Phylogeny of the North Asian Cystopteridaceae (Polypodiopsida) based on trnG-R intergenic spacer

Abstract

I.I. Gureyeva, D.O. Ulko, R.S. Romanets, A.A. Kuznetsov

The molecular phylogenetic analysis of the Cystopteridaceae family based on sequencing of the plastid DNA intergenic spacer trnG-R is carried out. The dataset includes sequences mainly of North Asian samples of both widerspread species and species described from the former USSR and modern Russia. The use of trnG-R intergenic spacer showed more comprehensive results for Gymnocarpium, than for Cystopteris. Gymnocarpium phylogeny includes four well-supported clades: the dryopteris clade, the robertianum clade, the continentale clade, and the jessoense clade. Data from trnG-R support the divergence between species with glabrous fronds  (G. dryopteris) and species with glandular-pubescent fronds. Among species having glandular-pubescent fronds, the robertianum clade is a sister to the remaining ones. Gymnocarpium fedtschenkoanum is more related to G. robertianum, than other glandular species. Our data confirmed the recognition of Gymnocarpium continentale and G. jessoense as distinct species. The topology of G. tenuipes has remained uncertain. Among Cystopteris species three highly supported clades are indicated: the montana clade, the sudetica clade and the Cystopteris fragilis complex. C. montana and C. sudetica occupy the clearest position in the phylogenetic tree. The topology of C. montana as sister to the remainder of the genus, and then the placement of C. sudetica as sister to the C. fragilis complex does not allow C. montana to be included together with C. sudetica in the same supraspecific taxon but allows to accept the genus Rhizomatopteris as distinct monotypic genus containing a single species – Rh. montana. Relationships among the taxa of C. fragilis complex including the species described from North Asia remain uncertain.

Key words: Cystopteris; Gymnocarpium; Cystopteridaceae; Plastid DNA; trnG-R intergenic spacer; Phylogeny

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