The inheritance of economically valuable features in the intraspecific hybridization of bean (?haseolus L)

Abstract

L.V. Golovan, I.V. Klymenko, S.V. Stankevych, Yu.V. Vasylieva, Yu.Yu. Chupryna, I.V. Zabrodina, L.V. Zhukova, V.V. Nazarenko, Yu.M. Belay

The detailed study of the variability range of the productivity components will allow conducting the effective selection of the perspective hybrid plants at earlier stages of their study and using them for the further breeding work, especially under the conditions of biosystems adaptation to the climate changes. It is known that the success of hybridization depends on the well-chosen parental forms. 8 samples of bean were involved into the intraspecific hybridization. The selection of the samples was performed on the basis of morphological, isoenzyme and RAPD analyses. According to the research methods the samples are genetically remote and differ morphologically. As the result of the conducted research and on the basis of the statistical analysis of the data concerning the inheritance of the productivity elements, the following combinations of the cross-breeding were identified: Dokuchaievska/Holberg, Dokuchaievska/UDO501722, Pervomaiska/UDO501722 and Pervomaiska/UDO501709 (most of the studied features in the hybrid populations are genetically determined and they have a positive heterosis effect). In most cases, we found a high degree of the transgressive forms, which were combined with their high frequency on the basis of “the number of beans per plant”, “the number of seeds from the plant” and “the mass of seeds from the plant”. The simultaneous combination of the high degree and frequency of the transgressions depends on the well-chosen parental components for the cross-breeding. It was established that the degree and frequency of the transgressive forms appearance were higher when the high level of the heterosis was observed. When conducting the heterosis selection (especially concerning the productivity), first of all, the attention should be paid to those combinations that exhibit a high heterosis effect by the number of beans per plant, the number of seeds from the plant and the mass of seeds from the plant. However, there are some cases when in the first generation of the hybrids there is a depression of the economically valuable signs, and in the subsequent generations, on the contrary, we observe an increase in the degree of these signs manifestation. It is precisely these cases we observed in the further study of the hybrid populations. The comparative estimation of the received data showed that between the various signs of the hybrids and parenalt forms, the most common is the correlative dependence of the low force. The analysis of the degree of the correlative relations between F1, F2 hybbrids and their parental forms of Ph. vulgaris species showed that both parental forms have strong, medium and weak correlative relations with the prevalence of the latter. In the first generation we have noted the dominance of weak correlative forces of relations; in addition there was a small number of strong relations between the investigated signs. In the next generation, the spectrum of the correlative relations level extends as a result of splitting, we observe three degrees of manifestation of the correlative relations tightness. In our case, the F2 hybrids are characterized by the presence of strong, medium and weak links with the prevalence of the latter.

Keywords: Heterosis; prevalence; gene; hybrid; cross-breeding; Phaseolus vulgaris L.

