The influence of biological products on the growth and development of sunflower plants (Helianthus annuus L.) in the northern steppe of Ukraine


O. I. Tsyliuryk, V. I. Horshchar, O. O. Izhboldin, ?. V. Kotchenko, M. Y. Rumbakh, A. S. Hotvianska, Y. V. Ostapchuk, V. H. Chornobai

To neutralize negative factors (excessive technogenic load, deterioration of water, nutrient regime, and humus state of the soil) and improve the nutritional system of sunflower plants, it is necessary to use more widely mineral, organic, and micronutrient fertilizers, plant growth regulators. Thanks to the regulatory mechanisms of biological products, the development of the leaf surface are enhanced, the main functions are essential for the life of sunflower plants, and they are activated by: membrane processes, cell division, respiration and nutrition, the activity of enzyme systems, photosynthesis, a branched root system with enhanced absorbing capacity is created at the growth period. The main goal of this work was to study the influence of the growth of regulatory substances, different in the direction of action, on the morphogenesis, growth, and development, and productivity of sunflower plants of different ripeness groups in the conditions of the Northern Steppe of Ukraine. Moreover, the determination of the most rational stimulants of the growth of sunflower plants, which ensure the resistance of plants to diseases and adverse environmental factors, optimal growth and development of plants, and contribute to obtaining high and sustainable yields of oilseed crops. The laying and carrying out of field experiments was carried out following the generally accepted methodology of experimental work. The experimental part of the work was performed during 2018–2020 on the scientific and research field of the National Scientific and Research Center of Dnipro State Agrarian and Economic University in the stationary experiment of the Plant Science Department in five-crop rotation pure steam – winter wheat – corn – barley – sunflower to study the effectiveness of modern elements in technologies for growing cereals, legumes, and oilseeds. Scientific studies have established that the formation of the maximum leaf surface area of sunflower was observed using the growth stimulant Tseron (0.5 L/ha) to 70.9–78.1 thousand m2/ha, or 5.5–10.2% more for control. Here, sunflower plants formed the largest diameter of the basket – 23–26 cm (11.5–30.4% more than the control), and the maximum number of seeds in 829–951 pcs. The highest weight of 1000 seeds was characteristic of the medium-early hybrid Sumico HTS – 54.0–60.0 g, and the lowest for the medium-late Subaro HTS – 51–55 g, due to the biological characteristics of the hybrids. Plant growth stimulants on sunflower contributed to an increase in crop yields 1.01–1.7 times. The most significant increase in grain for all hybrids was provided by the agent Tseron (0.5 L/ha) – 0.16–0.75 t/ha, or 8.2–43.3%. The application of restricted drugs Tseron (0.5 L/ha) and Architect (0.5 L/ha) contributed to the growth of oil content by 3–8 and 4–6 percentage points.

Keywords: sunflower; hybrids; biological products; leaf surface area; chlorophyll; yield capacity; seed quality



Abobaker, A. M., Bound, S. A., Swarts, N. D., & Barry, K. M. (2018). Effect of fertilizer type and mycorrhizal inoculation on growth and development of sunflower (Helianthus annuus L.). Rhizosphere, 6, 11–19.

Andrusevich, K. V., Nazarenko, M. M., Lykholat, T. Y., Grigoryuk, I. P. (2018). Effect of traditional agriculture technology on communities of soil invertebrates. Ukrainian Journal of Ecology, 8(1), 3340. doi: 10.15421/2017_184

Akuaku, J., Melnyk, A., Zherdetska S., Melnyk, T., Surgan, O., & Makarchuk, A. (2020). Yield and quality of confectionery sunflower seeds as affected by foliar fertilizers and plant growth regulators in the left-bank Forest-Steppe of Ukraine. Scientific Papers. Series A. Agronomy, 63(1), 155-165.

Bazaliy, V. V., Domaratsky, E. A., Dobrovolsky, A. V. (2016). Agrotechnical method of prolongation of photosynthetic activity of sunflower plants. Bulletin of Agrarian Science of the Black Sea Coast, 4(92), 77–84 (in Ukrainian).

Baylis, A., & Dickst, J. (1983). Investigations into the use of plant growth regulators in oilseed sunflower (Helianthus annuus L.) husbandry. The Journal of Agricultural Science, 100(3), 723–730. doi: 10.1017/S0021859600035516

Basile, B., Brown, N., Valdes, J. M., Cardarelli, M., Scognamiglio, P., Mataffo, A., Rouphael, Y., Bonini, P., & Colla, G. (2021). Plant-based biostimulant as sustainable alternative to synthetic growth regulators in two sweet cherry cultivars. Plants, 10, 619.

Caruso, G., De Pascale, S., Cozzolino, E., Giordano, M., El-Nakhel, C., Cuciniello, A., Cenvinzo, V., Colla, G., & Rouphael, Y. (2019). Protein hydrolysate or plant extract-based biostimulants enhanced yield and quality performances of greenhouse perennial wall rocket grown in different seasons. Plants, 8, 208.

