Xeneostrogenes influence on cholinergic regulation in female rats of different age

Abstract

T.Yu. Lykholat, O.A. Lykholat, O.M. Marenkov, Yu.L. Kulbachko, I.M. Kovalenko, O.O. Didur

In view of the threat of adolescent children diet contamination, as a particularly sensitive population to chemicals with endocrine properties, an analysis of the occurrence of their health impairment risks in the future is necessary. The aim of the presented work was the study of the effect of exogenous estrogens on acetylcholinesterase activity in the organs of female rats of different ages. At the beginning of the experiment, the age of the experimental animals was 3 months-in the pubertant period and 6 months-mature ones. To modeling the effect of exogenous estrogen in vivo, rat’s food was treated with Synestrol in the calculation of 2 µg per kg for 45 days. In vitro, Sinestrol was added to the test samples at a concentration of 0.5 nmol/L, followed by incubation for 1 hour. At alimentary estrogen influence AChE study showed that in pubertal rats its activity is higher by 22% compared to the control indices in the brain. The enzyme activation was observed by 15% in the mature females.In the blood serum and liver tissue, the enzyme activity inhibition is determined. The study of the effect of synthetic estrogens on the activity of AchE in vitro did not reveal significant changes in the indices in all the studied tissues of animals of both experimental groups. Deterministic tension in the cholinergic mediator system of the brain against the background of AChE inhibition in the blood serum and liver tissue with predominance in the pubertal females in comparison with the affects in sexually mature rats indicates a lower efficiency of mediator transmission in the corresponding cholinergic neurons of the younger animals, which justifies the existence specific age-related physiological conditions determining high sensitivity to exogenous estrogen-like compounds.

Keywords: Xenoestrogens; acetylcholinesterase; in vivo; in vitro; brain health disorders

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