Morphogen development of in vitro cultivated Shrub roses

Abstract

I. S. Kosenko, L. A. Koldar, I. L. Denysko, O. A. Balabak, M. V. Nebykov, A. F. Balabak, A. V. Balabak

The article presents the study's results of the morphogen development features of the Shrub roses explants during in vitro reproduction — five cultivars from the collection fund of the National Dendrological Park "Sofiyivka" of the National Academy of Sciences of Ukraine: 'Gärtnerfreude' (W. Kordes' Söhne, 1991), 'Lavender Dream' (G. P. Ilsink, 1984), 'Pomponella' (W. Kordes' Söhne, 2005), 'Red Cascade' (R. S. Moore, 1976), 'Sommerabend' (W. Kordes' Söhne, 1995). The listed cultivars belong to those Shrub roses that form a dense cover of shoots, leaves, and flowers and are applicable for decorating plots that are of little avail for growing other ornamental plants. Shrub roses of most varieties introduced into the National Dendrological Park "Sofiyivka" National Academy of Science of Ukraine have shown sufficient adaptive ability in the natural and climatic conditions of the introduction area. Studies of gas resistance have shown that roses of this class adapt well to the conditions of the high content of traffic fumes and dust while maintaining high decorative qualities, which gave reason to recommend them for wide use in landscaping settlements of the Right-Bank Forest-Steppe Zone of Ukraine. However, the experience of introducing Shrub roses into gardening has shown that to create landscape expositions, it is often necessary to have a large amount of planting material of a specific cultivar, and to solve the problem requires the use of new, promising propagation methods. One of them is the in vitro plant propagation method, developed based on biotechnology to preserve plants genetically identical to the original maternal form, which contributes to the production of morphologically aligned, genetically homogeneous planting material. Therefore, the study of the reproduction features of the garden class Shrub roses, cultivated in vitro, defined the dependence of the explants morphogen development on changes in the concentrations of cytokinins and auxins included in the nutrient media are relevant and have both scientific and practical interest. The authors revealed that the studied cultivars were successfully introduced into the culture in vitro, are capable of direct morphogenesis when using exogenous phytohormones 6-BAP, β-IBA, β-IAA, 1-NAA; however, they had unequal indicators in the morphogenetic reactions display the degree. It was found that the phytohormonal regulation of morphogen processes in explants was determined by the presence of specific ratios of both auxins and cytokinins in the nutrient medium. Using segments of shoots with axillary and apical buds, the authors obtained morphogen structures that were formed directly on explants by the direct morphogenesis type. Of the tested inducers of morphogenesis, the most effective for the Shrub roses was MS 6-BAP – 2.0 mg/l and β-IBA – 0.01 mg/l to the nutrient medium. Within 28–34 days after passage on nutrient media modified with the addition of phytohormones, active formation of morphogen structures was observed, which gave rise to shoots, the number of which averaged from 2.92 to 12.04 pcs. per explant. 'Red Cascade' and 'Sommerabend' cvs differed in the maximum indices of adventive shoot formation, which, in six passages, reached a multiplication rate of 12.2±0.33 and 7.24±0.35, respectively. ‘Gärtnerfreude’, ‘Lavender Dream’, ‘Pomponella’ cvs. under the influence of various concentrations of phytohormones had slightly lower indices of morphogen potential with the multiplication rate in the range of 3.66±0.15 to 6.33±0.11. Thus, the differentiation of meristemic tissues of the studied Shrub rose cultivars with the subsequent induction of morphogenesis depends on the concentration of both cytokinins and auxins in nutrient media and determines the successful reproduction of these plants in vitro. A short reproduction cycle allows for a short time to obtain the required amount of planting material while maintaining the necessary economically valuable traits.

Key words: micropropagation, explants, nutrient medium, phytohormones, morphogenesis, multiplication rate|
 

References

 

Beals, P. (1997). Classic Roses: An Illustrated Encyclopedia and Grower's Manual of Old Roses, Shrub Roses and Climbers. London: Harvill Press.

Bhojwani, S.S., & Dantu, P.K. (2013). Plant tissue culture: An introductory text. New Delhi: Springer.

Bumbeeva, L.I. (2010). Rozy [Roses]. Moscow: Kladez'-Buks (in Russian).

