LIPID PEROXIDATION AND ENZYME ACTIVITY IN SOYBEAN UNDER COMPLEX ACTION OF FUNGICIDAL SUBSTANCES AND BRADYRHIZOBIUM JAPONICUM
The study of the peculiarities of biochemical processes that occur in the early stages of interaction of macro- and microsymbiotes is important to determine their participation in the formation of tolerance and realization of the symbiotic potential of established legume-rhizobial symbiosis under the influence of additional factors of various natures. The aim of the study was to investigate the effect of fungicidal substances in combination with nodule bacteria Bradyrhizobium japonicum on the accumulation of lipid peroxidation products, the activity of the key antioxidant enzyme ascorbate peroxidase and the effectiveness of nitrogenase enzyme complex in soybeans. Microbiological, physiological and biochemical research methods are used in the work. It was found that treatment of soybean seeds with fungicides in combination with inactive rhizobia induces a decrease in the content of lipid peroxidation products and an increase in ascorbate peroxidase activity in roots, which is accompanied by a slowing of nodulation processes in the early stages of ontogenesis. The use of fungicides together with active rhizobia, no significant changes in the accumulation of TBA-active products and increased activity of ascorbate peroxidase in soybean roots and nodules were recorded. This occurred along with the activation of the processes of nodulation and nitrogen fixation in soybean root nodules, formed by active rhizobia under the action of fungicides. It is concluded that the course of biochemical processes in the early stages of soybean-rhizobial symbiosis depends on the ability to realize the symbiotic properties of the strain Bradyrhizobium japonicum in combination with the active substances of fungicides.