Secretory activity of cattle mammary gland tissues during lactation and duration of the interlactation period


M.D. Kambur, A.Y. Lermontov

Changes in the secretory activity of mammary gland during the involution period are designed to create conditions for inexhaustible use of the mammary gland, ensure the normal course of structural and functional regression for the development for the next lactation. For animals in which the interlactation period lasted less than 45 days, the absorption of non-esterified fatty acids in the last week of the involution period was 2.8 times higher than in animals in which the interlactation period lasted at least 55 days, 1.48 times higher than the absorption of acetic acid, 1.24 times higher than β-oxybutyric acid, 1.28 times higher than glucose, 2.82 times the total amount of phospholipids and triacylglycerols. For acetic acid, β-oxybutyric acid and glucose, not only higher absorption rates were observed, but also a tendency to increase the absorption level in the last weeks of lactation in animals with an interlactation period of less than 45 days, indicating metabolic changes in mammary gland tissue during the involution period. The duration of the dry period affects the secretory activity of breast tissue during lactation and the quality of milk produced during this period, which is expressed by lower fat content in the product while reducing the duration of the dry period to less than 45 days and reducing milk fat by 10.42% during the next calving. At the same time, the body weight of newborn calves in animals, whose interlactation period was less than 45 days by 19.3%, was lower than the animals of the control group, i.e., those who were in the interlactation period for at least 55 days, which indicates the negative impact of reducing the duration of the dry period on the body of animals.

Keywords:  Absorption; Non-esterified fatty acids; Acetic acid; β-oxybutyric acid; Milk; Animals


Accorsi, P. A., Pacioni, B., Pezzi, C., Forni M., Flint, D. J., & Seren, E. (2002). Role of prolactin, growth hormone and insulin-like growth factor 1 in mammary gland involution in the dairy cow. Journal of Dairy Science, 85, 507–513.  

Amos, M. R., Healey, G. D., Goldstone, R. J., Mahan, S. M., Düvel, A., Schuberth, H.-J., Sandra, ??., Zieger, P., Dieuzy-Labaye, I., Smith, G. E., & Sheldon, I. M. (2014). Differential endometrial cell sensitivity to a cholesterol-dependent cytolysin links trueperella pyogenes to uterine disease in cattle1. Biology of Reproduction, 90(3), 1–13.  

Cant, J. P., Trout, D. R., Qiao, F., & Purdie, N. G. (2002). Milk synthetic response of the bovine mammary gland to an increase in the local concentration of arterial glucose. Journal of Dairy Science, 85(3), 494–503.  

Cant, J. P., Madsen, T. G., & Cieslar, S. R. (2016). Predicting extraction and uptake of arterial energy metabolites by the mammary glands of lactating cows when blood flow is perturbed. Journal of Dairy Science, 99(1), 718–732.  

Cheong, S. H., Filho, O. G. S., Absalón-Medina, V. A., Pelton, S. H., Butler, W. R., & Gilbert, R. O. (2016). Metabolic and endocrine differences between dairy cows that do or do not ovulate first postpartum dominant follicles1. Biology of Reproduction, 94(1), 1–11.

Chew, B. P., Maier, L. C., Hillers, J. K., & Hodgson, A. S. (1981). Relationship Between Calf Birth-Weight And Dams Subsequent 200-Day And 305-Day Yields Of Milk, Fat, And Total Solids In Holsteins. Journal of Dairy Science, 64(12), 2401–2408.

Górová, R., Pavlíková, E., Blaško, J., Me?uchová, B., Kubinec, R., Margetín, M., & Soják, L. (2011). Temporal variations in fatty acid composition of individual ewes during first colostrum day. Small Ruminant Research, 95(2–3), 104–112.  

Gra?ner, D., Gilligan, G., Garvey, N., Moreira, L., Harvey, P., Tierney, A., & Zobel, R. (2015). Correlation between the milk vein internal diameter surface and milk yield in Simmental cows. Turkish Journal of Veterinary and Animal Sciences, 39, 741–744.

Greco, L. F., Neto, J. T. N., Pedrico, A., Ferrazza, R. A., Lima, F. S., Bisinotto, R. S., Martinez, N., Garcia, M., Ribeiro, E. S., Gomes, G. C., Shin, J. H., Ballou, M. A., Thatcher, W. W., Staples, C. R., & Santos, J. E. P. (2015). Effects of altering the ratio of dietary n-6 to n-3 fatty acids on performance and inflammatory responses to a lipopolysaccharide challenge in lactating Holstein cows. Journal of Dairy Science, 98(1), 602–617.  

Hammon, H. M., Stürmer, G., Schneider, F., Tuchscherer, A., Blum, H., Engelhard, T., Genzel, A., Staufenbiel, R., & Kanitz, W. (2009). Performance and metabolic and endocrine changes with emphasis on glucose metabolism in high-yielding dairy cows with high and low fat content in liver after calving. Journal of Dairy Science, Volume 92, Issue 4, 1554–1566.  