Butenko, R.G. (1999). Biologiia kletok vysshikh rastenii in vitro i biotekhnologiia na ikh osnove [Cell biology of higher plants in vitro and biotechnology based on them]. Moscow: FBK-PRESS (in Russian).

Cairns, T. (2003). Horticultural Classification Schemes. In.: Roberts, A.V., Debener, T, & Gudin, S. (Eds.) Encyclopedia of Rose Science (Vol. 1, pp. 117–124). Elsevier.

Denysko, I.L. (2016). Troiandy patio. Bioloho-ekolohichni osoblyvosti, introduktsiia, perspektyvy vykorystannia u Pravoberezhnomu Lisostepu Ukrainy [Patio roses. Biological and ecological characteristics, introduction, prospects to use in the Right-Banc Forest-Steppe Zone of Ukraine]. Kyiv: Palyvoda A.V. (in Ukrainian).

Dixon, R.A., & Gonzales, R.A. (Eds.). (1995). Plant Cell Culture: A Practical Approach (2nd ed.). Oxford University Press, U.S.A.

Fomenko, T.I., & Maljush, M.K. (2010). Morphogenesis peculiarity and plant regeneration in vitro culture of narrow-leafed lupin (Lupinus angustifolius L.). Physiology and biochemistry of cultivated plants, 42(4), 306–314 (in Russian).

Gamburg, K.Z., Leonova, L.A., & Rekoslavskaia, N.Iu. (1978). Metabolizm  auksinov v roste kul'tur rastitel'nykh kletok [Metabolism of auxins in the growth of plant cell cultures]. In Kul'tura kletok rastenii [Plant cell culture] (pp. 47–52). Kyiv: Naukova dumka (in Russian).

Hrechanyk, R.?., Guz, M.M., & Oleksiychenko, N.?. (2011). Mulberry white (Morus alba Linn.) peculiarities introduction in culture in vitro. Scientific Bulletin of UNFU, 21(17), 9–21 (in Ukrainian).

Jacobi, K. (2002). Roses (A. Shackleton, Transl.). Bicester: Aura Books. (Original work published 1992).

Kalinin, F.L., Sarnatskaia, V.V., & Polishchuk V.E. (1980). Metody kul'tury tkanei v fiziologii i biokhimii rastenii [Tissue culture methods in plant physiology and biochemistry]. Kyiv: Naukova dumka (in Russian).

Kataeva, R.V., & Butenko, R.N. (1983). Klonal'noe mikrorazmnozhenie rastenii [Clonal micropropagation of plants]. Moscow: Nauka (in Russian).

Kefeli, V.Iu. (1966). Novye dannye ob endogennoi reguliatsii rosta rastenii [New data on endogenous regulation of plant growth]. Agricultural Chemistry, 7, 127–139 (in Russian).

Koldar, L.A. (2012). The role of phytohormones in the determination of Cerasus serratula Lindl. explants cultivated in vitro. News Biosphere Reserve "Askania Nova", 14, 152–155 (in Ukrainian).

Koldar, L.A., Nebykov, M.V., & Andrienko, O.D. (2015). Organogenesis induction from explants Amelanchier ovalis Medik. in vitro. Native and alien plant sciences, 11, 100–105 (in Ukrainian).

Koldar, L.A., Nebykov, M.V., & Denysko, I.L. (2009). Experimental organogenesis of patio roses in the conditions of in vitro culture. Native and alien plant sciences, 5, 114–118 (in Ukrainian).

Konvalyuk, I.I., Kravets, N.B., Drobyk, N.M., Mel’nyk, V.M., & Kunakh, V.A. (2005). Direct organogenesis in vitro of Gentiana lutea L. Biotechnologia Acta, 3(5), 66–73 (in Ukrainian).

Koval'chuk, I.Iu., Mukhitdinova, Z.R., Turdiev, T.T., Uspanova, G.K., & Chukanova, N.I. (2008, August). Mikroklonal'noe razmnozhenie maliny, kak metod sokhraneniia bioraznoobraziia rastenii v Kazakhstane [Micropropagation of raspberries as a method of preserving plant biodiversity in Kazakhstan]. In Biotekhnologiia kak instrument sokhraneniia bioraznoobraziia rastitel'nogo mira [Biotechnology as an instrument for vegetable world biodiversity conservation]. 2nd All-Russian Scientific and Practical Conference (Russia), Volgograd (p. 67) (in Russian).

