Vitamin C as ameliorating agent in phosphamidon treated chick embryos: A hematological study
Abstract
Pesticides used in agriculture and in public health to control insects, weeds and different vectors of diseases may also have adverse effects on the non-target living organisms. To delineate the toxicological profile on the broad spectrum organophosphate pesticide, Phosphamidon (Dimecron), the blood parameter has been analyzed in chick embryo in different period of epigenesis. The fertilized eggs were injected with 35 μg of dimecron in reference to control embryos which were injected with 0.9% normal saline solution. The ameliorating effect using vitamin C has also been considered in the present study. For hematological study, embryos were considered on 4th, 8th and 14th days of incubation to collect blood samples. Parameters like hemoglobin concentration (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), Mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), were analyzed. The hematological parameters showed reduction in number of erythrocytes, Hb level, PCV, MCH and MCHC. Increase in MCV suggests an intensified compensating activity of hematopoeitic system in response to the hemolytic action of the pesticide. The change in the blood picture due to the action of the pesticide and the protective role of ameliorative agent has been discussed.