Jeong, W., Bae, H., Lim, W., Bazer, F. W., & Song, G. (2017). Adiponectin: A prosurvival and proproliferation signal that increases bovine mammary epithelial cell numbers and protects them from endoplasmic reticulum stress responses. Journal of Animal Science, 95(12), 5278– 5289.  

Kambur, M. D., Zamasiy, A. A., & Fedoruk, R. S. (2009). Physiology of lactation and digestion. Kozatsky Val, Sumy (in Ukrainian).

Lester, D. (1964). Determination of Acetic Acid in Blood and Other Tissues by Vaccuum Distillation and Gas Liquid Chromatography. Analytical Chemistry, 36(9), 1810–1812.  

Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.

Machado, V. S., Oikonomou, G., Bicalho, M. L. S., Knauer, W. A., Gilbert, R., & Bicalho, R. C. (2012). Investigation of postpartum dairy cows’ uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene. Veterinary Microbiology, 159(3–4), 460–469.

  Marey, M. A., Yousef, M. S., Kowsar, R., Hambruch, N., Shimizu, T., Pfarrer, C., & Miyamoto, A. (2016). Local immune system in oviduct physiology and pathophysiology: attack or tolerance? Domestic Animal Endocrinology, 56, 204–211.  

Mayasari, N., Rijks, W., de Vries Reilingh, G., Remmelink, G. J., Ducro, B., Kemp, B., Parmentier, H. K., & Van Knegsel, A. T. M. (2016). The effects of dry period length and dietary energy source on natural antibody titers and mammary health in dairy cows. Preventive Veterinary Medicine,127, 1–9.  

Miksa, I. R., Buckley, C. L., & Poppenga, R. H. (2004). Detection of nonesterified (free) fatty acids in bovine serum: comparative evaluation of two methods. Journal of Veterinary Diagnostic Investigation, 16, 139–144.

Parchem, K., Sasson, S., Ferreri, C., & Bartoszek, A. (2019). Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis. Free Radical Research, 53(1), 1068–1100.

Ribeiro, E. S., Santos, J. E. P., & Thatcher, W. W. (2016). Role of lipids on elongation of the preimplantation conceptus in ruminants. Reproduction, 152(4), R115–R126.  

Santos, J. E. P., Wiltbank, M. C., Ribeiro, E. S., & Bisinotto, R. S. (2016). Aspects and mechanisms of low fertility in anovulatory dairy cows. Animal Reproduction, 13(3), 290–299.  

Seeth, Mt., Hoedemaker, M., & Krömker, V. (2015). Physiological processes in the mammary gland tissue of dairy cows during the dry period. Berliner und Munchener Tierarztliche Wochenschrift, 128(1–2), 76–83.

Seymoura, W. M., Campbella, D. R., & Johnsonb, Z. B. (2005). Relationships between rumen volatile fatty acid concentrations and milk production in dairy cows: a literature study. Animal Feed Science and Technology, 119(1–2), 155–169.

Sheldon, I. M., Cronin, J., Goetze, L., Donofrio, G., & Schuberth, H. J. (2009). Defining postpar-tum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biology of Reproduction, 81(6), 1025–1032.

Sheldon, I. M., Cronin, J. G., Healey, G. D., Gabler, C., Heuwieser, W., Streyl, D., Bromfield, J. J., Miyamoto, A., Fergani, C., & Dobson, H. (2014). Innate immunity and inflammation of the bo-vine female reproductive tract in health and disease. Reproduction, 148(3), 41–51.

Swali, A., & Wathes, D. C. (2006). Influence of the dam and sire on size at birth and subsequent growth, milk production and fertility in dairy heifers. Theriogenology, 66(5), 1173–1184.  

Van Hoeij, R. J., Dijkstra, J., Bruckmaier, R. M., Gross, J. J., Lam, T. J. G. M., Remmelink, G. J., Kemp, B., & van Knegsel, A. T. M. (2017). The effect of dry period length and postpartum level of concentrate on milk production, energy balance, and plasma metabolites of dairy cows across the dry period and in early lactation. Journal of Dairy Science, 100(7), 5863–5879.  

Wang, B., Zhao, F. Q., Zhang, B. X., & Liu, J. X., (2016). An insufficient glucose supply causes reduced lactose synthesis in lactating dairy cows fed rice straw instead of alfalfa hay, Journal of Animal Science, 94(11), 4771–4780.  

Williamson, D. H., Mellanby, J., & Krebs, H. A. (1962). Enzymic determination of d(−)-β-hydroxybutyric acid and acetoacetic acid in blood. Biochemical Journal, 82(1), 90–96.

Zhao, F. Q. (2014). Biology of glucose transport in the mammary gland. Journal of Mammary Gland Biology and Neoplasia, 19, 3–17.

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