Comparative histochemical analysis of phosphatases activity of the skin glands in some artiodactyls


A.B. Kiladze, N.K. Dzhemukhadze

We determined the histochemical activity of phosphatases of sweat and sebaceous glands in the withers of three artiodactyls species — roe deer Capreolus capreolus  Linnaeus,  1758,  musk deer Moschus moschiferus Linnaeus,  1758,  and Saiga tatarica Linnaeus,  1766. Equal values of acid phosphatase activity  (60% level of relative activity),  alkaline phosphatase  (80% level of relative activity),  and adenosine triphosphatase  (100% level of relative activity) for both the sweat and sebaceous glands of roe deer founded. Only for alkaline phosphatase,  a 60% level of relative activity founded in the sweat glands of musk deer,  while the remaining parameters of phosphatase activity in both types of skin glands of musk deer showed zero values. Moderate levels of activity of acid phosphatase and alkaline phosphatase  (60% level of relative activity),  as well as an average level of adenosine triphosphatase activity  (80% level of relative activity) are typical for both the sweat and sebaceous glands of saiga. Synchronicity in the parameters of histochemical activity in both the sweat and sebaceous glands revealed,  which may be associated with physiological adaptation. Coordinate analysis based on digital analogues of the histoenzymatic activity of the skin glands made it possible to calculate the Euclidean distances between the studied species. In the sweat and sebaceous glands the following individual distances identified:   (1) distances between roe deer and musk deer are 5.92 and 7.07;  (2) distances between the roe deer and saiga are coincide and equal to 1.41 and 1.41;  (3) distances between musk deer and saiga are 5.00 and 5.83. Phenetic analysis,  which includes 18 parameters of histoenzymatic activity — three types of phosphatases in the sweat and sebaceous glands of three species of artiodactyls — proximity of the values ​​of roe deer and saiga at an obvious distance of the parameters of musk deer revealed. The Euclidean distances for the three species of artiodactyls were:   (1) distance between the roe deer and musk deer is 9.22;  (2) distance between roe deer and saiga is 2.00;  (3) distance between musk deer and saiga is 7.68.

Keywords: Histochemistry; Phosphatases; Analytical geometry; Cluster analysis; Artiodactyls; Ruminants; Pecora; Roe deer; Musk deer; Saiga


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