References:
Abramova, Z. V. (1992). Praktikum po genetike. Agropromizdat, Moskva (in Russian).
Basset, M. J. (2004) List of genes - Phaseolus vulgaris L. Ann Rep Bean Improv Coop, 47(1), 24.
Baudoin, J. P. (1988). L"amelioration de Phaseolus lunatus L. en zones tropicales. 1. Taxonomie, origine et evolution du genre Phaseolus et de l"espece etudiee. Bull Rech. agron. Gembloux, 23(3), 237-260.
Bezugla, O. M. (1999). Visota roztashuvannja bob??v na roslin?? kvasol?? – vazhliva selekc??jna oznaka. Selekc??ja ?? nas??nnictvo, 82, 74-78 (in Ukrainian).
Boudet, J. C. (1977). Origene et classification des expeces cultivees du genre Phaseolus. Bull Soc Roy Bot Beld, 110(1/2), 65-76. Available from: https://www.jstor.org/stable/20793672.
Chekalina, N. M. (1987). Metodicheskie ukazanija po izucheniju obrazcov mirovoj kollekcii fasoli. L. (in Russian).
Corrêa, A. M., Teodoro, P. E., Gonçalves, M. C., Santos, A., & Torres, F. E. (2016). Selection of common bean (Phaseolus vulgaris L.) genotypes using a genotype plus genotype x environment interaction biplot. Genet Mol Res, 15(3). doi: 10.4238/gmr.15038427.
Dospehov, B. A. (1985). Metodika polevogo opyta (s osnovami statisticheskoj obrabotki rezul'tatov issledovanij). Agropromizdat, Moskva (in Russian).
Elliot, L. H., Aaron, J. L., Jean-Luc, J., & Mark, E. (2009). Plant Breeding with Genomic Selection: Gain per Unit Time and Cost Sorrells. Crop breeding & Genetics, 50(5), 1681-1690. doi:10.2135/cropsci2009.11.0662.
Epimaki, M. K., Shree, P. S., & Paul, G. (1995). Genetic Control of the Domestication Syndrome in Common Bean. Crop Science, 36(4), 1037-1045.
Geovani, B. A., Ângela, de F. B. A., Magno, A. P. R., & Flávia, B. S. (2007). Phenotypic recurrent selection in the common bean (Phaseolus vulgaris L.) with carioca-type grains for resistance to the fungi Phaeoisariopsis griseola. Genetics and Molecular Biology, 30(3), 584-588 http://dx.doi.org/10.1590/S1415-47572007000400014.
Golovan', L. V., & Puz??k, V. K. (2011). Anal??z uspadkuvannja ta zcheplennja lokus??v, shho kodujut' morfolog??chn?? oznaki ta ??zofermenti u kvasol?? zvichajno?? (Phaseolus vulgaris L.). Selekc??ja ?? nas??nnictvo, 100, 225-237 (in Ukrainian).
Golovan', L. V., & Puz??k, V. K. (2011). Oc??nka m??nlivost?? zrazk??v kvasol?? zvichajno?? (Phaseolus vulgaris) za dopomogoju RAPD – marker??v. B??olog??ja roslin ta b??otehnolog??ja: mater??ali m??zhnar. konf. molodih uchenih, B??la Cerkva, 57 (in Ukrainian).
Golovan', L. V., & Puz??k, V. K. (2011). Vnutr??shn'o– ta m??zhvidovij pol??morf??zm ??zofermentnih sistem amerikans'ko?? grupi vid??v rodu Phaseolus L. Aktual'n?? problemi botan??ki ta ekolog????. Proceed. Int. Conf. Berezne (in Ukrainian).
Golovan', L. V., Puz??k, V. K., & Popov, V. M. (2011). M??nliv??st' fermentnih sistem u predstavnik??v rodu Phaseolus L. Genetichn?? resursi roslin (in Ukrainian).
Goncharenko, G. G., Silin, A. E., & Padutov, V. E. (1993). Issledovanie geneticheskoj struktury i urovnja differenciacii u Pinus sylvestris L. v central'nyh i kraevyh populjacijah Vostochnoj Evropy i Sibiri. Genetika, 29(12), 2019– 2037 (in Russian).
Gostimskij, S. A., Kokaeva, Z. G., & Bobrova, V. K. (1999). Ispol'zovanie molekuljarnih markerov dlja analiza genoma rastenij. Genetika, 35, 1538-1549 (in Russian).
Guy, F., & Hubert, B. (1988). Selection Methods in the Common Bean (Phaseolus vulgaris). Genetic Resources of Phaseolus Beans, pp: 503-542.
Guy, F., & Hubert, B. (1988). Genetic Resources of Phaseolus Beans: Their maintenance, domestication, evolution and utilization. Selection Methods in the Common Bean (Phaseolus vulgaris). doi 10.1007/978-94-009-2786-5_21.
Jai, H. C., & Elizabeth, S. (2012). Diallel analysis of the genetic variation in some quantitative traits in dry beans New Zealand. Journal of Agricultural Research, 28, 223-231. https://doi.org/10.1080/00288233.1973.10421139
Jones, N., Ougham, H., Thomas, H. (1997). Markers and mapping: we are all geneticists now. New Phytol, 137, 165-177.
Katalog mirovoj kollekcii VIR. Fasol'. Harakteristika obrazcov po aktivnosti ingibitorov tripsina, soderzhaniju belka v semenah i drugim hozjajstvenno cennym priznakam (2004). VIR, Sainct Petersburg, 745 (in Russian).
