Treatment strategies for sheep with acute yellow athrophy of the liver caused by the fasciolosis


B.O. Chernushkin, V.V. Vlizlo, L.G. Slivinska, B.V. Gutyj, A.R. Shcherbatyy, I.A. Maksymovych, M.I. Leno, V.I. Rusyn, M.H. Lychuk, V.L. Fedorovych, B.O. Lukashchuk, H.O. Zinko, O.I. Prystupa

The article presents results of studies of acute yellow athrophy of the liver development in sheep with fasciolosis. The general condition of sick sheep was suppressed; they had polypnoe, tachycardia and hypotension of the rumen, the reticulum, and the omasum. Total protein content (p<0.001), due to albumins (p<0.001) and α-globulins (p<0.001) was decreased in the blood serum of sheep with fasciolosis, which indicates protein synthesis dysfunction in the liver. At the same time, iblood serum levels of γ-globulins (p<0.01), total bilirubin (p<0.001), urea (p<0.01) and creatinine content (p<0.001) were decreased. Increase of cytolytic (AST, ALT) and cholestatic (GGT, ALP) enzymes activity in the blood serum of sick sheep was also established. Histological examination of liver bioptates from live animals indicated fatty infiltration of the parenchyma and protein dystrophy. These changes in blood parameters and histological studies indicate disruption of basic functions and structure of the liver. After administration of anthehelmintic Rolenol to sheep with fasciolosis a decrease in albumins and α-globulins content was recorded. Accordingly, the content of γ-globulins in their blood increased. At the same time, the concentration of total and conjugated bilirubin increased, indicating diminished of hepatocyte function to conjugate and secrete pigment. Deworming led to an active increase of aminotransferases (AST, p<0.001, ALT; p<0.001) activity, as well as cholestatic (GGT and ALP) enzymes. Microscopic examination of sheep liver bioptates afterRollenol administration indicated an increase of structural changes in hepatocytes. Thus, in sheep with fasciolosis and after administration of anthelmintic Rolenol, basic functions and structure of the liver were disrupted, indicating the development of acute yellow athrophy of the liver. The treatment of sheep with acute yellow athrophy of the liver was conducted according to the following scheme: daily intravenous administration of a 10% glucose solution at a dose of 0.1 g/kg; subcutaneous administration of insulin at a dose of 0.25 U/kg of body weight; twice a day peroral administration of hepatoprotector Hepabene three capsules and brewer's yeast with selenium four pills per animal; intramuscular injection of Trivit at a dose of 1 ml once a week was administered. After six days of treatment, improvements of general condition and recovery of basic functions and structure of the liver were established. In the blood serum of sheep increased albumins content decreased concentrations of total and conjugated bilirubin, as well as enzyme activity – AST, ALT, GGT and ALP were detected. The content of urea and creatinine in the blood serum did not differ from clinically healthy sheep, which may indicate a normalization of liver and kidneys function. Histological examination of liver bioptates indicated the restoration of their structure. However, a six-day treatment of sheep with acute yellow athrophy of the liver did not lead to normalization of some parameters of functional status and liver structure. Therefore, a rehabilitation period of 20 days was appointed, during which conditions of keeping were improved, and dietary feed was added into ration. At the end of rehabilitation the general condition of sheep was satisfactory, basic functions of the liver (protein synthesis, pigment, carbohydrate) were stabilized, the activity of indicator enzymes – AST, ALT, GGT and ALP was normalized, indicating the restoration of the hepatobiliary system structure.

Key words: Sheep; Fasciolosis; Rolenol; Acute yellow athrophy of the liver; Treatment


Amer, S., ElKhatam, A., Zidan, S., Feng, Y., & Xiao, L. (2016). Identity of Fasciola spp. in sheep in Egypt. Parasites & Vectors, 9(1), 623. doi: 10.1186/s13071-016-1898-2.