Chena, L., Hub, W., Long, C., & Wang, D. (2021). Exogenous plant growth regulator alleviate the adverse effects of U and Cd stress in sunflower (Helianthus annuus L.) and improve the efficacy of U and Cd remediation. Chemosphere, 262, 127815 (in Ukrainian).

Dospekhov, B. A. (1985). Field experiment technique. Moscow, Agropromizdat (in Russian).

Domaratskiy, E. O., Bazaliy, V. V., Domaratskiy, O. O., Dobrovol'skiy, A. V., Kyrychenko, N. V., & Kozlova, O. P. (2018). Influence of mineral nutrition and combined growth regulating chemical on nutrient status of sunflower. Indian Journal of Ecology, 45(1), 126–129.

Domaratskyi, Y., Kaplina, A., Kozlova, O., Koval, N., & Dobrovolskyi, A. (2020). Economic justification for the use of biological fungicides and plant growth stimulants for growing sunflower. Independent journal of management & production (IJM&P). Special Edition (Baltic States), November. 11, 9. doi: 10.14807/ijmp.v11i9.1406.

Eremenko, O. A., Kalitka, V. V., Kalenska, S. M., & Malkina, V. M. (2018). Assessment of ecological plasticity and stability of sunflower hybrids (Helianthus annuus L.) in Ukrainian Steppe. Ukrainian Journal of Ecology, 8(1), 289–296 (in Ukrainian). doi: 10.15421/2018_216

Ernst, D., Kovár, M., & ?erný, I. (2016). Effect of two different plant growth regulators on production traits of sunflower. Journal of Central European Agriculture, 17(4), 9981012. doi: 10.5513/JCEA01/17.4.1804

Filippo, D., Herrera, D.A., Muñoz-Ochoa, M., Hernández–Herrera, R. M., & Hernández–Carmona, G. (2019). Biostimulant activity of individual and blended seaweed extracts on the germination and growth of the mung bean. Journal of Applied Phycology, 31, 2025–2037.

Islam, M. T., Ckurshumova, W., Fefer, M., Liu, J., Uddin, W., & Rosa, C. A. (2021). Plant based modified biostimulant (copper chlorophyllin), mediates defense response in Arabidopsis thaliana under salinity stress. Plants, 10, 625.

Kalenska, S. M., & Taran, V. H. (2018). Harvest index of corn hybrids, depending on plant density, fertilizing doses and weather conditions of growing. Plant Varieties Studying and Protection, 14(4), 415–421 (in Ukrainian).

Kalenska, S. M., & Stoliarchuk, T. A. (2018). Varietal features of oil linseed yield formation depending on sowing rate and inter-row spacing in the conditions of Right-bank Forest-Steppe zone of Ukraine. Plant Varieties Studying and Protection, 14(3), 302–310 (in Ukrainian).

Kalenska, S. M., Prysiazhniuk, O. I., Polovynchuk, O. Yu., & Novytska, N. V. (2018). Comparative characteristics of the growth and development of grain crops. Plant Varieties Studying and Protection, 14(4), 406–414 (in Ukrainian).

Kalenska, S. M., & Ryzhenko, A. S. (2020). Evaluation of weather conditions for growing sunflower (Helianthus annuus L.) in the northern part of the left-bank forest steppe of Ukraine. Plant Varieties Studying and Protection, 16(2), 162–172 (in Ukrainian).

Kocira, S., Hara, P., Szparaga, A., Czerwi?ska, E., Beloev, H., Findura, P., & Bajus, P. (2020). Evaluation of the effectiveness of the use of biopreparations as seed dressings. Agriculture, 10(4), 90. doi: 10.3390/agriculture10040090

Kozlova, L. M., Noskova, E. N., & Popov F. A. (2020). Assessment of the development of diseases of grain crops with resource-saving systems of soil cultivation and the use of biological products in adaptive landscape agriculture. Agricultural science of the Euro-North-East, 21(6), 721–732 (in Ukrainian). doi:

Kuryata, V. G., Poprotska, I. V., & Rogach, ?. ?. (2017). The impact of growth stimulators and retardants on the utilization of reserve lipids by sunflower seedlings. Regulatory Mechanisms in Biosystems, 8(3), 317–322.

Khromykh, N., Lykholat, Y., Shupranova, L., Kabar, A., Didur, O., Lykholat, T., & Kulbachko, Y. (2018). Interspecific differences of antioxidant ability of introduced Chaenomeles species with respect to adaptation to the steppe zone conditions. Biosystems Diversity, 26(2), 132–138. doi:

Kvak, V. M., Hanzhenko, O. M., Zykov, P. Y., & Khivrych, O. B. (2017). Method for determination of leaf area in miscanthus. Novìtnì agrotehnologìï [Advanced agritechnologies], 5 (in Ukrainian). doi: 10.21498/na.5.2017.122228

Lykholat, Y. V., Khromykh, N. O., Lykholat, T. Y., Didur, O. O., Lykholat, O. A., Legostaeva, T. V., Kabar, A. M., Sklyar, T. V., Savosko, V. M., Kovalenko, I. M., Davydov, V. R., Bielyk, Y. V., Volyanik, K. O., Onopa, A. V., Dudkina, K. A., & Grygoryuk, I. P. (2019a). Industrial characteristics and consumer properties of Chaenomeles Lindl. fruits. Ukrainian Journal of Ecology, 9(3), 132–137.

