Valorization of galacomannan of carob seeds (Ceratonia siliqua) by total heterogenous hydrolysis with protonated-kaolin and computer program simulation
Abstract
S. Bouanani* and A. Delimi
This study aimed to valorize galactomannan (GM) isolated from Carob seeds (Ceratonia silica). Galactose and mannose were produced by the hydrolysis of GM with various amount of kaolin catalysts (0.005 to 0.05g) treated by tompans solutions (pH 4, 5.5, 7, and 9) and under different formique acid/THF reaction mixture molarity (1M, 2M, 8M, 14M and 22M). The different hydrolysis conditions were critical to affect the hydrolysis efficiency of galactomannan when compared to conventional hydrolysis in 2M TFA. The amounts of total reducing sugars(TRS) released was was determined using Somogyi-Nelson method and simulated by computer program. Actived kaolin (pH=4) effectively depolymerized galactomann with a maximum content of 36.075μg/ml total reducing sugar. However, the reaction mixture molarity 1M was more effective in galacomannan hydrolysis (34.75μg/mlTRS). Based on the fitting of the set of experimental data, we noticed that the model reproduced the evolution of the simple sugars content generated during the hydrolysis in most studied parameters.