The Lipid Peroxidation Processes Intensity Of Xylotrophic Basidiomycetes In Submerged Culture

Abstract

A. V. Chaika, O. V. Fedotov

The aim of the study was to determine the lipid peroxidation (LP) intensity of xylotrophic basidiomycetes cultures under submerged fermentation on glucose-peptone medium. The materials were submerged mycelium and culture filtrate of 79 strains of 18 species xylotrophic basidiomycetes. Among the studied strains, 60 strains belong to the order Agaricales and 19 – to order Polyporales. Most of the strains (85%) were isolated from the fruiting bodies collected in different localities of Donetsk city and its region. The following methods were used. Oven-dry biomass was determined by gravimetric method and biomass increase and specific growth rate were calculated. The pH of the culture filtrate was determined by potentiometric method. The intensity of lipid peroxidation processes was estimated with the modified thiobarbituric acid test. The study concluded that among the studied strains high level of LP in the mycelium is typical for the F. velutipes cultures, particularly the F-vv, F-03 and F-1. The low intensity of LP in the mycelium was established for the F. fomentarius Ff-09, Ff-1201 strains and the L. edodes Le-2, Le-4, Le-6, Le-7 strains. The high content of LP products in the culture filtrate is typical in most Polyporales cultures, for example, in the strains of I. lacteus IL-1201, T. hirsuta Th-11, D. quercina Dq-08 and F. fomentarius T-10. Paucity of the LP products in the culture filtrate established for some strains of F. velutipes, for example, F-204, F-11, S. commune Sc-1102 strain and G. lucidum cultures. Direct dependence between the content of LP products in the mycelium and culture filtrate was not established. Calculated correlation coefficients for the investigated strains showed the dependence of the growth rates and the intensity of LP in the mycelium and culture filtrate. Selected strains with high growth rate and a significant LP level in the culture filtrate are promising in technologies of lignocellulosic wastes and xenobiotics biodegradation and environment bioremediation.

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