Symbiotic potential of snap beans (Phaseolus vulgaris L.) depending on biological products in agrocoenosis of the Right-Bank Forest-steppe of Ukraine


S.A. Vdovenko, G.V. Pantsyreva, I.I. Palamarchuk, H.V. Lytvyniuk

The topicality of the research is determined to the search for new approaches to the development of technological techniques for growing beans, taking into account the soil-climatic conditions of the Right-Bank Forest-steppe of Ukraine. The research on the dynamics of the formation and the symbiotic apparatus functioning has been carried out; the influence of the nodule bacterium’s new strains on the activity of the symbiotic apparatus formation in the snap bean agrocenosis (Phaseolus vulgaris L.) has been investigated. It is proved that the use of biopreparations provides an increase in interphase periods. At the same time, the influence of the complex application of inoculation with bacterial preparations of Azotophyte-l, Biomag, Biocomplex-BTU-l on the basic symbiotic productivity indexes of snap bean crops, in particular the number and weight of active bulbils, the active symbiotic potential and yield of beans long pod of sorts of early ripeness group were studied. It was established that the use of the bacterial preparation of Biocomplex-BTU-l contributes to the increase of the number and the mass of bulb formations on the root system of the Zironka sort. The usage of a bacterial preparation Biocomplex-BTU-l contributes to receiving 18.4 t/ha or 9.4 t/ha of crop rise, and the correlation between yield and studied indexes were determined. The usage of Azotophyte-l and Biomag for pre-crop preparations of bean seeds is ineffective.

Keywords: Snap beans; agrocenosis; tuberous bacteria; symbiotic productivity; yield