Boyko, O. O., Zazharska, N. M., & Brygadyrenko, V. V. (2016). The influence of the extent of infestation by helminths upon changes in body weight of sheep in Ukraine. Visnyk of Dnipropetrovsk University. Biology, Ecology, 24(1), 3–7. doi: 10.15421/011601

Chernushkin, B. O., Maksymovych, I. A., & Vlizlo, V. V. (2010). Funktsionalnyi stan ta struktura pechinky ovets, khvorykh na toksychnu hepatodystrofiiu [Functional state and structure of sheep liver which are sick on toxical hepatodystrophy], Scientific mesenger of veterinary medicine. Bila Tserkva, 5(78), 196–199 (in Ukrainian).

Dakhno, I. S. (2001). Epizootolohiia, patohenez, etiotropna ta imunokorehuiucha terapiia pry fastsiolozi i dykrotseliozi zhuinykh tvaryn: avtoref. dys. na zdobuttia nauk. stupenia d–ra vet. nauk: spets. 03.00.18. Kharkiv (in Ukrainian).

Dovhii, Yu. Yu. (2005). Fastsioloz velykoi rohatoi khudoby v umovakh tryvaloho ionizuiuchoho vyprominiuvannia (epizootolohiia, patohenez ta likuvannia): avtoref. dys. na zdobuttia nauk. stupenia d–ra vet. nauk: spets. 16.00.11. K. (in Ukrainian).

Gutyi, B., Ostapiuk, A., Kachmar, N., Stadnytska O., Sobolev O., Binkevych V., Petryshak R., Petryshak O., Kulyaba, O., Naumyuk, A., Nedashkivsky, V., Nedashkivska, N., Magrelo, N., Golodyuk, I., Nazaruk, N., & Binkevych, O. (2019). The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver. Ukrainian Journal of Ecology, 9(3), 222–226.

Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., Magrelo, N., Zmiya, M., & Katsaraba, O. (2017). The impact of endogenous intoxication on biochemical indicators of blood of pregnant cows. Regulatory Mechanisms in Biosystems, 8(3), 438–443. doi: 10.15421/021768.

Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug ?mprolinsyl. Regulatory Mechanisms in Biosystems. 8(1), 41–45. doi: 10.15421/021708

Gutyj, B., Leskiv, K., Shcherbatyy, A., Pritsak, V., Fedorovych, V., Fedorovych, O., Rusyn, V., & Kolomiiets, I. (2017). The influence of Metisevit on biochemical and morphological indicators of blood of piglets under nitrate loading. Regulatory Mechanisms in Biosystems, 8(3), 427–432. doi: 10.15421/021766

Gutyj, B., Nazaruk, N., Levkivska, A., Shcherbatyj, A., Sobolev, A., Vavrysevych, J., Hachak, Y., Bilyk, O., Vishchur, V., Guta, Z. (2017). The influence of nitrate and cadmium load on protein and nitric metabolism in young cattle. Ukrainian Journal of Ecology, 7(2), 9–13. doi: 10.15421/2017_14

Gutyj, B., Paska, M., Levkivska, N., Pelenyo, R., Nazaruk, N., & Guta, Z. (2016). Study of acute and chronic toxicity of ‘injectable mevesel’ investigational drug. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 174–180. doi: 10.15421/201649.

Ivankiv, M., Kachmar, N., Mazurak, O., & Martyshuk, T. (2019). Hepatic protein synthesis and morphological parameters in blood of rats under oxidative stress and action of feed additive “Butaselmevit-plus”. Ukrainian Journal of Ecology, 9(4), 628-633.

Khariv, M. I., & Gutyj, B. V. (2016). Vplyv liposomal'nogo preparatu Butaintervit na protei'nsyntezuval'nu funkciju pechinky shhuriv za otrujennja tetrahlormetanom. Visnyk Dnipropetrovs'kogo universytetu. Biologija, medycyna, 7(2), 123–126 doi:10.15421/021622 (in Ukrainian).

Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematolohichni pokaznyky orhanizmu shchuriv za umov oksydatsiinoho stresu ta za dii liposomalnoho preparatu [Hematological indices of rat organisms under conditions of oxidative stress and liposomal preparation action]. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (1), 276–289. doi: 10.15421/201615 (in Ukrainian).

Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541. doi: 10.15421/2017_157

Knubben-Schweizer, G., & Torgerson, P. R. (2015). Bovine fasciolosis: control strategies based on the location of Galba truncatula habitats on farms. Vet Parasitol, 208(1–2), 77–83. doi: 10.1016/j.vetpar.2014.12.019.

Kulyaba, O., Stybel, V., Gutyj, B., Turko, I., Peleno, R., Turko, Ya., Golovach, P., Vishchur, V., Prijma, O., Mazur, I., Dutka, V., Todoriuk, V., Golub, O. Dmytriv, O., & Oseredchuk, R. (2019). Effect of experimental fascioliasis on the protein synthesis function of cow liver. Ukrainian Journal of Ecology, 9(4), 612–615.

Levchenko, V. I., Kondrakhin, I. P., & Vlizlo, V. V. (2012). Vnutrishni khvoroby tvaryn. Bila Tserkva, 1, 528. (in Ukrainian).

Levchenko, V. I., Vlizlo, V. V., & Kondrakhin, I. P. (2017). Klinichna diahnostyka khvorob tvaryn: pidruch. dlia studentiv vyshchykh navchalnykh ahrarnykh zakladiv. Bila Tserkva (in Ukrainian).

Levchenko, V.I., Vlizlo, V.V. ta in. (2019). Veterynarna klinichna biokhimiia: pidruchnyk 2-he vyd., pererob. ta dop. Bila Tserkva (in Ukrainian).

Macs, L., Lauvers, H., Deckers, W., & Vanparijs, O. (1988). Flukicidal action of closantel against immature and mature Fasciola hepatica in experimentally infected rats and sheep. Res Vet Sci, 44(2), 229–232.

Mann, S., Leal Yepes, F. A., Wakshlag, J. J., Behling-Kelly, E., McArt, J. A. A. (2018). The effect of different treatments for early-lactation hyperketonemia on liver triglycerides, glycogen, and expression of key metabolic enzymes in dairy cattle, Journal of Dairy Science, 101(2), 1626–1637. doi: 10.3168/jds.2017-13360.

Martyshuk, T. V., Gutyj, B. V., & Vishchur, O. I. (2016). Riven produktiv perekysnoho okysnennia lipidiv u krovi shchuriv za umov oksydatsiinoho stresu ta za dii liposomalnoho preparatu «Butaselmevit» [Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of ‘‘Butaselmevit’’], Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (2), 22–27. doi: 10.15421/201631 (in Ukrainian).

Mas-Coma, S., Bargues, M. D., & Valero, M. A. (2005). Fascioliasis and other plant-borne trematode zoonoses. International Journal for Parasitology, 35, 1255–1278. doi: 10.1016/j.ijpara.2005.07.010.

Matanovi??, K., Severin, K., Martinkovi??, F., Šimpraga, M., Janicki, Z., & Bariši??, J. (2007). Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica. Parasitol Res, 101(6), 1657???1661. doi: 10.1007/s00436-007-0709-2.

Mitchell, G. (2002). Update on fasciolosis in cattle and sheep. In Practice, 24, 378–385. doi: 10.1136/inpract.24.7.378.

Mooney, L., Good, B., Hanrahan, J. P., Mulcahy, G., & de Waal, T. (2009). The comparative efficacy of four anthelmintics against a natural acquired Fasciola hepatica infection in hill sheep flock in the west of Ireland. Veterinary Parasitology, 164(2–4), 201–205. doi: 10.1016/j.vetpar.2009.05.017.