Kruglova, N.N., & Seldimirova, O.A. (2013). The pathways of morphogenesis in vitro of wheat androclynic callus cells. Plant physiology and genetics, 45(5), 382–389 (in Russian).

Kudoiarova, G.R., Teplova, I.R., Dokicheva, R.A., Iusmanov I.Iu., & Veselov S.Iu. (2000, October). Vliianie benzil–6–aminopurina na rost i soderzhanie auksinov v prorostkakh pshenitsy i kukuruzy [Effect of benzyl-6-aminopurine on the growth and content of auxins in wheat and maize seedlings]. In Immunoanaliz reguliatorov rosta v reshenii problem fiziologii rastenii, rastenievodstva i biotekhnologii [Immunoassay analysis of growth regulators in solving problems of plant physiology, crop production and biotechnology]. 3d Conference (Russia), Ufa (p. 224).

Kunakh, V.A. (2005). Biotechnology of medicinal plants. Genetic, physiological and biochemical basis. Kyiv: Logos (in Ukrainian).

Kushnir, H.P., & Sarnats'ka V.V. (2005). Mikroklonal'ne rozmnozhennia roslyn. Teoriia i praktyka [Microclonal plant propagation. Theory and practice]. Kyiv: Naukova dumka (in Ukrainian).

Lavrentyeva, A.M. (2004). Application of biotechnologycal methods in propagation of ornamental plants introduced into Ukraine. Visnyk of L'viv University. Biological Series, 36, 137–145 (in Ukrainian).

Matskevych, V.V., Rohovs'kyj, S.V., Vlasenko, M.Yu., & Cherniak, V.M. (2010). Osnovy biotekhnolohii roslyn: navchal'nyj posibnyk [Plant biotechnology fundamentals: A study guide]. Bila Tserkva: BNAU (in Ukrainian).

Mattock, J. (1996). Gardener's Guide to Growing Roses. London: Reader's Digest.

Mel'nychuk, M.D., Novak, T.V., & Kunakh V.A. (2003). Biotekhnolohiia Roslyn [Plant biotechnology]. Kyiv: Polihraf Konsaltynh (in Ukrainian).

Mitrofanova, Iu.V. (2011). Somaticheskii embriogenez i organogenez kak osnova biotekhnologii polucheniia i sokhraneniia mnogoletnikh sadovykh kul'tur [Somatic embryogenesis and organogenesis as the basis of biotechnology for obtaining and preserving perennial horticultural crops]. Kyiv: Ahrarna nauka (in Russian).

Molkanova, O.I., Churikova, O.A., Konovalova, L.N., & Okuneva, I.B. (2002). Clonal micropropagation of the introduced varieties of Syringa vulgaris L. Herald of Moscow University. Series 16. Biology, 4, 8–14 (in Russian).

Moroz, O.K., & Denysko, I.L. (2010, October). Ekolohichni aspekty vykorystannia gruntopokryvnykh troiand v urboseredovyschi [Ecological aspects of Groundcover roses use in urban environment]. In Promyslova botanika: stan ta perspektyvy rozvytku [Industrial botany: state and development prospects]. 6th International Scientific Conference (Ukraine), Donetsk (pp. 313–315). Donetsk Botanical Garden NAS of Ukraine (in Ukrainian).

Moroz, O.K., & Denysko, I.L. (2014, June). Vykorystannia gruntopokryvnykh troiand u ozelenenni avtomobil'nykh dorih [Arrangement of highways with groundcover roses]. In Aktual'ni problemy ozelenennia naselenykh mists': osvita, nauka, vyrobnytstvo, mystetstvo formuvannia landshaftu [Problems of landscaping of populated areas: education, science, production, art of landscape formation]. 2nd International Scientific and Practical Conference (Ukraine), Bila Tserkva (pp. 70–73) (in Ukrainian).

Moroz, O.K., Denysko, I.L., & Bank, V.S. (2012). Collection of Shrub roses in the National Dendrological Park "Sofiyivka" NAS of Ukraine. News Biosphere Reserve "Askania Nova", 14, 181–185 (in Ukrainian).

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15(13), 473–497.