Schwartz, A., Ortiz, I., Maymon, M., Herbold, C., Fujishige, N., Vijanderan, J., William, V., Kayoko, H., Andrew, D., Erin, R. S., DeMason, D. & Ann, M. H. (2013). Bacillus simplex-a little known PGPB with anti-fungal activity-alters pea legume root architecture and nodule morphology when coinoculated with Rhizobium leguminosarum bv. viciae. Agronomy, 3(4), 595-620.
Chen, C., Bauske, E. M., Musson, G., Rodriguez-Kabana, R., & Kloepper, J. W. (1995). Biological control of Fusarium wilt on cotton by use of endophytic bacteria. Biological control, 5(1), 83-91.
Hamaoui, B., Abbadi, J., Burdman, S., Rashid, A., Sarig, S., & Okon, Y. (2001). Effects of inoculation with Azospirillum brasilense on chickpeas (Cicer arietinum) and faba beans (Vicia faba) under different growth conditions. Agronomie, 21(6-7), 553-560.
Osoro, N. O., Kawaka, F., Naluyange, V., Ombori, O., Muoma, J. O., Amoding, A., & Maingi, J. M. (2014). Effects of water hyacinth (Eichhornia crassipes [mart.] solms) compost on growth and yield of common beans (Phaseolus vulgaris) in Lake Victoria Basin. Eur Int J Sc Tech, 3, 173-186.
Li, J. H., Wang, E. T., Chen, W. F., & Chen, W. X. (2008). Genetic diversity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China. Soil Biology and Biochemistry, 40(1), 238-246.
Figueiredo, M. D. V. B., Seldin, L., de Araujo, F. F., & Mariano, R. D. L. R. (2010). Plant growth promoting rhizobacteria: fundamentals and applications. In Plant growth and health promoting bacteria (pp. 21-43). Springer, Berlin, Heidelberg.
Figueiredo, M. V. B., Martinez, C. R., Burity, H. A., & Chanway, C. P. (2008). Plant growth-promoting rhizobacteria for improving nodulation and nitrogen fixation in the common bean (Phaseolus vulgaris L.). World Journal of Microbiology and Biotechnology, 24(7), 1187-1193.
Petrychenko, V. F., Tihonovich, I. A., & Kots, S. J. (2012). Agricultural microbiology and balanced development of agro-ecosystems. Journal of Agricultural Science, (8), 5-11.
Petrychenko, V. F., Tihonovich, I. A., & Kots, S. J. (2012). Agricultural microbiology and balanced development of agro-ecosystems. Journal of Agricultural Science, (8), 5-11.
Antipchuk, A. F. (1994). Ecological aspects of selection of rhizobia and increase of the effectiveness of symbiosis. Physiology and Biochemistry of Crop Plants, 26(4), 315-333.
Beneduzi, A., Ambrosini, A., & Passaglia, L. M. (2012). Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents. Genetics and molecular biology, 35(4), 1044-1051.
Volkogon, V. V., Nadkernych, O. V., Krutyl, D. V., Kovalevskaya, T. M., Bondarenko, G. L. (2001). Biopreparations on the basis of tuber bacteria for increasing the yield of legumes. Methodology of Experimental Case in Vegetable and Melons. Kh, Osnov, p: 369.
Budwina, O. Y. (1997). Highly competitive strains of nodule bacteria-the basis of the effectiveness of biopreparations. Microbial Journal 59(4), 123-131.
Derevyansky, V. P. (2012). Efficiency of limestone fertilizers, microbial preparations and macro- and microelements on plant resistance to diseases and soybean yield. Forages and Fodder Production. 72, 68-76.
DSTU 4794 (2007). Bean, Growing technology. General terms. K Gosstandart of Ukraine, p: 10.
Duparque, M. (1996). Main history steps of the pea improvement. Grain Legum, 12, 18.
Auxtero, E., Madeira, M., & Parker, D. (2012). Extractable Al and soil solution ionic concentrations in strongly leached soils from Northwest Iberia: effects of liming. ISRN Soil Science, 2012.
Kots, S. Y., Berehovenko, S. K., Kirichenko, E. V. (2007). Features of the interaction of plants and nitrogen fixing microorganisms. NAS of Ukraine, Institute of Plant Physiology and Genetics. K Science Opinion, p: 315.
Gamalero, E., & Glick, B. R. (2011). Mechanisms used by plant growth-promoting bacteria. In Bacteria in agrobiology: Plant nutrient management (pp. 17-46). Springer, Berlin, Heidelberg.
Gupta, A., Gopal, M., & Tilak, K. V. B. (2000). Mechanism of plant growth promotion by rhizobacteria.
Hosseinpur, A. R., & Zarenia, M. (2012). Evaluating chemical extractants to estimate available potassium for pinto beans (Phaseolus vulgaris) in some calcareous soils. Plant Soil and Environment, 58(1), 42-48.
Hung, P. Q., & Annapurna, K. (2004). Isolation and characterization of endophytic bacteria in soybean (Glycine sp.). Omonrice, 12, 92-101.
Jangu, O. P., & Sindhu, S. S. (2011). Differential response of inoculation with indole acetic acid producing Pseudomonas sp. in green gram (Vigna radiata L.) and black gram (Vigna mungo L.). Microbiology Journal, 1(5), 159-173.
Kalra, Y. P. (1995). Determination of pH of soils by different methods: collaborative study. Journal of AOAC International, 78(2), 310-324.
Kloepper, J. W., Schroth, M. N., & Miller, T. D. (1980). Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology, 70(11), 1078-1082.
Margeson, J. H., Suggs, J. C., & Midgett, M. R. (1980). Reduction of nitrate to nitrite with cadmium. Analytical Chemistry, 52(12), 1955-1957.
Martyniuk, S., & Oroń, J. (2008). Populations of rhizobia in some Polish soils not planted with legumes. Ekologija, 54(3).
Volkogon, V. V., Zaryshniak, A. S., Grinyk, I. V. (2011). Methodology and practice of the use of microbial drugs in crop cultivation technologies. Kyiv: Agrarian Science. p: 153.
Mohamed, Z. K., El-Sayed, S. A., Radwan, T. E. E., & Abd, E. W. (2009). Potency evaluation of Serratia marcescens and Pseudomonas fluorescens as biocontrol agents for root-knot nematodes in Egypt. Journal of Applied Sciences Research, (January), 93-102.
Parmar, N., & Dadarwal, K. R. (1999). Stimulation of nitrogen fixation and induction of flavonoid‐like compounds by rhizobacteria. Journal of Applied Microbiology, 86(1), 36-44.
Patika, V. P. (1999). Microorganisms and alternative agriculture. Kyiv: Harvest, 176.
Rajendran, G., Patel, M. H., & Joshi, S. J. (2012). Isolation and characterization of nodule-associated Exiguobacterium sp. from the root nodules of fenugreek (Trigonella foenum-graecum) and their possible role in plant growth promotion. International journal of microbiology, 2012.
Scheer, H. (2004). Chlorophylls and carotenoids. Encyclopedia of Biological Chemistry, 430-437.
Tahmasebpour, B. (2013). The effects of helping bacteria (Pseudomonas spp.) in nitrogen green beans fixation and nodulation with Rhizobium leguminosarumby phaseoli. International Journal of Science Inventions Today, 2(2), 556-566.
Watson, M., & Mullen, R. (2007). Understanding soil tests for plant-available phosphorus. Unpublished.


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