Moritz, A. et al. (2014). Klinische Labordiagnostik in der Tiermedizin. Stuttgart: Schattauer GmbH.

Munita, M. P., Rea, R., Martinez-Ibeas, A. M. et al. (2019). Liver fluke in Irish sheep: prevalence and associations with management practices and co-infection with rumen fluke. Parasites Vectors, 12, 525. doi: 10.1186/s13071-019-3779-y.

Sheliakyn, Y. D., & Cheskydova, L. V. (2016). Yzmenenye pokazatelei krovy korov pry eksperymentalnom lechenyy fastsyoleza. Vestnyk Voronezhskoho hosudarstvennoho ahrarnoho unyversyteta, 1(48) doi: 10.17238/issn2071-2243.2016.1.45 (in Russian).

Simonov, M. R., & Vlizlo, V. V. (2013). Content of free amino acids and some parameters of the functional state of the liver in blood plasma of healthy and ketotic dairy cows. Folia Veterinaria, 57(3–4), 166–171.

Simonov, M. R., & Vlizlo, V. V. (2016). The effect of “Remivital” on plasma amino acid composition in dairy cows with ketosis. Agricultural Science and Practice, 3(1), 73–79. doi: 10.15407/agrisp3.01.073.

Simonov, M., & Vlizlo, V. (2015). Some blood markers of the functional state of liver in dairy cows with clinical ketosis. Bulgarian Journal of Veterinary Medicine, 18(1), 74-82. doi: 10.15547/bjvm.814.

Sobolta, A. H. (2009). Fastsioloz velykoi rohatoi khudoby (tsytohenetychni doslidzhennia za vplyvu fastsiolotsydnykh preparativ): avtoref. dys. na zdobuttia nauk. stupenia kand. vet. nauk: spets.16.00.11. Parazytolohiia, helmintolohiia. Kyiv (in Ukrainian).

Swan, G. E. (1999). The pharmacology of halogenated salicylanilides and their anthelmintic use in animals. Journal of the South African Veterinary Association, 70(2), 61-70. doi: 10.4102/jsava.v70i2.756.

Vázquez, A. A., Lounnas, M., Sánchez, J., Alba, A., Milesi, A., & Hurtrez-Boussès, S. (2016). Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba. Journal of Helminthology, 90(6), 719-725. doi: 10.1017/S0022149X15001029.

Vlizlo, V. V. (1997). Efektyvnist likuvannia koriv, khvorykh na zhyrovu dystrofiiu pechinky. Visnyk Bilotserkiv. derzh. ahrar. un-tu, 3(1), 37–41 (in Ukrainian).

Vlizlo, V. V. (1998). Zhyrovyi hepatoz u vysokoproduktyvnykh koriv: avtoref. dys. ... d-ra vet. nauk: 16.00.01; Kyiv. natsion. ahrar. un-t. K. (in Ukrainian).

Vlizlo, V. V., Chernushkin, B. O., Maksymovych, I. A., & Sobolta, A. H. (2012). Funktsionalnyi stan pechinky u ovets, khvorykh na fastsioloz, ta pislia zastosuvannia rolenolu. Nauk. visnyk vet. medytsyny: Zb. nauk. prats. Bila Tserkva, 10(99), 26–29 (in Ukrainian).

Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhen u biolohii tvarynnytstvi ta veterynarnii medytsyni: dovidnyk. Lviv: SPOLOM (in Ukrainian).

Vlizlo, V. V., Slivinska L. H., Maksymovych, I. A., Leno, M. I., & Halias, V. L. (2014). Laboratorna diahnostyka u veterynarnii medytsyni (dovidnyk). 2-he vydannia, pereroblene i dopovnene (in Ukrainian).

Vlizlo, V., & Lewtschenko, W. (1992). Beitrag zu den Leberkrankheiten der Mastbullen. Dtsch. Tierärztl. Wschr., 96, 254–257.

Share this article