Nebykov, M.V., Koldar, L.A., & Derev'ianko, N.V. (2015, September). Rozmnozhennia Prunus laurocerasus in vitro [Reproduction of Prunus laurocerasus in vitro]. In Introduktsiya roslyn, zberezhennya ta zbahachennya bioriznomanittya v botanichnykh sadakh ta dendroparkakh [Plant introduction, conservation and enrichment of biodiversity in botanical gardens and arboreta]. International Scientific Conference (Ukraine), Kyiv (pp. 177–178). Fitosotsiotsentr (in Ukrainian).

Nebykov, M.V., Koldar, L.A., & Moroz, O.K. (2007). Rosa chinensis Jacq. minima (Sims) in culture in vitro. The Scientific Issues of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: biology, 3(33), 51–55 (in Ukrainian).

Orazbaeva, G.K., Khasanov, V.T., Iskhakov, A.R., & Shvidchenko, V.K. (2012). Klonal'noe razmnozhenie rastenii chernoi smorodiny (Ribes nigrum L.) in vitro [Clonal reproduction of black currant plants (Ribes nigrum L.) in vitro]. Science Bulletin of S. Seifullin Kazakh Agrotechnical University, 1(72), 115­–124 (in Russian).

Pilunskaia, O.A., & Bugara, A.M. (2001). Micropropagation of essential oil rose in vitro. Bulletin of the State Nikitsky Botanical Gardens, 83, 84-86 (in Russian).

Podvigina, O.A., Znamenskaia, V.V., & Frolova, V.V. (2001). Induktsiia rizogeneza u sakharnoi svekly v kul'ture in vitro [Induction of rhizogenesis in sugar beet in culture in vitro]. In Reguliatory rosta i razvitiia rastenii v biotekhnologii [Plant growth and development regulators in biotechnology]. 6th International Scientific Conference (Russia), Moscow (p. 160). Moscow Timiryazev Agricultural Academy (in Russian).

Rebrov, V.G., & Gromova, O.A. (2008). Vitaminy, makro- i mikroelementy [Vitamins, macro- and microelements]. Moscow: GEOTAR-Media (in Russian).

Romadanova, N.V., Seraj, N.A., Nurmanov, ?.?., & Karasholakova, L.N. (2017). Introduction of wild Malus sieversii apple into in vitro culture. Research, results, 3(75), 103–110 (in Russian).

Rugini, E.A., & Pesce, P.G. (2006). Genetic improvement of olive. Pomologia Croatica, 12(1), 43–74.

Valikhanova, G.Zh. (1996). Biotekhnologiia rastenii [Plant biotechnology]. Almaty: Konzhyk (in Russian).

Vetchinkina, E.M., & Mamayeva, N.A. (2005). Some aspects of the use of embryoculture of the genus Iris L. Bulletin Taras Shevchenko National University of Kyiv. Series Introduction and Conservation of Plant Diversity, 9, 15–16 (in Russian).

Vlasenko, M.Yu., Matskevych, V.V., Dul'niev, P.H., & Kozak, A.L. (2009, June). Determinatsiia ontohenezu roslyn kartopli v umovakh in vitro syntetychnymy fitohormonamy klasu tsytokininiv [Ontogenesis determination of potato plants in vitro by synthetic phytohormones of cytokinin class]. In Innovatsijni ahrotekhnolohii v umovakh hlobal'noho poteplinnia [Innovative agricultural technologies in the context of global warming] International Scientific and Practical Conference (Ukraine), Melitopol, Kyrylivka, 1, 24–25. Tavria State Agrotechnological University (in Ukrainian).

Vysotskii, V.A. (1986). Klonal'noe mikrorazmnozhenie rastenii. Kul'tura kletok rastenii i biotekhnologiia [Clonal micropropagation of plants. Plant cell culture and biotechnology]. Moscow: Nauka (in Russian).

Zelenina, H.A. (2005). Effect of nutrient medium components on micropropagation efficiency of Arnica foliosa Nutt. in vitro. The Bulletin of Kharkiv National Agrarian University. Series biology, 2(7), 89–93 (in Ukrainian).

Zhuravlev, Iu.N., & Omel'ko, A.M. (2008). Morfogenez u rastenii in vitro [Plant morphogenesis in vitro]. Plant Physiology, 55(5), 643–664 (in Russian).

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