Metabolic Profile Of Cow Blood Under The Treatment Of Ovaries Hypofunction By Hormonal And Phyto-preparations
S. B. Kornyat, M. M. Sharan, O. B. Andrushko, I. M. Yaremchuk
For the correction of reproductive function of cows with ovarian hypofunction practices use a number of hormones. Recently, to stimulate reproductive function using herbal medicines that have gonadotropic effect or stimulate secretion of steroid hormones who try to use to increase fertility. Therefore, we carried out an attempt to develop a method of regulation of reproductive function of the ovaries of cows using combination therapies that can provide effective treatment by studying the biochemical parameters of animals. The cows were divided depending on the treatment to control and two experimental groups of 5 animals in each group. Groups were formed by the following treatment regimens indicated pathology. Cows control group treated by next scheme: day 1-intramuscular injection drug in vitro at a dose of 10 ml; day 2-PMSG intramuscular administration of the drug at a dose of 500 IU; day 3-intramuscular injection drug Surfahon at a dose of 50 mg. Cows from experimental group 1 was injected intramuscularly liposomal drug based on herbal (Rhodiola rosea, Salvia); Animals from second experimental group were injected intramuscularly liposomal drug based on phyto-substances (Rhodiola rosea, Salvia) with gonadotropin-releasing hormone (Surfahon). Analysis of biochemical parameters of blood serum of cows with ovarian hypofunction found low concentrations of estradiol-17-β and progesterone. Between the control and experimental groups concentration of progesterone and estradiol-17-β differ within 10%, which indicates the same level of disease in all animals selected. Level carotene, ascorbic acid and cholesterol in all groups was within the physiological norm and differed slightly. It was established that the treatment of cows with hypofunction ovaries in the experimental group 1 progesterone level 7 days after treatment was 11.5, and 2 - on 41,4% (p <0,01) higher than in the control group animals, indicating that the revitalization of the ovary as a result of treatment and efficacy of liposomal drug on the basis of phyto-substances with gonadotropin-releasing hormone. The level of cholesterol on 7th day after treatment cows with ovarian hypofunction in the first experimental group was higher by 25,5% (p <0,05) and slightly exceeded the physiological value of this indicator in cows. In the second experimental group, it also exceed the physiological norm and exceeded the level in blood of cows in the control group at 18,6% (p <0,05), indicating the possibility of using cholesterol for the synthesis of progesterone. All the cows in the period from 30 to 60 days after the treatment have sign of oestrus and were artificially inseminated.