The Influence Of Carbon And Nitrogen Sources On Xenobiotics Biodegradation By Xylotrophic Basidiomycetes Cultures
Abstract
A. V. Chaika, O. V. Fedotov
The effect of different carbon and nitrogen nutrition sources on the efficiency of xenobiotic biodegradation was investigated by means of submerged xylotrophic basidiomycetes cultures. We cultivated the prospective strains of xylotrophic basidiomycetes like Flammulina velutipes F-1105, Pleurotus eryngii P-er, Trametes hirsuta Th-11, and Daedalea quercina Dq-08 on the lab shaker on modified glucose-peptone medium. Some 24 of the carbon and 15 of the nitrogen nutrition sources were investigated. Absolutely dry biomass of the mycelium was determined by weighting method. The pollutants biodegradation efficiency of submerged cultures was determined by a modified method on a model compound – Methyl Orange. The individual carbon- and nitrogen-containing components of the modified glucose-peptone medium, the cultivation on which provides induction of model compound Methyl Orange biodegradation efficiency of the strains was also determined. They mostly were the carbon sources like glucose, fructose, xylose, starch, glycerol, PEG -1500, and the nitrogen sources like urea and peptone. It is suggested that the biodegradation efficiency of induction could caused either by inductive action of the nutrient component on metabolism of culture or by the manifestation of an adaptive response to adverse conditions.
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