Study of fluvalinate residues in honey and wax after treatment of bee colonies in varroatosis

Abstract

T.F. Domatskaya, A.N. Domatsky

In three experiments on 18 bee colonies of the apiaries of Tyumen Region, the fluvalinate level in honey and wax after treatment of bee colonies with the following veterinary preparations has been studied: Tanis, Acaricidal bath, Varroplast M. Bee colonies were treated with drugs according to the application instructions. 7 days after treatment, wax and honey samples were taken from each colony and studied for active substance using gas-liquid chromatography. 36 wax samples and 18 honey samples were studied in total.A study does not reveal any fluvalinate residues in samples of honey and wax after treatment of bee colonies with Tanis. In bee colonies treated with Acaricidal bath, the fluvalinate level in wax selected from the brood frames averaged 0.97 ± 0.12 mg/kg, from honey frames - 0.12 ± 0.01 mg/kg in honey - 0.0026 ± 0.0002 mg/kg. A significant number of fluvalinate was found in bee colonies treated with Varroplast M. The fluvalinate level in wax samples from brood frames was 1.4 ± 0.09 mg/kg. Studies of wax taken from the honey frames indicated that there were fluvalinate residues of average 0.28 ± 0.08 mg/kg, the amount of drug in honey was 0.005 ± 0.001 mg/kg. It was found that the greatest number of fluvalinate residues is present in wax samples obtained from the brood frames. In the experiment with Acaricidal bath, the active substance level in wax from the brood combs significantly exceeded similar indicators of wax taken from honey frames (td=7.08). In the experiment with Varroplast M, the number of fluvalinate in wax samples taken from the brood frames is significantly higher than the data on wax from honey frames (td=9.3). Analysis of the results of own studies and literature data shows that the honey products should be monitored for acaricides residues, used to treat bee colonies in varroatosis in the apiaries of the Russian Federation.

Keywords: Bee colonies; varroatosis; fluvalinate; residues; honey; wax

References:
Al-Waili, N., Salom, K., Al-Ghamdi, A., & Ansari Mohammad, J. (2012). Antibiotic, Pesticide, and Microbial Contaminants of Honey: Human Health Hazards. The Scientific World Journal, Article ID 930849: 1-9. Doi: org/10.1100/2012/930849
Bilgili, A., & Selçuko??lu, E.(2017) The determination of the residues of amitraz and fluvalinate in honey samples obtained from Çukurova Region. The Turkish Journal of Occupational/Environmental Medicine and Safety 2, 1(2), 34.
Bonzini, S., Tremolada, P., Bernardinelli, I., Colombo, M., & Vighi, M. (2011). Predicting pesticide fate in the hive (part 1): experimentally determined τ-fluvalinate residues in bees, honey and wax. Apidologie, 42(3), 378-390. DOI: 10.1051/apido:200504).
Bogdanov, S., Kilchenmann, V., & Imdorf, A. (1999). Acaricide residues in honey, beeswax and propolis. Swiss Bee Research Centre, Dairy Research Station, Liebefeld, Bern, Switzerland, 11.
Domatskaya, T. F., & Domatsky, A. N. (2017). The drug Tanis efficiency in varroatosis and its influence on physiological state of bee colonies. Bulletin of KrasSAU, 1: 40-44
Domatskaya, T. F., Domatsky, A. N., & Dolnikova, T. Y. (2017) Study of the efficiency of «Varroplast M» at varroatosis. Michurinsk Agronomy Bulletin, 2, 123-126.
Domatskaya, T. F., & Schneider, A. A. (2009). Acaricidal mat - an effective remedy for the treatment bee colonies at varroatosis. Siberian Bulletin of Agricultural Science, 6, 70-73.
Gregorc, A., Alburaki, M., Sampson, B., Knigh, P., & Adamczyk, J. (2018). Toxicity of Selected Acaricides to Honey Bees (Apis mellifera) and Varroa (Varroa destructor Anderson and Trueman) and their use in controlling Varroa within honey bee colonies. Insects Journal, 9(55), 2-16. Doi:10.3390/insects9020055.
Guidelines for the determination of fluvalinate in honey and wax by gas-liquid chromatography, approved by the Ministry of Health of the USSR on July 29, 1991, No. 6223-91(1995) Moscow, Center for Scient and Techn Inform, Propaganda and Advertising, Collection No. 23, 89-94.
Tamim, A., Abdullah, H., & Khawla, M. (2011). Study of Treatment Methods of honeybee Colonies (Apis mellifera L.) with Different Pesticides, and their Residues in Some of Bee Products ( Honey and Beeswax). Journal for Research and Scientific Studies-Biological Science Series, 33(3), 144-160.
Tau Fluvalinate. Revised summary report. Committee for Veterinary Medicinal Products at the European Agency for the Evaluation of Medicinal Products, EMEA/MRL/021-REV1/95.
Tsigouri, A., Menkissoglu–Spiroudi, U., Thrasyvoulou, A., & Diamantidis G. (2003). Fluvalinate residues in Greek honey and beewax. Journal APIACTA, 38, 50-53.
Wallner, K.(1999). Varroacides and their residues in bee products. Apidologie Journal, 30, 235-248. DOI: 10.1051/apido:19990212
Wilmart, O., Legrève, A., Scippo, M-L., Reybroeck, W., Urbain, B., Dirk, de Graaf., Steurbaut, W., Delahaut, Ph., Gustin, P., Nguyen, B. K., & Saegerman, C. l. (2016). Residues in beeswax: a health risk for the consumer of honey and beeswax. Journal of Agriculture and Food Chemistry, 2(11), 1-50.
 

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