Lykholat, Y. V., Khromykh, N. O., Lykholat, T. Y., Bobrova, O. M., Didur, O. O., Lykholat, O. A., Sudak, V. M., Legostaeva, T. V., Gtytzaj, Z. V., Kabar, A. M., Savosko, V. M., Kovalenko, I. M., Davydov, V. R., Suvorova, K. M., Dudkina, K. A., Kolinko, O. M., & Grygoryuk, I. P. (2019b). Introduction success of less common species from the genus Berberis L. Ukrainian Journal of Ecology, 9(4), 634–640. doi: 10.15421/2019_801

Melnyk, A., Akuaku, J., Trotsenko, V., Melnyk, T., & Makarchuk, A. (2019). Productivity and quality of high-oleic sunflower seeds as influenced by foliar fertilizers and plant growth regulators in the left-bank forest-steppe of Ukraine. Agrolife scientific journal, 8(1), 167–174.

Olas, J. J., Fichtner, F., & Apelt, F. (2019). All roads lead to growth: imaging-based and biochemical methods to measure plant growth. Journal of Experimental Botany, 1(71), 11–21. doi: 10.1093/jxb/erz406

Pokhylenko, A., Lykholat, O. A., Didur, O. O., Kulbachko, Yu. L, & Lykholat, T. Y. (2019). Morphological variability of Rossiulus kessleri (Diplopoda, Julida) from different biotopes within the steppe zone of Ukraine. Ukrainian Journal of Ecology, 9(1), 176–182.

Polat, T., Özer, H., Öztürk, E., & Sefao?lu, F. (2017). Effects of mepiquat chloride applications on non-oilseed sunflower. Turkish Journal of Agriculture and Forestry, 41, 472–479. doi:10.3906/tar-1705-77

Ryzhenko, A. S., Kalenska, S. M., Prysiazhniuk, O. I., & Mokriienko, V. A. (2020). Yield plasticity of sunflower hybrids in the conditions of the left-bank forest-steppe of Ukraine. Plant Varieties Studying and Protection, 16(4), 402–406 (in Ukrainian).

Savosko, V., Lykholat, Yu., Domshyna, K., & Lykholat, T. (2018). Ekolohichna ta heolohichna zumovlenist poshyrennia derev i chaharnykiv na devastovanykh zemliakh Kryvorizhzhia [Ecological and geological determination of trees and shrubs' dispersal on the devastated lands at Kryvorizhya]. Journal of Geology, Geography and Geoecology, 27(1), 116–130. doi: 10.15421/111837

Sethy, H., Patra, S. K., & Mohanty, C. R. (2016). Effect of plant growth regulators on growth and flowering of ornamental sunflower. International Journal of Agricultural Science and Research, 6(3), 561568.

Steel, R. D., Torrie, J. H., & Dickey, D. (1997). Principle and procedure of statistics. A biometrical approach. 3rd. ed. New York, McGraw-Hill.

Spitzer, T., Bílovský J., & Kazda, J. (2018). Effect of using selected growth regulators to reduce sunflower stand height. Plant, Soil and Environment, 64, 324–329.

Szparaga, A., Kubon, M., Kocira, S., Czerwinska, E., Paw?owska, A., Hara, P., Kobus, Z., & Kwa´sniewski, D. (2019). Towards Sustainable AgricultureAgronomic and Economic Effects of Biostimulant Use in Common Bean Cultivation. Sustainability, 11, 4575.

Tsyliuryk, A. I., Shevchenko, S. M., Ostapchuk, Y. V., Shevchenko, A. M., & Derevenets-Shevchenko, E. A. (2018). Control of infestation and distribution of Broomrape in sunflower crops of Ukrainian Steppe. Ukrainian Journal of Ecology, 8(1), 487– 497 (in Ukrainian). doi: 10.15421/2017_240

Tkalich, Y., Tkalich, I., ?syliuryk, O., & Masliiov, S. (2019). Reserves for increasing the yield of sunflower seeds in the Ukrainian steppe. Agriculture & Forestry, 65(3), 105–114 (in Ukrainian). doi: 10.17707/AgricultForest.65.3.09

Yeremenko, O. A., Kalytka, V. V., & Kalenska, S. M. (2017). Influence of growth regulator on plant growth, development and yield formation of sunflower hybrids (F1) under the conditions of southern steppe zone of Ukraine. Plant Varieties Studying and Protection, 13(2), 141–149 (in Ukrainian).

Zhao, C., Liu, B., Piao, S., Wang, X., Lobell, D. B., Huang, Y., Huang, M., Yao, Y., Bassu, S., & Ciais, P. (2017). Temperature increase reduces global yields of major crops in four independent estimates. Proceedings of the National Academy of Sciences, USA, 114, 9326–9331.

Share this article