylserine (PS) and phosphatidylinositol (PI) decreases in the liver, in the heart - PS, PI, phosphatidic acid (PA) and cardiolipin (CL), in lungs - phosphatidylcholine (PC), PE and SM, and in the kidneys - PE and SM (increased content of lysoforms), proving the destructive effect of the drug on the cell membrane. Consequently, for diclofenac-induced hepatitis in rats, the similarity of quantitative changes in both the TPL and individual representatives in blood plasma and internal organs is established, which is expressed in the formation of their deficiency level and is important for the elucidation of the molecular foundations of the development of the pathological process. In general, this indicates the expediency of finding biologically active substances that stimulate the adaptation processes in cells for the negative effects of xenobiotics and compensate for the imbalance of PL in the development of hepatopathology.

Keywords: phospholipids; blood plasma; liver; kidneys; heart; lungs; laboratory rat; experimental toxic hepatitis; sodium diclofenac

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