Kochieva, E. Z., Ryzhova, N. N., Hrapalova, I. A., & Puhal'skij, V. A. (2002). Ispol'zovanie metoda RAPD analiza v opredelenii geneticheskogo polimorfizma i filogeneticheskih svjazej u predstavitelej roda Lycopersicon (Tourn.) Mill. Genetika, 38(6), 874-880 (in Russian).
Konarev, V. G., Gavriljuk, I. P., & Gubareva, N. K. (2000). Identifikacija sortov i registracija genofonda kul'turnyh rastenij po belkam semjan. VIR, Sainct Petersburg (in Russian).
Konovalov, A. A., Moiseeva, E. A., & Goncharov, N. P. (2008). Analiz nasledovanija i sceplenija nekotoryh priznakov v hromosome 5R u rzhi Secale cereal L. Selekc??ja ?? nas??nnictvo, 96, 106-112.
Kozhuhova, N. Je., & Sivolap, Ju. M. (2006). Molekuljarnye markery v genetiko– selekcionnyh issledovanijah kukuruzy. Citologija i genetika, 40(5), 69.80.
Kwabena, D., Daniel, A., Hussein, M., Asrat, A., & Matthew, W. B. (2016). Evaluation of common bean (Phaseolus vulgaris L.) genotypes for drought stress adaptation in Ethiopia. The Crop Journal, 4(5), 367-376. https://doi.org/10.1016/j.cj.2016.06.007.
Malyshev, S. V., Vojlokov, A. V., & Korzun, V. N. (2005). Kartirovanie genoma rzhi (Secale cereale L.) s pomoshh'ju molekuljarnyh markerov. Vestnik Vogis, 9(4), 473-480 (in Russian).
Mitranov, L. (1997). Problemi,postizhenija i perspektivi na mezhduvidovata hibridizacija pri obiknovenija fasul Ph. vulgaris L. Selskostop. Nauka, 35(4/6), 11-12 (in Russian).
Molhova, E., & Coneva, S. (1986). Razrastvane na integumentalnija tapetum – bariera na nesovmestimostta pri hibridizacija na Phaseolus vulgaris L. i P. Lunatus. Genet. Selekcija, 19(4), 303-308 (in Russian).
Monik, E. L., João, B. d. S., Flávia, F. C., & Karla, R. C. (2011). Natural selection in common bean microsatellite alleles and identification of QTLs for grain yield. Electronic Journal of Biotechnology, 14(1). doi: 10.2225/vol14-issue1-fulltext-7.
Myounghai, K., & Paul, G. (2009). Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae). Theoretical and Applied Genetics, 118(5), 979-992. doi 10.1007/s00122-008-0955-4.
Nerinéia, D. R., Evandro, J., Sandra, M. M., Lindolfo, S., & Daniele, P. R. (2014). Selection of common bean lines with high grain yield and high grain calcium and iron concentrations. Ceres Rev. Ceres, 61(1), 77-83 http://dx.doi.org/10.1590/S0034-737X2014000100010
McClean, P. E., Burridge, J., Beebe, S., Rao, I. M., & Porch, T. G. (2011). Crop improvement in the era of climate change: an integrated, multi-disciplinary approach for common bean (Phaseolus vulgaris). Functional Plant Biology, 38(12), 927-933. http://dx.doi.org/10.1071/FP11102.
Shirokij un??f??kovanij klasif??kator Ukra??ni rodu Phaseolus L. (2004). Hark??v (in Ukrianian).
Singh, S. P., & Urrea, C. A. (1995). Inter-and intraracial hybridization and selection for seed yield in early generations of common bean, Phaseolus vulgaris L. Euphytica, 81(2), 131-137.
Stankevych, S.,V., Vasylieva, Yu. V., Golovan, L. V., Zabrodina, I. V., Lutytska, N. V., Nakonechna, Yu. O., Molchanova, O. A., Chupryna, Yu. Yu., & Zhukova, L. V. (2019). Chronicle of insect pests massive reproduction. Ukrainian Journal of Ecology, 9(1), 262-274.
Beebe, S. E., Rao, I. M., Cajiao, C., & Grajales, M. (2008). Selection for drought resistance in common bean also improves yield in phosphorus limited and favorable environments. Crop Science, 48(2), 582-592. doi:10.2135/cropsci2007.07.0404.
Rodrigues, T. B., & Santos, J. B. D. (2006). Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles. Genetics and Molecular Biology, 29(2), 345-352. http://dx.doi.org/10.1590/S1415-47572006000200024.
Geffroy, V., Sévignac, M., De Oliveira, J. C., Fouilloux, G., Skroch, P., Thoquet, P., Paul, G., Thierry, L., & Michel, D. (2000). Inheritance of Partial Resistance Against Colletotrichum lindemuthianum in Phaseolus vulgaris and Co-localization of Quantitative Trait Loci with Genes. Involved in Specific Resistance, 13(3), 287-296. https://doi.org/10.1094/MPMI.2000.13.3.287.
Volkodav, V. V. (2000). Metodika derzhavnogo sortoviprobuvannja s??l'skogospodars'kih kul'tur. Kyiv (in Ukrainian).
 

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